Experiment: growth in media lacking HCO3 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS05890 and SGL_RS05895 are separated by 155 nucleotides SGL_RS05895 and SGL_RS05900 are separated by 59 nucleotides SGL_RS05900 and SGL_RS18720 are separated by 131 nucleotides SGL_RS18720 and SGL_RS18725 are separated by 22 nucleotides
SGL_RS05890: SGL_RS05890 - urease accessory protein UreF, at 849,095 to 849,748
_RS05890
SGL_RS05895: SGL_RS05895 - DUF3386 domain-containing protein, at 849,904 to 850,545
_RS05895
SGL_RS05900: SGL_RS05900 - ATP-binding cassette domain-containing protein, at 850,605 to 851,585
_RS05900
SGL_RS18720: SGL_RS18720 - IS1 family transposase, at 851,717 to 852,447
_RS18720
SGL_RS18725: SGL_RS18725 - IS5-like element IS5Sa2 family transposase, at 852,470 to 853,281
_RS18725
Position (kb) 850
851
852 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 849.683 kb on + strand at 849.684 kb on - strand at 849.902 kb on - strand at 850.034 kb on + strand, within SGL_RS05895 at 850.050 kb on + strand, within SGL_RS05895 at 850.098 kb on - strand, within SGL_RS05895 at 850.124 kb on + strand, within SGL_RS05895 at 850.124 kb on + strand, within SGL_RS05895 at 850.214 kb on + strand, within SGL_RS05895 at 850.227 kb on + strand, within SGL_RS05895 at 850.391 kb on - strand, within SGL_RS05895 at 850.407 kb on - strand, within SGL_RS05895 at 850.435 kb on - strand, within SGL_RS05895 at 850.462 kb on + strand, within SGL_RS05895 at 850.754 kb on - strand, within SGL_RS05900 at 851.100 kb on + strand, within SGL_RS05900 at 851.265 kb on - strand, within SGL_RS05900 at 851.341 kb on - strand, within SGL_RS05900 at 851.417 kb on - strand, within SGL_RS05900 at 851.451 kb on + strand, within SGL_RS05900 at 851.800 kb on + strand, within SGL_RS18720 at 851.884 kb on - strand, within SGL_RS18720 at 851.884 kb on - strand, within SGL_RS18720
Per-strain Table
Position Strand Gene LocusTag Fraction growth in media lacking HCO3 but diluted to prevent light attenuation remove 849,683 + +2.9 849,684 - -0.1 849,902 - -2.9 850,034 + SGL_RS05895 0.20 -2.2 850,050 + SGL_RS05895 0.23 -3.3 850,098 - SGL_RS05895 0.30 -3.1 850,124 + SGL_RS05895 0.34 -0.9 850,124 + SGL_RS05895 0.34 -2.3 850,214 + SGL_RS05895 0.48 -1.5 850,227 + SGL_RS05895 0.50 -4.1 850,391 - SGL_RS05895 0.76 -1.4 850,407 - SGL_RS05895 0.78 -3.1 850,435 - SGL_RS05895 0.83 -5.3 850,462 + SGL_RS05895 0.87 -1.8 850,754 - SGL_RS05900 0.15 -2.9 851,100 + SGL_RS05900 0.50 -3.0 851,265 - SGL_RS05900 0.67 -2.8 851,341 - SGL_RS05900 0.75 -4.6 851,417 - SGL_RS05900 0.83 -2.1 851,451 + SGL_RS05900 0.86 -2.2 851,800 + SGL_RS18720 0.11 +0.3 851,884 - SGL_RS18720 0.23 +0.2 851,884 - SGL_RS18720 0.23 -0.2
Or see this region's nucleotide sequence