Experiment: growth in full media supplemented with 3 .M CuSO4 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS00105 and SGL_RS00110 are separated by 38 nucleotides SGL_RS00110 and SGL_RS00115 overlap by 1 nucleotides SGL_RS00115 and SGL_RS00120 are separated by 123 nucleotides SGL_RS00120 and SGL_RS00125 are separated by 42 nucleotides
SGL_RS00105: SGL_RS00105 - cysteine desulfurase family protein, at 24,798 to 25,955
_RS00105
SGL_RS00110: SGL_RS00110 - AAA family ATPase, at 25,994 to 27,175
_RS00110
SGL_RS00115: SGL_RS00115 - DUF4276 family protein, at 27,175 to 27,735
_RS00115
SGL_RS00120: SGL_RS00120 - DUF433 domain-containing protein, at 27,859 to 28,053
_RS00120
SGL_RS00125: SGL_RS00125 - DNA phosphorothioation-associated putative methyltransferase, at 28,096 to 30,168
_RS00125
Position (kb)
25
26
27
28 Strain fitness (log2 ratio)
-1
0
1 at 25.023 kb on - strand, within SGL_RS00105 at 25.041 kb on + strand, within SGL_RS00105 at 25.118 kb on - strand, within SGL_RS00105 at 25.118 kb on - strand, within SGL_RS00105 at 25.222 kb on + strand, within SGL_RS00105 at 25.222 kb on + strand, within SGL_RS00105 at 25.222 kb on + strand, within SGL_RS00105 at 25.222 kb on + strand, within SGL_RS00105 at 25.230 kb on - strand, within SGL_RS00105 at 25.230 kb on - strand, within SGL_RS00105 at 25.230 kb on - strand, within SGL_RS00105 at 25.232 kb on + strand, within SGL_RS00105 at 25.240 kb on - strand, within SGL_RS00105 at 25.246 kb on - strand, within SGL_RS00105 at 25.379 kb on - strand, within SGL_RS00105 at 25.402 kb on - strand, within SGL_RS00105 at 25.521 kb on - strand, within SGL_RS00105 at 25.532 kb on + strand, within SGL_RS00105 at 25.539 kb on - strand, within SGL_RS00105 at 25.539 kb on - strand, within SGL_RS00105 at 25.541 kb on + strand, within SGL_RS00105 at 25.549 kb on - strand, within SGL_RS00105 at 25.662 kb on + strand, within SGL_RS00105 at 25.681 kb on - strand, within SGL_RS00105 at 25.694 kb on - strand, within SGL_RS00105 at 25.718 kb on - strand, within SGL_RS00105 at 25.798 kb on + strand, within SGL_RS00105 at 25.813 kb on - strand, within SGL_RS00105 at 26.888 kb on - strand, within SGL_RS00110 at 26.965 kb on + strand, within SGL_RS00110 at 27.023 kb on + strand, within SGL_RS00110 at 27.137 kb on + strand at 27.738 kb on + strand at 28.000 kb on - strand, within SGL_RS00120 at 28.000 kb on - strand, within SGL_RS00120 at 28.035 kb on + strand at 28.043 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media supplemented with 3 .M CuSO4 but diluted to prevent light attenuation remove 25,023 - SGL_RS00105 0.19 -0.4 25,041 + SGL_RS00105 0.21 +0.2 25,118 - SGL_RS00105 0.28 +0.5 25,118 - SGL_RS00105 0.28 -1.0 25,222 + SGL_RS00105 0.37 +0.2 25,222 + SGL_RS00105 0.37 -0.5 25,222 + SGL_RS00105 0.37 +0.2 25,222 + SGL_RS00105 0.37 +0.3 25,230 - SGL_RS00105 0.37 +0.0 25,230 - SGL_RS00105 0.37 -0.1 25,230 - SGL_RS00105 0.37 -0.1 25,232 + SGL_RS00105 0.37 -0.7 25,240 - SGL_RS00105 0.38 +0.3 25,246 - SGL_RS00105 0.39 +0.1 25,379 - SGL_RS00105 0.50 -0.3 25,402 - SGL_RS00105 0.52 -0.2 25,521 - SGL_RS00105 0.62 +0.2 25,532 + SGL_RS00105 0.63 -0.0 25,539 - SGL_RS00105 0.64 -0.1 25,539 - SGL_RS00105 0.64 +0.4 25,541 + SGL_RS00105 0.64 +0.1 25,549 - SGL_RS00105 0.65 +0.5 25,662 + SGL_RS00105 0.75 +0.7 25,681 - SGL_RS00105 0.76 +1.3 25,694 - SGL_RS00105 0.77 -0.1 25,718 - SGL_RS00105 0.79 -0.2 25,798 + SGL_RS00105 0.86 +0.1 25,813 - SGL_RS00105 0.88 -0.1 26,888 - SGL_RS00110 0.76 -1.5 26,965 + SGL_RS00110 0.82 -0.5 27,023 + SGL_RS00110 0.87 -0.8 27,137 + -1.3 27,738 + -1.2 28,000 - SGL_RS00120 0.72 -0.6 28,000 - SGL_RS00120 0.72 -0.4 28,035 + +0.0 28,043 - -0.3
Or see this region's nucleotide sequence