Experiment: growth in media lacking HCO3 buffered with 10 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04900 and SGL_RS04905 are separated by 28 nucleotides SGL_RS04905 and SGL_RS19435 are separated by 97 nucleotides SGL_RS19435 and SGL_RS04910 are separated by 295 nucleotides SGL_RS04910 and SGL_RS04915 are separated by 214 nucleotides
SGL_RS04900: SGL_RS04900 - S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase, at 644,388 to 645,497
_RS04900
SGL_RS04905: SGL_RS04905 - tRNA-Arg, at 645,526 to 645,598
_RS04905
SGL_RS19435: SGL_RS19435 - hypothetical protein, at 645,696 to 645,833
_RS19435
SGL_RS04910: SGL_RS04910 - SwmB domain-containing protein, at 646,129 to 658,047
_RS04910
SGL_RS04915: SGL_RS04915 - magnesium chelatase ATPase subunit I, at 658,262 to 659,335
_RS04915
Position (kb) 646
647
648
649
650
651
652
653
654
655
656
657
658
659 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 645.156 kb on + strand, within SGL_RS04900 at 645.161 kb on + strand, within SGL_RS04900 at 645.173 kb on - strand, within SGL_RS04900 at 645.186 kb on - strand, within SGL_RS04900 at 645.232 kb on + strand, within SGL_RS04900 at 645.278 kb on - strand, within SGL_RS04900 at 645.315 kb on + strand, within SGL_RS04900 at 645.323 kb on - strand, within SGL_RS04900 at 645.367 kb on - strand, within SGL_RS04900 at 645.691 kb on - strand at 645.701 kb on + strand at 645.724 kb on - strand, within SGL_RS19435 at 645.966 kb on - strand at 645.968 kb on + strand at 646.007 kb on + strand at 646.165 kb on + strand at 646.173 kb on - strand at 646.173 kb on - strand at 646.173 kb on - strand at 646.228 kb on + strand at 646.236 kb on - strand at 646.401 kb on - strand at 646.479 kb on - strand at 646.611 kb on - strand at 646.672 kb on - strand at 646.761 kb on + strand at 646.761 kb on + strand at 646.780 kb on + strand at 646.840 kb on - strand at 646.869 kb on - strand at 647.223 kb on + strand at 647.254 kb on - strand at 647.334 kb on - strand, within SGL_RS04910 at 647.369 kb on - strand, within SGL_RS04910 at 647.392 kb on + strand, within SGL_RS04910 at 647.425 kb on + strand, within SGL_RS04910 at 647.434 kb on + strand, within SGL_RS04910 at 647.459 kb on - strand, within SGL_RS04910 at 647.822 kb on + strand, within SGL_RS04910 at 647.860 kb on - strand, within SGL_RS04910 at 647.895 kb on - strand, within SGL_RS04910 at 647.951 kb on - strand, within SGL_RS04910 at 647.975 kb on + strand, within SGL_RS04910 at 647.980 kb on + strand, within SGL_RS04910 at 647.985 kb on + strand, within SGL_RS04910 at 647.985 kb on + strand, within SGL_RS04910 at 647.988 kb on - strand, within SGL_RS04910 at 647.993 kb on - strand, within SGL_RS04910 at 648.145 kb on + strand, within SGL_RS04910 at 648.145 kb on + strand, within SGL_RS04910 at 648.209 kb on - strand, within SGL_RS04910 at 648.573 kb on + strand, within SGL_RS04910 at 649.336 kb on + strand, within SGL_RS04910 at 649.543 kb on + strand, within SGL_RS04910 at 649.546 kb on + strand, within