Experiment: growth in full media supplemented with 3 .M CuSO4 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14960 and SGL_RS14965 are separated by 310 nucleotides SGL_RS14965 and SGL_RS14970 are separated by 34 nucleotides SGL_RS14970 and SGL_RS14975 are separated by 94 nucleotides
SGL_RS14960: SGL_RS14960 - PHP domain-containing protein, at 2,809,055 to 2,809,804
_RS14960
SGL_RS14965: SGL_RS14965 - DUF2779 domain-containing protein, at 2,810,115 to 2,811,593
_RS14965
SGL_RS14970: SGL_RS14970 - hypothetical protein, at 2,811,628 to 2,812,290
_RS14970
SGL_RS14975: SGL_RS14975 - translation initiation factor, at 2,812,385 to 2,812,720
_RS14975
Position (kb)
2810
2811
2812 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2809.274 kb on + strand, within SGL_RS14960 at 2809.282 kb on + strand, within SGL_RS14960 at 2809.370 kb on + strand, within SGL_RS14960 at 2809.378 kb on - strand, within SGL_RS14960 at 2809.405 kb on - strand, within SGL_RS14960 at 2809.417 kb on + strand, within SGL_RS14960 at 2809.425 kb on - strand, within SGL_RS14960 at 2809.513 kb on - strand, within SGL_RS14960 at 2809.541 kb on - strand, within SGL_RS14960 at 2809.587 kb on + strand, within SGL_RS14960 at 2809.795 kb on - strand at 2809.854 kb on + strand at 2809.964 kb on - strand at 2810.088 kb on + strand at 2810.151 kb on + strand at 2810.159 kb on - strand at 2810.205 kb on + strand at 2810.346 kb on - strand, within SGL_RS14965 at 2810.353 kb on - strand, within SGL_RS14965 at 2810.416 kb on + strand, within SGL_RS14965 at 2810.516 kb on + strand, within SGL_RS14965 at 2810.590 kb on - strand, within SGL_RS14965 at 2810.653 kb on - strand, within SGL_RS14965 at 2810.738 kb on + strand, within SGL_RS14965 at 2811.026 kb on + strand, within SGL_RS14965 at 2811.125 kb on - strand, within SGL_RS14965 at 2811.154 kb on + strand, within SGL_RS14965 at 2811.172 kb on - strand, within SGL_RS14965 at 2811.252 kb on - strand, within SGL_RS14965 at 2811.436 kb on - strand, within SGL_RS14965 at 2811.466 kb on - strand at 2811.647 kb on + strand at 2811.648 kb on + strand at 2811.826 kb on + strand, within SGL_RS14970 at 2811.902 kb on + strand, within SGL_RS14970 at 2811.968 kb on + strand, within SGL_RS14970 at 2811.976 kb on - strand, within SGL_RS14970 at 2812.062 kb on + strand, within SGL_RS14970 at 2812.186 kb on + strand, within SGL_RS14970 at 2812.240 kb on - strand at 2812.506 kb on - strand, within SGL_RS14975
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media supplemented with 3 .M CuSO4 but diluted to prevent light attenuation remove 2,809,274 + SGL_RS14960 0.29 -1.0 2,809,282 + SGL_RS14960 0.30 -0.6 2,809,370 + SGL_RS14960 0.42 +0.8 2,809,378 - SGL_RS14960 0.43 -0.7 2,809,405 - SGL_RS14960 0.47 -0.3 2,809,417 + SGL_RS14960 0.48 -0.3 2,809,425 - SGL_RS14960 0.49 -1.2 2,809,513 - SGL_RS14960 0.61 -0.3 2,809,541 - SGL_RS14960 0.65 -0.4 2,809,587 + SGL_RS14960 0.71 -0.5 2,809,795 - -0.1 2,809,854 + -0.4 2,809,964 - -1.2 2,810,088 + -1.2 2,810,151 + -2.5 2,810,159 - -1.3 2,810,205 + -0.8 2,810,346 - SGL_RS14965 0.16 -1.3 2,810,353 - SGL_RS14965 0.16 -1.5 2,810,416 + SGL_RS14965 0.20 -1.7 2,810,516 + SGL_RS14965 0.27 -1.4 2,810,590 - SGL_RS14965 0.32 -1.2 2,810,653 - SGL_RS14965 0.36 -0.4 2,810,738 + SGL_RS14965 0.42 -1.6 2,811,026 + SGL_RS14965 0.62 -1.5 2,811,125 - SGL_RS14965 0.68 -2.1 2,811,154 + SGL_RS14965 0.70 -1.0 2,811,172 - SGL_RS14965 0.71 -1.1 2,811,252 - SGL_RS14965 0.77 -0.1 2,811,436 - SGL_RS14965 0.89 -1.1 2,811,466 - -1.7 2,811,647 + -0.8 2,811,648 + +1.1 2,811,826 + SGL_RS14970 0.30 +2.6 2,811,902 + SGL_RS14970 0.41 -0.2 2,811,968 + SGL_RS14970 0.51 +0.1 2,811,976 - SGL_RS14970 0.52 +1.3 2,812,062 + SGL_RS14970 0.65 +0.2 2,812,186 + SGL_RS14970 0.84 -0.3 2,812,240 - -0.1 2,812,506 - SGL_RS14975 0.36 +0.3
Or see this region's nucleotide sequence