Experiment: growth in media lacking HCO3 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS00600 and SGL_RS00605 are separated by 218 nucleotides SGL_RS00605 and SGL_RS00610 are separated by 95 nucleotides SGL_RS00610 and SGL_RS00615 are separated by 55 nucleotides SGL_RS00615 and SGL_RS00620 are separated by 51 nucleotides
SGL_RS00600: SGL_RS00600 - hypothetical protein, at 114,514 to 115,284
_RS00600
SGL_RS00605: SGL_RS00605 - hypothetical protein, at 115,503 to 116,540
_RS00605
SGL_RS00610: SGL_RS00610 - hypothetical protein, at 116,636 to 116,830
_RS00610
SGL_RS00615: SGL_RS00615 - DUF1524 domain-containing protein, at 116,886 to 117,293
_RS00615
SGL_RS00620: SGL_RS00620 - IS4-like element IS4Sa family transposase, at 117,345 to 118,361
_RS00620
Position (kb) 115
116
117 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 114.692 kb on - strand, within SGL_RS00600 at 115.255 kb on + strand at 115.366 kb on + strand at 116.246 kb on + strand, within SGL_RS00605 at 116.263 kb on + strand, within SGL_RS00605 at 116.295 kb on + strand, within SGL_RS00605 at 116.647 kb on + strand at 117.028 kb on - strand, within SGL_RS00615 at 117.126 kb on + strand, within SGL_RS00615 at 117.134 kb on - strand, within SGL_RS00615 at 117.135 kb on - strand, within SGL_RS00615 at 117.140 kb on + strand, within SGL_RS00615 at 117.146 kb on - strand, within SGL_RS00615
Per-strain Table
Position Strand Gene LocusTag Fraction growth in media lacking HCO3 but diluted to prevent light attenuation remove 114,692 - SGL_RS00600 0.23 -1.1 115,255 + -0.5 115,366 + +0.0 116,246 + SGL_RS00605 0.72 -2.4 116,263 + SGL_RS00605 0.73 -2.5 116,295 + SGL_RS00605 0.76 -2.1 116,647 + -0.6 117,028 - SGL_RS00615 0.35 -2.2 117,126 + SGL_RS00615 0.59 -1.1 117,134 - SGL_RS00615 0.61 +0.3 117,135 - SGL_RS00615 0.61 -0.6 117,140 + SGL_RS00615 0.62 -2.4 117,146 - SGL_RS00615 0.64 -3.4
Or see this region's nucleotide sequence