SGL_RS04910 at 649.628 kb on + strand, within SGL_RS04910 at 649.695 kb on - strand, within SGL_RS04910 at 649.745 kb on - strand, within SGL_RS04910 at 649.780 kb on - strand, within SGL_RS04910 at 649.814 kb on + strand, within SGL_RS04910 at 650.010 kb on + strand, within SGL_RS04910 at 650.082 kb on + strand, within SGL_RS04910 at 650.115 kb on + strand, within SGL_RS04910 at 650.123 kb on - strand, within SGL_RS04910 at 650.123 kb on - strand, within SGL_RS04910 at 650.152 kb on - strand, within SGL_RS04910 at 650.293 kb on + strand, within SGL_RS04910 at 650.341 kb on + strand, within SGL_RS04910 at 650.426 kb on - strand, within SGL_RS04910 at 650.740 kb on - strand, within SGL_RS04910 at 650.960 kb on - strand, within SGL_RS04910 at 651.028 kb on + strand, within SGL_RS04910 at 651.036 kb on - strand, within SGL_RS04910 at 651.038 kb on + strand, within SGL_RS04910 at 651.046 kb on - strand, within SGL_RS04910 at 651.073 kb on + strand, within SGL_RS04910 at 651.435 kb on - strand, within SGL_RS04910 at 651.722 kb on + strand, within SGL_RS04910 at 651.749 kb on - strand, within SGL_RS04910 at 651.778 kb on + strand, within SGL_RS04910 at 651.916 kb on + strand, within SGL_RS04910 at 651.916 kb on + strand, within SGL_RS04910 at 652.007 kb on + strand, within SGL_RS04910 at 652.021 kb on - strand, within SGL_RS04910 at 652.067 kb on + strand, within SGL_RS04910 at 652.269 kb on + strand, within SGL_RS04910 at 652.350 kb on - strand, within SGL_RS04910 at 652.547 kb on + strand, within SGL_RS04910 at 652.558 kb on + strand, within SGL_RS04910 at 652.648 kb on - strand, within SGL_RS04910 at 652.702 kb on + strand, within SGL_RS04910 at 652.748 kb on + strand, within SGL_RS04910 at 652.774 kb on + strand, within SGL_RS04910 at 652.859 kb on - strand, within SGL_RS04910 at 652.885 kb on + strand, within SGL_RS04910 at 652.921 kb on + strand, within SGL_RS04910 at 653.325 kb on + strand, within SGL_RS04910 at 653.373 kb on + strand, within SGL_RS04910 at 653.463 kb on + strand, within SGL_RS04910 at 653.533 kb on + strand, within SGL_RS04910 at 653.596 kb on + strand, within SGL_RS04910 at 653.727 kb on + strand, within SGL_RS04910 at 653.808 kb on + strand, within SGL_RS04910 at 654.015 kb on + strand, within SGL_RS04910 at 654.021 kb on + strand, within SGL_RS04910 at 654.074 kb on + strand, within SGL_RS04910 at 654.136 kb on - strand, within SGL_RS04910 at 654.140 kb on + strand, within SGL_RS04910 at 654.146 kb on - strand, within SGL_RS04910 at 654.150 kb on + strand, within SGL_RS04910 at 654.158 kb on - strand, within SGL_RS04910 at 654.215 kb on + strand, within SGL_RS04910 at 654.222 kb on - strand, within SGL_RS04910 at 654.447 kb on - strand, within SGL_RS04910 at 654.467 kb on + strand, within SGL_RS04910 at 654.470 kb on - strand, within SGL_RS04910 at 654.682 kb on - strand, within SGL_RS04910 at 654.704 kb on + strand, within SGL_RS04910 at 654.707 kb on + strand, within SGL_RS04910 at 654.837 kb on - strand, within