Experiment: growth in full media supplemented with 5 .M CuSO4 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS18690 and SGL_RS18695 are separated by 48 nucleotides SGL_RS18695 and SGL_RS04310 are separated by 125 nucleotides SGL_RS04310 and SGL_RS04315 are separated by 162 nucleotides SGL_RS04315 and SGL_RS04320 are separated by 22 nucleotides
SGL_RS18690: SGL_RS18690 - DUF4351 domain-containing protein, at 520,682 to 520,840
_RS18690
SGL_RS18695: SGL_RS18695 - transposase, at 520,889 to 521,357
_RS18695
SGL_RS04310: SGL_RS04310 - MlaD family protein, at 521,483 to 522,853
_RS04310
SGL_RS04315: SGL_RS04315 - ABC transporter ATP-binding protein, at 523,016 to 523,798
_RS04315
SGL_RS04320: SGL_RS04320 - hypothetical protein, at 523,821 to 524,093
_RS04320
Position (kb) 521
522
523 Strain fitness (log2 ratio) -1
0
1
2
3 at 520.725 kb on + strand, within SGL_RS18690 at 520.741 kb on - strand, within SGL_RS18690 at 520.851 kb on - strand at 520.987 kb on - strand, within SGL_RS18695 at 521.044 kb on + strand, within SGL_RS18695 at 521.058 kb on + strand, within SGL_RS18695 at 521.093 kb on + strand, within SGL_RS18695 at 521.160 kb on - strand, within SGL_RS18695 at 521.570 kb on + strand at 521.650 kb on + strand, within SGL_RS04310 at 521.665 kb on - strand, within SGL_RS04310 at 521.665 kb on - strand, within SGL_RS04310 at 521.718 kb on + strand, within SGL_RS04310 at 521.833 kb on + strand, within SGL_RS04310 at 521.841 kb on - strand, within SGL_RS04310 at 521.841 kb on - strand, within SGL_RS04310 at 521.854 kb on - strand, within SGL_RS04310 at 522.090 kb on - strand, within SGL_RS04310 at 522.093 kb on + strand, within SGL_RS04310 at 522.381 kb on + strand, within SGL_RS04310 at 522.669 kb on - strand, within SGL_RS04310 at 522.703 kb on + strand, within SGL_RS04310 at 522.710 kb on - strand, within SGL_RS04310 at 522.767 kb on + strand at 522.846 kb on - strand at 522.852 kb on - strand at 522.873 kb on - strand at 522.970 kb on - strand at 523.194 kb on - strand, within SGL_RS04315 at 523.267 kb on - strand, within SGL_RS04315 at 523.350 kb on + strand, within SGL_RS04315 at 523.397 kb on - strand, within SGL_RS04315 at 523.579 kb on + strand, within SGL_RS04315 at 523.615 kb on + strand, within SGL_RS04315 at 523.723 kb on + strand at 523.723 kb on + strand at 523.731 kb on - strand at 523.757 kb on + strand at 523.762 kb on + strand at 523.783 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media supplemented with 5 .M CuSO4 but diluted to prevent light attenuation remove 520,725 + SGL_RS18690 0.27 +0.6 520,741 - SGL_RS18690 0.37 +0.2 520,851 - +0.1 520,987 - SGL_RS18695 0.21 -0.5 521,044 + SGL_RS18695 0.33 -0.7 521,058 + SGL_RS18695 0.36 -0.2 521,093 + SGL_RS18695 0.43 +0.6 521,160 - SGL_RS18695 0.58 -0.0 521,570 + +2.4 521,650 + SGL_RS04310 0.12 +2.0 521,665 - SGL_RS04310 0.13 +2.5 521,665 - SGL_RS04310 0.13 +0.8 521,718 + SGL_RS04310 0.17 +2.2 521,833 + SGL_RS04310 0.26 +2.4 521,841 - SGL_RS04310 0.26 +2.4 521,841 - SGL_RS04310 0.26 +2.3 521,854 - SGL_RS04310 0.27 +3.5 522,090 - SGL_RS04310 0.44 +1.6 522,093 + SGL_RS04310 0.44 +2.2 522,381 + SGL_RS04310 0.65 +2.0 522,669 - SGL_RS04310 0.87 +2.4 522,703 + SGL_RS04310 0.89 +2.4 522,710 - SGL_RS04310 0.89 +2.6 522,767 + +2.5 522,846 - +2.5 522,852 - +2.5 522,873 - +2.4 522,970 - +1.4 523,194 - SGL_RS04315 0.23 +2.8 523,267 - SGL_RS04315 0.32 +1.8 523,350 + SGL_RS04315 0.43 +2.0 523,397 - SGL_RS04315 0.49 +2.4 523,579 + SGL_RS04315 0.72 +2.7 523,615 + SGL_RS04315 0.77 +1.8 523,723 + +1.5 523,723 + +2.5 523,731 - +2.5 523,757 + +2.1 523,762 + +2.0 523,783 - +1.8
Or see this region's nucleotide sequence