Experiment: growth in full media supplemented with 3 .M CuSO4 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14315 and SGL_RS14320 are separated by 152 nucleotides SGL_RS14320 and SGL_RS14325 are separated by 20 nucleotides
SGL_RS14315: SGL_RS14315 - folate/biopterin family MFS transporter, at 2,664,403 to 2,665,887
_RS14315
SGL_RS14320: SGL_RS14320 - site-2 protease family protein, at 2,666,040 to 2,667,521
_RS14320
SGL_RS14325: SGL_RS14325 - ABC transporter substrate-binding protein, at 2,667,542 to 2,668,936
_RS14325
Position (kb)
2666
2667
2668 Strain fitness (log2 ratio)
-2
-1
0
1 at 2665.090 kb on - strand, within SGL_RS14315 at 2665.294 kb on - strand, within SGL_RS14315 at 2665.448 kb on + strand, within SGL_RS14315 at 2665.468 kb on + strand, within SGL_RS14315 at 2665.509 kb on + strand, within SGL_RS14315 at 2665.581 kb on - strand, within SGL_RS14315 at 2665.664 kb on + strand, within SGL_RS14315 at 2665.671 kb on + strand, within SGL_RS14315 at 2665.695 kb on - strand, within SGL_RS14315 at 2665.704 kb on + strand, within SGL_RS14315 at 2665.783 kb on + strand at 2665.783 kb on + strand at 2665.932 kb on - strand at 2666.023 kb on - strand at 2666.023 kb on - strand at 2666.251 kb on + strand, within SGL_RS14320 at 2666.421 kb on + strand, within SGL_RS14320 at 2666.423 kb on + strand, within SGL_RS14320 at 2666.423 kb on + strand, within SGL_RS14320 at 2666.431 kb on - strand, within SGL_RS14320 at 2666.679 kb on + strand, within SGL_RS14320 at 2666.762 kb on + strand, within SGL_RS14320 at 2667.022 kb on + strand, within SGL_RS14320 at 2667.027 kb on + strand, within SGL_RS14320 at 2667.035 kb on - strand, within SGL_RS14320 at 2667.035 kb on - strand, within SGL_RS14320 at 2667.039 kb on + strand, within SGL_RS14320 at 2667.159 kb on + strand, within SGL_RS14320 at 2667.167 kb on - strand, within SGL_RS14320 at 2667.222 kb on - strand, within SGL_RS14320 at 2667.339 kb on - strand, within SGL_RS14320 at 2667.453 kb on - strand at 2667.599 kb on - strand at 2667.619 kb on - strand at 2667.713 kb on + strand, within SGL_RS14325 at 2667.745 kb on - strand, within SGL_RS14325 at 2667.762 kb on - strand, within SGL_RS14325 at 2667.774 kb on + strand, within SGL_RS14325 at 2667.839 kb on - strand, within SGL_RS14325 at 2667.846 kb on - strand, within SGL_RS14325 at 2667.870 kb on - strand, within SGL_RS14325 at 2667.913 kb on + strand, within SGL_RS14325 at 2667.939 kb on - strand, within SGL_RS14325 at 2667.939 kb on - strand, within SGL_RS14325 at 2667.956 kb on + strand, within SGL_RS14325 at 2668.015 kb on - strand, within SGL_RS14325 at 2668.184 kb on + strand, within SGL_RS14325 at 2668.222 kb on - strand, within SGL_RS14325 at 2668.227 kb on + strand, within SGL_RS14325 at 2668.234 kb on - strand, within SGL_RS14325 at 2668.296 kb on + strand, within SGL_RS14325 at 2668.306 kb on + strand, within SGL_RS14325 at 2668.306 kb on + strand, within SGL_RS14325 at 2668.314 kb on - strand, within SGL_RS14325 at 2668.364 kb on - strand, within SGL_RS14325 at 2668.372 kb on - strand, within SGL_RS14325 at 2668.508 kb on + strand, within SGL_RS14325
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media supplemented with 3 .M CuSO4 but diluted to prevent light attenuation remove 2,665,090 - SGL_RS14315 0.46 +1.2 2,665,294 - SGL_RS14315 0.60 +0.8 2,665,448 + SGL_RS14315 0.70 +1.2 2,665,468 + SGL_RS14315 0.72 +1.1 2,665,509 + SGL_RS14315 0.74 +0.8 2,665,581 - SGL_RS14315 0.79 +0.6 2,665,664 + SGL_RS14315 0.85 +1.0 2,665,671 + SGL_RS14315 0.85 +0.9 2,665,695 - SGL_RS14315 0.87 +0.6 2,665,704 + SGL_RS14315 0.88 +0.6 2,665,783 + +1.7 2,665,783 + +0.8 2,665,932 - +0.0 2,666,023 - +0.0 2,666,023 - -0.3 2,666,251 + SGL_RS14320 0.14 -1.0 2,666,421 + SGL_RS14320 0.26 -1.6 2,666,423 + SGL_RS14320 0.26 -0.8 2,666,423 + SGL_RS14320 0.26 -1.6 2,666,431 - SGL_RS14320 0.26 -0.6 2,666,679 + SGL_RS14320 0.43 -0.5 2,666,762 + SGL_RS14320 0.49 -1.3 2,667,022 + SGL_RS14320 0.66 -1.9 2,667,027 + SGL_RS14320 0.67 -0.8 2,667,035 - SGL_RS14320 0.67 +0.3 2,667,035 - SGL_RS14320 0.67 -0.8 2,667,039 + SGL_RS14320 0.67 -1.0 2,667,159 + SGL_RS14320 0.76 -1.5 2,667,167 - SGL_RS14320 0.76 -2.6 2,667,222 - SGL_RS14320 0.80 -0.9 2,667,339 - SGL_RS14320 0.88 -0.9 2,667,453 - -0.8 2,667,599 - -0.6 2,667,619 - -0.1 2,667,713 + SGL_RS14325 0.12 +0.2 2,667,745 - SGL_RS14325 0.15 +0.5 2,667,762 - SGL_RS14325 0.16 -0.3 2,667,774 + SGL_RS14325 0.17 -0.3 2,667,839 - SGL_RS14325 0.21 +0.5 2,667,846 - SGL_RS14325 0.22 -0.4 2,667,870 - SGL_RS14325 0.24 +0.3 2,667,913 + SGL_RS14325 0.27 +0.3 2,667,939 - SGL_RS14325 0.28 -0.1 2,667,939 - SGL_RS14325 0.28 -1.0 2,667,956 + SGL_RS14325 0.30 -0.6 2,668,015 - SGL_RS14325 0.34 -0.1 2,668,184 + SGL_RS14325 0.46 +0.4 2,668,222 - SGL_RS14325 0.49 -1.4 2,668,227 + SGL_RS14325 0.49 +0.4 2,668,234 - SGL_RS14325 0.50 -0.1 2,668,296 + SGL_RS14325 0.54 -0.4 2,668,306 + SGL_RS14325 0.55 -0.8 2,668,306 + SGL_RS14325 0.55 +0.2 2,668,314 - SGL_RS14325 0.55 +0.3 2,668,364 - SGL_RS14325 0.59 -0.6 2,668,372 - SGL_RS14325 0.59 -0.3 2,668,508 + SGL_RS14325 0.69 -0.1
Or see this region's nucleotide sequence