SGL_RS04910 at 654.968 kb on + strand, within SGL_RS04910 at 655.185 kb on - strand, within SGL_RS04910 at 655.189 kb on + strand, within SGL_RS04910 at 655.409 kb on + strand, within SGL_RS04910 at 655.586 kb on - strand, within SGL_RS04910 at 655.659 kb on - strand, within SGL_RS04910 at 655.735 kb on + strand, within SGL_RS04910 at 656.225 kb on + strand, within SGL_RS04910 at 656.238 kb on - strand, within SGL_RS04910 at 656.256 kb on + strand, within SGL_RS04910 at 656.267 kb on + strand, within SGL_RS04910 at 656.275 kb on - strand, within SGL_RS04910 at 656.299 kb on - strand, within SGL_RS04910 at 656.311 kb on + strand, within SGL_RS04910 at 656.402 kb on + strand, within SGL_RS04910 at 656.430 kb on - strand, within SGL_RS04910 at 656.523 kb on + strand, within SGL_RS04910 at 656.713 kb on + strand, within SGL_RS04910 at 656.769 kb on - strand, within SGL_RS04910 at 656.928 kb on - strand at 656.965 kb on - strand at 657.093 kb on + strand at 657.135 kb on - strand at 657.226 kb on - strand at 657.286 kb on + strand at 657.286 kb on + strand at 657.288 kb on - strand at 657.622 kb on + strand at 657.674 kb on + strand at 657.698 kb on + strand at 657.708 kb on - strand at 657.709 kb on + strand at 657.709 kb on + strand at 657.717 kb on - strand at 657.717 kb on - strand at 657.786 kb on + strand at 657.926 kb on - strand at 657.964 kb on - strand at 658.081 kb on + strand at 658.103 kb on - strand at 658.139 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in media lacking HCO3 buffered with 10 mM TES pH8 remove 645,156 + SGL_RS04900 0.69 -0.2 645,161 + SGL_RS04900 0.70 -0.4 645,173 - SGL_RS04900 0.71 +0.3 645,186 - SGL_RS04900 0.72 -0.0 645,232 + SGL_RS04900 0.76 -0.1 645,278 - SGL_RS04900 0.80 +0.2 645,315 + SGL_RS04900 0.84 -0.4 645,323 - SGL_RS04900 0.84 +0.1 645,367 - SGL_RS04900 0.88 +0.3 645,691 - +0.2 645,701 + -0.2 645,724 - SGL_RS19435 0.20 +0.5 645,966 - -0.0 645,968 + +0.4 646,007 + +0.2 646,165 + -0.9 646,173 - -0.4 646,173 - +0.1 646,173 - -0.3 646,228 + +0.0 646,236 - +0.0 646,401 - -0.2 646,479 - +2.3 646,611 - +0.5 646,672 - -0.1 646,761 + -0.1 646,761 + +0.2 646,780 + +0.1 646,840 - +0.0 646,869 - +0.2 647,223 + -0.0 647,254 - +0.5 647,334 - SGL_RS04910 0.10 -0.4 647,369 - SGL_RS04910 0.10 +0.2 647,392 + SGL_RS04910 0.11 -0.1 647,425 + SGL_RS04910 0.11 +0.5 647,434 + SGL_RS04910 0.11 +0.2 647,459 - SGL_RS04910 0.11 -0.0 647,822 + SGL_RS04910 0.14 -0.5 647,860 - SGL_RS04910 0.15 -0.4 647,895 - SGL_RS04910 0.15 +1.1 647,951 - SGL_RS04910 0.15 +0.6 647,975 + SGL_RS04910 0.15 -1.3 647,980 + SGL_RS04910 0.16 +0.2 647,985 + SGL_RS04910 0.16 +0.2 647,985 + SGL_RS04910 0.16 +1.1 647,988 - SGL_RS04910 0.16 -0.0 647,993 - SGL_RS04910 0.16 -0.3 648,145 + SGL_RS04910 0.17 -0.6 648,145 + SGL_RS04910 0.17 -0.6 648,209 - SGL_RS04910 0.17 +0.1 648,573 + SGL_RS04910 0.21 +0.2 649,336 + SGL_RS04910 0.27 +0.1 649,543 + SGL_RS04910 0.29 +0.3 649,546 + SGL_RS04910 0.29 +0.3 649,628 + SGL_RS04910 0.29 -0.4 649,695 - SGL_RS04910 0.30 +0.3 