Experiment: growth in full media but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12270 and SGL_RS12275 are separated by 349 nucleotides SGL_RS12275 and SGL_RS12280 are separated by 79 nucleotides SGL_RS12280 and SGL_RS12285 are separated by 114 nucleotides SGL_RS12285 and SGL_RS12290 overlap by 8 nucleotides
SGL_RS12270: SGL_RS12270 - peptide chain release factor 2, at 2,214,685 to 2,215,801
_RS12270
SGL_RS12275: SGL_RS12275 - hypothetical protein, at 2,216,151 to 2,216,396
_RS12275
SGL_RS12280: SGL_RS12280 - chloride channel protein, at 2,216,476 to 2,219,175
_RS12280
SGL_RS12285: SGL_RS12285 - hypothetical protein, at 2,219,290 to 2,219,793
_RS12285
SGL_RS12290: SGL_RS12290 - DUF58 domain-containing protein, at 2,219,786 to 2,220,961
_RS12290
Position (kb)
2216
2217
2218
2219
2220 Strain fitness (log2 ratio)
-1
0
1 at 2215.855 kb on + strand at 2215.873 kb on - strand at 2216.232 kb on - strand, within SGL_RS12275 at 2216.238 kb on + strand, within SGL_RS12275 at 2216.538 kb on + strand at 2216.944 kb on - strand, within SGL_RS12280 at 2216.990 kb on - strand, within SGL_RS12280 at 2217.173 kb on + strand, within SGL_RS12280 at 2217.411 kb on - strand, within SGL_RS12280 at 2217.599 kb on - strand, within SGL_RS12280 at 2217.604 kb on - strand, within SGL_RS12280 at 2217.614 kb on - strand, within SGL_RS12280 at 2217.646 kb on + strand, within SGL_RS12280 at 2217.661 kb on + strand, within SGL_RS12280 at 2217.762 kb on + strand, within SGL_RS12280 at 2217.903 kb on + strand, within SGL_RS12280 at 2218.114 kb on + strand, within SGL_RS12280 at 2218.117 kb on - strand, within SGL_RS12280 at 2218.122 kb on - strand, within SGL_RS12280 at 2218.185 kb on - strand, within SGL_RS12280 at 2218.315 kb on + strand, within SGL_RS12280 at 2218.324 kb on + strand, within SGL_RS12280 at 2218.386 kb on + strand, within SGL_RS12280 at 2218.389 kb on - strand, within SGL_RS12280 at 2218.544 kb on - strand, within SGL_RS12280 at 2218.582 kb on + strand, within SGL_RS12280 at 2218.588 kb on - strand, within SGL_RS12280 at 2218.589 kb on - strand, within SGL_RS12280 at 2218.658 kb on - strand, within SGL_RS12280 at 2218.732 kb on + strand, within SGL_RS12280 at 2218.747 kb on + strand, within SGL_RS12280 at 2218.797 kb on - strand, within SGL_RS12280 at 2218.803 kb on - strand, within SGL_RS12280 at 2218.927 kb on - strand at 2219.010 kb on + strand at 2220.122 kb on + strand, within SGL_RS12290 at 2220.133 kb on + strand, within SGL_RS12290 at 2220.133 kb on + strand, within SGL_RS12290 at 2220.133 kb on + strand, within SGL_RS12290
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media but diluted to prevent light attenuation remove 2,215,855 + +0.3 2,215,873 - +0.4 2,216,232 - SGL_RS12275 0.33 -0.3 2,216,238 + SGL_RS12275 0.35 -0.0 2,216,538 + -0.3 2,216,944 - SGL_RS12280 0.17 +0.9 2,216,990 - SGL_RS12280 0.19 -0.3 2,217,173 + SGL_RS12280 0.26 +0.0 2,217,411 - SGL_RS12280 0.35 +0.2 2,217,599 - SGL_RS12280 0.42 +0.3 2,217,604 - SGL_RS12280 0.42 +0.2 2,217,614 - SGL_RS12280 0.42 +0.3 2,217,646 + SGL_RS12280 0.43 +0.3 2,217,661 + SGL_RS12280 0.44 -0.3 2,217,762 + SGL_RS12280 0.48 +0.7 2,217,903 + SGL_RS12280 0.53 +0.3 2,218,114 + SGL_RS12280 0.61 -0.0 2,218,117 - SGL_RS12280 0.61 -0.2 2,218,122 - SGL_RS12280 0.61 +0.1 2,218,185 - SGL_RS12280 0.63 -0.2 2,218,315 + SGL_RS12280 0.68 +0.6 2,218,324 + SGL_RS12280 0.68 +0.3 2,218,386 + SGL_RS12280 0.71 +0.3 2,218,389 - SGL_RS12280 0.71 -0.4 2,218,544 - SGL_RS12280 0.77 -0.1 2,218,582 + SGL_RS12280 0.78 -0.0 2,218,588 - SGL_RS12280 0.78 +0.1 2,218,589 - SGL_RS12280 0.78 +0.2 2,218,658 - SGL_RS12280 0.81 +0.0 2,218,732 + SGL_RS12280 0.84 -0.3 2,218,747 + SGL_RS12280 0.84 +0.6 2,218,797 - SGL_RS12280 0.86 +1.0 2,218,803 - SGL_RS12280 0.86 -1.0 2,218,927 - +0.1 2,219,010 + -0.3 2,220,122 + SGL_RS12290 0.29 -0.0 2,220,133 + SGL_RS12290 0.30 +0.2 2,220,133 + SGL_RS12290 0.30 +0.4 2,220,133 + SGL_RS12290 0.30 -0.2
Or see this region's nucleotide sequence