Experiment: growth in full media bubbled with ambient air
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS13795 and SGL_RS13800 are separated by 62 nucleotides SGL_RS13800 and SGL_RS13805 are separated by 93 nucleotides SGL_RS13805 and SGL_RS13810 are separated by 25 nucleotides SGL_RS13810 and SGL_RS13815 are separated by 74 nucleotides
SGL_RS13795: SGL_RS13795 - phosphohistidine phosphatase SixA, at 2,561,499 to 2,561,993
_RS13795
SGL_RS13800: SGL_RS13800 - alpha/beta hydrolase, at 2,562,056 to 2,562,859
_RS13800
SGL_RS13805: SGL_RS13805 - saccharopine dehydrogenase family protein, at 2,562,953 to 2,564,149
_RS13805
SGL_RS13810: SGL_RS13810 - sulfur carrier protein ThiS, at 2,564,175 to 2,564,390
_RS13810
SGL_RS13815: SGL_RS13815 - ribonuclease Z, at 2,564,465 to 2,565,445
_RS13815
Position (kb)
2562
2563
2564
2565 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 2561.954 kb on + strand at 2562.258 kb on - strand, within SGL_RS13800 at 2562.345 kb on + strand, within SGL_RS13800 at 2562.495 kb on + strand, within SGL_RS13800 at 2562.796 kb on + strand at 2562.828 kb on - strand at 2562.867 kb on + strand at 2562.877 kb on + strand at 2562.877 kb on + strand at 2562.885 kb on - strand at 2562.926 kb on + strand at 2563.068 kb on - strand at 2563.068 kb on - strand at 2563.170 kb on - strand, within SGL_RS13805 at 2563.190 kb on + strand, within SGL_RS13805 at 2563.349 kb on + strand, within SGL_RS13805 at 2563.444 kb on + strand, within SGL_RS13805 at 2563.602 kb on - strand, within SGL_RS13805 at 2563.819 kb on - strand, within SGL_RS13805 at 2563.977 kb on + strand, within SGL_RS13805 at 2564.039 kb on + strand at 2564.407 kb on + strand at 2564.407 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air remove 2,561,954 + +0.3 2,562,258 - SGL_RS13800 0.25 -0.1 2,562,345 + SGL_RS13800 0.36 -0.1 2,562,495 + SGL_RS13800 0.55 +0.3 2,562,796 + +1.0 2,562,828 - +0.5 2,562,867 + -1.1 2,562,877 + +0.9 2,562,877 + +0.1 2,562,885 - +0.1 2,562,926 + +0.1 2,563,068 - +2.7 2,563,068 - +3.2 2,563,170 - SGL_RS13805 0.18 +3.5 2,563,190 + SGL_RS13805 0.20 +2.9 2,563,349 + SGL_RS13805 0.33 +2.2 2,563,444 + SGL_RS13805 0.41 +3.8 2,563,602 - SGL_RS13805 0.54 +2.7 2,563,819 - SGL_RS13805 0.72 +3.8 2,563,977 + SGL_RS13805 0.86 +3.6 2,564,039 + +4.3 2,564,407 + -0.3 2,564,407 + -0.7
Or see this region's nucleotide sequence