Experiment: growth in full media bubbled with ambient air
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03485 and SGL_RS03490 are separated by 303 nucleotides SGL_RS03490 and SGL_RS03495 are separated by 23 nucleotides SGL_RS03495 and SGL_RS03500 are separated by 99 nucleotides SGL_RS03500 and SGL_RS03505 are separated by 65 nucleotides
SGL_RS03485: SGL_RS03485 - translation initiation factor IF-3, at 343,026 to 343,559
_RS03485
SGL_RS03490: SGL_RS03490 - YaaW family protein, at 343,863 to 344,720
_RS03490
SGL_RS03495: SGL_RS03495 - metal-binding protein, at 344,744 to 345,298
_RS03495
SGL_RS03500: SGL_RS03500 - tRNA-Val, at 345,398 to 345,470
_RS03500
SGL_RS03505: SGL_RS03505 - exopolysaccharide transport family protein, at 345,536 to 347,806
_RS03505
Position (kb) 343
344
345 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 343.562 kb on - strand at 344.079 kb on - strand, within SGL_RS03490 at 344.095 kb on - strand, within SGL_RS03490 at 344.254 kb on - strand, within SGL_RS03490 at 344.273 kb on - strand, within SGL_RS03490 at 344.385 kb on + strand, within SGL_RS03490 at 344.597 kb on - strand, within SGL_RS03490 at 344.613 kb on + strand, within SGL_RS03490 at 344.684 kb on - strand at 344.842 kb on + strand, within SGL_RS03495 at 344.850 kb on - strand, within SGL_RS03495 at 344.913 kb on - strand, within SGL_RS03495 at 344.965 kb on + strand, within SGL_RS03495 at 345.178 kb on + strand, within SGL_RS03495 at 345.265 kb on + strand at 345.305 kb on + strand at 345.605 kb on + strand at 345.605 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media bubbled with ambient air remove 343,562 - -0.6 344,079 - SGL_RS03490 0.25 +2.1 344,095 - SGL_RS03490 0.27 +1.7 344,254 - SGL_RS03490 0.46 +1.8 344,273 - SGL_RS03490 0.48 +0.9 344,385 + SGL_RS03490 0.61 +1.6 344,597 - SGL_RS03490 0.86 +0.9 344,613 + SGL_RS03490 0.87 +1.7 344,684 - +1.5 344,842 + SGL_RS03495 0.18 -2.8 344,850 - SGL_RS03495 0.19 -0.8 344,913 - SGL_RS03495 0.30 -1.2 344,965 + SGL_RS03495 0.40 -3.7 345,178 + SGL_RS03495 0.78 -0.8 345,265 + -0.3 345,305 + +1.0 345,605 + +0.2 345,605 - -0.2
Or see this region's nucleotide sequence