Experiment: Na NO3 10 mM as N source, buffered with 20 mM TES pH8 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14915 and SGL_RS14920 are separated by 125 nucleotides SGL_RS14920 and SGL_RS14925 are separated by 225 nucleotides SGL_RS14925 and SGL_RS14930 are separated by 34 nucleotides
SGL_RS14915: SGL_RS14915 - ABC transporter substrate-binding protein, at 2,799,650 to 2,801,014
_RS14915
SGL_RS14920: SGL_RS14920 - cation:proton antiporter, at 2,801,140 to 2,803,035
_RS14920
SGL_RS14925: SGL_RS14925 - tRNA-Cys, at 2,803,261 to 2,803,331
_RS14925
SGL_RS14930: SGL_RS14930 - type I methionyl aminopeptidase, at 2,803,366 to 2,804,208
_RS14930
Position (kb)
2801
2802
2803
2804 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2800.148 kb on - strand, within SGL_RS14915 at 2800.148 kb on - strand, within SGL_RS14915 at 2800.418 kb on + strand, within SGL_RS14915 at 2800.426 kb on - strand, within SGL_RS14915 at 2800.426 kb on - strand, within SGL_RS14915 at 2800.426 kb on - strand, within SGL_RS14915 at 2800.971 kb on + strand at 2800.971 kb on + strand at 2801.288 kb on + strand at 2802.110 kb on - strand, within SGL_RS14920 at 2802.135 kb on + strand, within SGL_RS14920 at 2802.188 kb on + strand, within SGL_RS14920 at 2802.527 kb on + strand, within SGL_RS14920 at 2802.582 kb on - strand, within SGL_RS14920 at 2802.780 kb on + strand, within SGL_RS14920 at 2802.904 kb on + strand at 2803.071 kb on + strand at 2803.141 kb on - strand at 2803.563 kb on + strand, within SGL_RS14930 at 2803.566 kb on - strand, within SGL_RS14930 at 2803.625 kb on - strand, within SGL_RS14930 at 2803.646 kb on + strand, within SGL_RS14930 at 2803.781 kb on + strand, within SGL_RS14930 at 2803.781 kb on + strand, within SGL_RS14930 at 2803.781 kb on + strand, within SGL_RS14930 at 2803.781 kb on + strand, within SGL_RS14930
Per-strain Table
Position Strand Gene LocusTag Fraction Na NO3 10 mM as N source, buffered with 20 mM TES pH8 but diluted to prevent light attenuation remove 2,800,148 - SGL_RS14915 0.36 +0.2 2,800,148 - SGL_RS14915 0.36 +1.5 2,800,418 + SGL_RS14915 0.56 +0.2 2,800,426 - SGL_RS14915 0.57 -0.4 2,800,426 - SGL_RS14915 0.57 +0.5 2,800,426 - SGL_RS14915 0.57 +0.1 2,800,971 + -0.6 2,800,971 + +0.0 2,801,288 + -2.2 2,802,110 - SGL_RS14920 0.51 -1.7 2,802,135 + SGL_RS14920 0.52 -0.4 2,802,188 + SGL_RS14920 0.55 -1.7 2,802,527 + SGL_RS14920 0.73 -2.0 2,802,582 - SGL_RS14920 0.76 -1.9 2,802,780 + SGL_RS14920 0.86 -0.4 2,802,904 + -0.9 2,803,071 + -1.1 2,803,141 - +0.1 2,803,563 + SGL_RS14930 0.23 -2.1 2,803,566 - SGL_RS14930 0.24 -2.0 2,803,625 - SGL_RS14930 0.31 -2.0 2,803,646 + SGL_RS14930 0.33 -1.9 2,803,781 + SGL_RS14930 0.49 -2.4 2,803,781 + SGL_RS14930 0.49 -2.4 2,803,781 + SGL_RS14930 0.49 -3.0 2,803,781 + SGL_RS14930 0.49 -1.9
Or see this region's nucleotide sequence