Experiment: growth in full media supplemented with 3 .M CuSO4 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS07035 and SGL_RS07040 are separated by 187 nucleotides SGL_RS07040 and SGL_RS07045 are separated by 382 nucleotides SGL_RS07045 and SGL_RS07050 are separated by 120 nucleotides
SGL_RS07035: SGL_RS07035 - pyruvate kinase, at 1,102,283 to 1,104,058
_RS07035
SGL_RS07040: SGL_RS07040 - DUF1824 family protein, at 1,104,246 to 1,104,647
_RS07040
SGL_RS07045: SGL_RS07045 - TIGR02652 family protein, at 1,105,030 to 1,105,545
_RS07045
SGL_RS07050: SGL_RS07050 - polyphosphate:AMP phosphotransferase, at 1,105,666 to 1,107,168
_RS07050
Position (kb)
1105
1106 Strain fitness (log2 ratio)
-1
0
1 at 1104.100 kb on - strand at 1104.179 kb on + strand at 1104.194 kb on - strand at 1104.278 kb on + strand at 1104.298 kb on + strand, within SGL_RS07040 at 1104.338 kb on - strand, within SGL_RS07040 at 1104.398 kb on + strand, within SGL_RS07040 at 1104.406 kb on - strand, within SGL_RS07040 at 1104.474 kb on - strand, within SGL_RS07040 at 1104.591 kb on + strand, within SGL_RS07040 at 1104.860 kb on + strand at 1105.129 kb on - strand, within SGL_RS07045 at 1105.151 kb on + strand, within SGL_RS07045 at 1105.164 kb on + strand, within SGL_RS07045 at 1105.182 kb on - strand, within SGL_RS07045 at 1105.220 kb on + strand, within SGL_RS07045 at 1105.275 kb on + strand, within SGL_RS07045 at 1105.280 kb on + strand, within SGL_RS07045 at 1105.288 kb on - strand, within SGL_RS07045 at 1105.291 kb on + strand, within SGL_RS07045 at 1105.291 kb on + strand, within SGL_RS07045 at 1105.454 kb on - strand, within SGL_RS07045 at 1105.462 kb on + strand, within SGL_RS07045 at 1105.633 kb on - strand at 1105.661 kb on + strand at 1105.674 kb on - strand at 1105.684 kb on - strand at 1105.751 kb on + strand at 1105.751 kb on + strand at 1105.751 kb on + strand at 1105.751 kb on + strand at 1105.759 kb on - strand at 1105.764 kb on - strand at 1106.091 kb on - strand, within SGL_RS07050 at 1106.292 kb on + strand, within SGL_RS07050 at 1106.319 kb on - strand, within SGL_RS07050 at 1106.329 kb on + strand, within SGL_RS07050 at 1106.362 kb on + strand, within SGL_RS07050
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media supplemented with 3 .M CuSO4 but diluted to prevent light attenuation remove 1,104,100 - -0.9 1,104,179 + -0.6 1,104,194 - -0.3 1,104,278 + -0.7 1,104,298 + SGL_RS07040 0.13 -0.9 1,104,338 - SGL_RS07040 0.23 -0.4 1,104,398 + SGL_RS07040 0.38 -0.1 1,104,406 - SGL_RS07040 0.40 -0.1 1,104,474 - SGL_RS07040 0.57 -0.5 1,104,591 + SGL_RS07040 0.86 -0.9 1,104,860 + -0.3 1,105,129 - SGL_RS07045 0.19 +0.5 1,105,151 + SGL_RS07045 0.23 -1.6 1,105,164 + SGL_RS07045 0.26 -0.5 1,105,182 - SGL_RS07045 0.29 -1.0 1,105,220 + SGL_RS07045 0.37 -1.6 1,105,275 + SGL_RS07045 0.47 -0.8 1,105,280 + SGL_RS07045 0.48 -1.2 1,105,288 - SGL_RS07045 0.50 -0.6 1,105,291 + SGL_RS07045 0.51 -1.0 1,105,291 + SGL_RS07045 0.51 -1.5 1,105,454 - SGL_RS07045 0.82 -1.3 1,105,462 + SGL_RS07045 0.84 -0.7 1,105,633 - +0.1 1,105,661 + +0.4 1,105,674 - +0.7 1,105,684 - -0.3 1,105,751 + -0.2 1,105,751 + -0.2 1,105,751 + -0.0 1,105,751 + +0.1 1,105,759 - -0.4 1,105,764 - +0.1 1,106,091 - SGL_RS07050 0.28 -0.1 1,106,292 + SGL_RS07050 0.42 +0.4 1,106,319 - SGL_RS07050 0.43 -0.1 1,106,329 + SGL_RS07050 0.44 -1.3 1,106,362 + SGL_RS07050 0.46 +0.2
Or see this region's nucleotide sequence