649,745 - SGL_RS04910 0.30 +0.1 649,780 - SGL_RS04910 0.31 -0.1 649,814 + SGL_RS04910 0.31 +0.1 650,010 + SGL_RS04910 0.33 +0.1 650,082 + SGL_RS04910 0.33 -0.5 650,115 + SGL_RS04910 0.33 +0.2 650,123 - SGL_RS04910 0.34 -0.0 650,123 - SGL_RS04910 0.34 +0.8 650,152 - SGL_RS04910 0.34 -0.1 650,293 + SGL_RS04910 0.35 +3.3 650,341 + SGL_RS04910 0.35 -0.1 650,426 - SGL_RS04910 0.36 -0.4 650,740 - SGL_RS04910 0.39 -0.5 650,960 - SGL_RS04910 0.41 -2.2 651,028 + SGL_RS04910 0.41 -0.7 651,036 - SGL_RS04910 0.41 +0.1 651,038 + SGL_RS04910 0.41 -0.0 651,046 - SGL_RS04910 0.41 -0.2 651,073 + SGL_RS04910 0.41 -0.2 651,435 - SGL_RS04910 0.45 -0.1 651,722 + SGL_RS04910 0.47 +0.4 651,749 - SGL_RS04910 0.47 -0.5 651,778 + SGL_RS04910 0.47 -0.5 651,916 + SGL_RS04910 0.49 -0.7 651,916 + SGL_RS04910 0.49 +0.4 652,007 + SGL_RS04910 0.49 -0.7 652,021 - SGL_RS04910 0.49 -0.7 652,067 + SGL_RS04910 0.50 -0.3 652,269 + SGL_RS04910 0.52 -0.4 652,350 - SGL_RS04910 0.52 +0.2 652,547 + SGL_RS04910 0.54 -0.3 652,558 + SGL_RS04910 0.54 +0.2 652,648 - SGL_RS04910 0.55 -0.1 652,702 + SGL_RS04910 0.55 -0.7 652,748 + SGL_RS04910 0.56 -0.1 652,774 + SGL_RS04910 0.56 +0.6 652,859 - SGL_RS04910 0.56 +0.5 652,885 + SGL_RS04910 0.57 +0.3 652,921 + SGL_RS04910 0.57 +0.0 653,325 + SGL_RS04910 0.60 -1.0 653,373 + SGL_RS04910 0.61 -0.1 653,463 + SGL_RS04910 0.62 -0.5 653,533 + SGL_RS04910 0.62 -1.0 653,596 + SGL_RS04910 0.63 +0.1 653,727 + SGL_RS04910 0.64 -0.4 653,808 + SGL_RS04910 0.64 -0.0 654,015 + SGL_RS04910 0.66 +0.1 654,021 + SGL_RS04910 0.66 -0.0 654,074 + SGL_RS04910 0.67 -0.6 654,136 - SGL_RS04910 0.67 +0.0 654,140 + SGL_RS04910 0.67 +0.7 654,146 - SGL_RS04910 0.67 -0.2 654,150 + SGL_RS04910 0.67 -0.1 654,158 - SGL_RS04910 0.67 -0.6 654,215 + SGL_RS04910 0.68 -0.3 654,222 - SGL_RS04910 0.68 +2.3 654,447 - SGL_RS04910 0.70 -0.4 654,467 + SGL_RS04910 0.70 +0.2 654,470 - SGL_RS04910 0.70 -0.1 654,682 - SGL_RS04910 0.72 -1.3 654,704 + SGL_RS04910 0.72 -0.2 654,707 + SGL_RS04910 0.72 +0.2 654,837 - SGL_RS04910 0.73 +0.6 654,968 + SGL_RS04910 0.74 -0.4 655,185 - SGL_RS04910 0.76 -0.1 655,189 + SGL_RS04910 0.76 +0.3 655,409 + SGL_RS04910 0.78 -0.6 655,586 - SGL_RS04910 0.79 -0.1 655,659 - SGL_RS04910 0.80 -0.9 655,735 + SGL_RS04910 0.81 -0.5 656,225 + SGL_RS04910 0.85 +0.0 656,238 - SGL_RS04910 0.85 -0.2 656,256 + SGL_RS04910 0.85 -0.3 656,267 + SGL_RS04910 0.85 +0.0 656,275 - SGL_RS04910 0.85 -0.4 656,299 - SGL_RS04910 0.85 +0.5 656,311 + SGL_RS04910 0.85 -0.0 656,402 + SGL_RS04910 0.86 -0.3 656,430 - SGL_RS04910 0.86 +0.0 656,523 + SGL_RS04910 0.87 -0.3 656,713 + SGL_RS04910 0.89 +0.0 656,769 - SGL_RS04910 0.89 -0.4 656,928 - +0.3 656,965 - -0.1 657,093 + +0.6 657,135 - -0.7 657,226 - -0.1 657,286 + +0.1 657,286 + -0.8 657,288 - -0.2 657,622 + -1.1 657,674 + -0.2 657,698 + +0.5 657,708 - +0.0 657,709 + -0.3 657,709 + +0.0 657,717 - +0.1 657,717 - -0.7 657,786 + -0.6 657,926 - +0.5 657,964 - -0.3 658,081 + -0.3 658,103 - -0.2 658,139 + +0.2
Or see this region's nucleotide sequence