Experiment: growth in media lacking HCO3 buffered with 10 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04385 and SGL_RS04390 are separated by 157 nucleotides SGL_RS04390 and SGL_RS04395 overlap by 4 nucleotides SGL_RS04395 and SGL_RS04400 are separated by 2 nucleotides SGL_RS04400 and SGL_RS04405 overlap by 4 nucleotides SGL_RS04405 and SGL_RS04410 are separated by 59 nucleotides
SGL_RS04385: SGL_RS04385 - glutamate decarboxylase, at 543,468 to 544,871
_RS04385
SGL_RS04390: SGL_RS04390 - DUF433 domain-containing protein, at 545,029 to 545,253
_RS04390
SGL_RS04395: SGL_RS04395 - DUF5615 family PIN-like protein, at 545,250 to 545,588
_RS04395
SGL_RS04400: SGL_RS04400 - carbon-nitrogen hydrolase family protein, at 545,591 to 546,556
_RS04400
SGL_RS04405: SGL_RS04405 - urease accessory protein UreD, at 546,553 to 547,365
_RS04405
SGL_RS04410: SGL_RS04410 - photosystem II protein PsbQ, at 547,425 to 547,874
_RS04410
Position (kb) 545
546
547 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 544.662 kb on + strand, within SGL_RS04385 at 544.794 kb on + strand at 544.842 kb on - strand at 545.078 kb on - strand, within SGL_RS04390 at 545.194 kb on + strand, within SGL_RS04390 at 545.211 kb on - strand, within SGL_RS04390 at 545.353 kb on + strand, within SGL_RS04395 at 545.388 kb on + strand, within SGL_RS04395 at 545.516 kb on + strand, within SGL_RS04395 at 545.546 kb on + strand, within SGL_RS04395 at 545.546 kb on + strand, within SGL_RS04395 at 545.613 kb on + strand at 545.621 kb on - strand at 545.672 kb on + strand at 545.672 kb on + strand at 545.792 kb on - strand, within SGL_RS04400 at 545.885 kb on - strand, within SGL_RS04400 at 545.896 kb on + strand, within SGL_RS04400 at 545.997 kb on - strand, within SGL_RS04400 at 546.334 kb on - strand, within SGL_RS04400 at 546.401 kb on + strand, within SGL_RS04400 at 546.467 kb on - strand at 546.665 kb on - strand, within SGL_RS04405 at 546.776 kb on + strand, within SGL_RS04405 at 546.988 kb on - strand, within SGL_RS04405 at 547.044 kb on + strand, within SGL_RS04405 at 547.044 kb on + strand, within SGL_RS04405 at 547.074 kb on + strand, within SGL_RS04405 at 547.135 kb on + strand, within SGL_RS04405 at 547.147 kb on + strand, within SGL_RS04405 at 547.187 kb on + strand, within SGL_RS04405 at 547.192 kb on + strand, within SGL_RS04405 at 547.195 kb on - strand, within SGL_RS04405 at 547.212 kb on + strand, within SGL_RS04405 at 547.290 kb on + strand at 547.290 kb on + strand at 547.290 kb on - strand at 547.520 kb on - strand, within SGL_RS04410
Per-strain Table
Position Strand Gene LocusTag Fraction growth in media lacking HCO3 buffered with 10 mM TES pH8 remove 544,662 + SGL_RS04385 0.85 +0.0 544,794 + -0.4 544,842 - +0.0 545,078 - SGL_RS04390 0.22 -0.8 545,194 + SGL_RS04390 0.73 -3.3 545,211 - SGL_RS04390 0.81 -0.5 545,353 + SGL_RS04395 0.30 -0.0 545,388 + SGL_RS04395 0.41 +0.1 545,516 + SGL_RS04395 0.78 -0.2 545,546 + SGL_RS04395 0.87 -1.1 545,546 + SGL_RS04395 0.87 -0.1 545,613 + +0.0 545,621 - +0.3 545,672 + -0.1 545,672 + +0.3 545,792 - SGL_RS04400 0.21 -0.1 545,885 - SGL_RS04400 0.30 -0.0 545,896 + SGL_RS04400 0.32 -0.1 545,997 - SGL_RS04400 0.42 -0.7 546,334 - SGL_RS04400 0.77 -0.1 546,401 + SGL_RS04400 0.84 -0.3 546,467 - -0.0 546,665 - SGL_RS04405 0.14 +0.1 546,776 + SGL_RS04405 0.27 -0.1 546,988 - SGL_RS04405 0.54 +0.1 547,044 + SGL_RS04405 0.60 +0.5 547,044 + SGL_RS04405 0.60 -0.1 547,074 + SGL_RS04405 0.64 -0.2 547,135 + SGL_RS04405 0.72 +0.3 547,147 + SGL_RS04405 0.73 -0.3 547,187 + SGL_RS04405 0.78 +0.1 547,192 + SGL_RS04405 0.79 +0.4 547,195 - SGL_RS04405 0.79 +0.3 547,212 + SGL_RS04405 0.81 -0.2 547,290 + -0.7 547,290 + +0.2 547,290 - +0.1 547,520 - SGL_RS04410 0.21 +0.3
Or see this region's nucleotide sequence