Experiment: growth in full media supplemented with 5 .M CuSO4 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS00545 and SGL_RS00550 are separated by 154 nucleotides SGL_RS00550 and SGL_RS00555 are separated by 3 nucleotides SGL_RS00555 and SGL_RS00560 are separated by 20 nucleotides SGL_RS00560 and SGL_RS00565 are separated by 147 nucleotides
SGL_RS00545: SGL_RS00545 - alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB, at 106,537 to 106,950
_RS00545
SGL_RS00550: SGL_RS00550 - putative toxin-antitoxin system toxin component, PIN family, at 107,105 to 107,527
_RS00550
SGL_RS00555: SGL_RS00555 - toxin-antitoxin system HicB family antitoxin, at 107,531 to 107,746
_RS00555
SGL_RS00560: SGL_RS00560 - Rpn family recombination-promoting nuclease/putative transposase, at 107,767 to 108,585
_RS00560
SGL_RS00565: SGL_RS00565 - Rpn family recombination-promoting nuclease/putative transposase, at 108,733 to 109,503
_RS00565
Position (kb) 107
108
109 Strain fitness (log2 ratio) -1
0
1 at 106.795 kb on + strand, within SGL_RS00545 at 106.848 kb on + strand, within SGL_RS00545 at 106.913 kb on + strand at 106.915 kb on + strand at 107.120 kb on + strand at 107.249 kb on + strand, within SGL_RS00550 at 107.250 kb on - strand, within SGL_RS00550 at 107.293 kb on - strand, within SGL_RS00550 at 107.310 kb on + strand, within SGL_RS00550 at 107.312 kb on + strand, within SGL_RS00550 at 108.412 kb on + strand, within SGL_RS00560 at 109.138 kb on - strand, within SGL_RS00565 at 109.283 kb on + strand, within SGL_RS00565
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media supplemented with 5 .M CuSO4 but diluted to prevent light attenuation remove 106,795 + SGL_RS00545 0.62 +0.1 106,848 + SGL_RS00545 0.75 -0.0 106,913 + -0.2 106,915 + -0.5 107,120 + -0.5 107,249 + SGL_RS00550 0.34 +0.1 107,250 - SGL_RS00550 0.34 +0.6 107,293 - SGL_RS00550 0.44 +0.1 107,310 + SGL_RS00550 0.48 -0.1 107,312 + SGL_RS00550 0.49 -0.2 108,412 + SGL_RS00560 0.79 +0.8 109,138 - SGL_RS00565 0.53 +0.6 109,283 + SGL_RS00565 0.71 +0.0
Or see this region's nucleotide sequence