Experiment: Na NO3 10 mM as N source, buffered with 20 mM TES pH8 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS09880 and SGL_RS09885 are separated by 461 nucleotides SGL_RS09885 and SGL_RS09890 are separated by 56 nucleotides SGL_RS09890 and SGL_RS09895 are separated by 57 nucleotides SGL_RS09895 and SGL_RS19875 are separated by 11 nucleotides SGL_RS19875 and SGL_RS19215 are separated by 20 nucleotides
SGL_RS09880: SGL_RS09880 - hypothetical protein, at 1,699,970 to 1,700,431
_RS09880
SGL_RS09885: SGL_RS09885 - NAD(P)H-quinone oxidoreductase subunit L, at 1,700,893 to 1,701,135
_RS09885
SGL_RS09890: SGL_RS09890 - DUF3007 family protein, at 1,701,192 to 1,701,557
_RS09890
SGL_RS09895: SGL_RS09895 - DUF177 domain-containing protein, at 1,701,615 to 1,702,118
_RS09895
SGL_RS19875: SGL_RS19875 - IS1 family transposase, at 1,702,130 to 1,702,279
_RS19875
SGL_RS19215: SGL_RS19215 - transposase, at 1,702,300 to 1,703,319
_RS19215
Position (kb) 1701
1702 Strain fitness (log2 ratio) -2
-1
0
1 at 1700.206 kb on + strand, within SGL_RS09880 at 1700.241 kb on + strand, within SGL_RS09880 at 1700.267 kb on + strand, within SGL_RS09880 at 1700.285 kb on - strand, within SGL_RS09880 at 1700.389 kb on - strand at 1700.427 kb on + strand at 1700.603 kb on - strand at 1700.611 kb on + strand at 1701.160 kb on - strand at 1701.220 kb on - strand at 1701.331 kb on + strand, within SGL_RS09890 at 1701.371 kb on - strand, within SGL_RS09890 at 1701.376 kb on - strand, within SGL_RS09890 at 1701.452 kb on - strand, within SGL_RS09890 at 1701.481 kb on + strand, within SGL_RS09890 at 1701.582 kb on - strand at 1701.624 kb on - strand at 1701.717 kb on - strand, within SGL_RS09895 at 1701.717 kb on - strand, within SGL_RS09895 at 1701.820 kb on + strand, within SGL_RS09895 at 1701.916 kb on + strand, within SGL_RS09895 at 1701.991 kb on - strand, within SGL_RS09895 at 1702.128 kb on + strand at 1702.176 kb on + strand, within SGL_RS19875 at 1702.199 kb on - strand, within SGL_RS19875 at 1702.493 kb on - strand, within SGL_RS19215 at 1702.549 kb on - strand, within SGL_RS19215
Per-strain Table
Position Strand Gene LocusTag Fraction Na NO3 10 mM as N source, buffered with 20 mM TES pH8 but diluted to prevent light attenuation remove 1,700,206 + SGL_RS09880 0.51 +0.4 1,700,241 + SGL_RS09880 0.59 -0.1 1,700,267 + SGL_RS09880 0.64 -0.2 1,700,285 - SGL_RS09880 0.68 +0.0 1,700,389 - -0.4 1,700,427 + +0.1 1,700,603 - -0.3 1,700,611 + +0.5 1,701,160 - +0.2 1,701,220 - -0.1 1,701,331 + SGL_RS09890 0.38 -1.4 1,701,371 - SGL_RS09890 0.49 -1.8 1,701,376 - SGL_RS09890 0.50 -1.3 1,701,452 - SGL_RS09890 0.71 -1.9 1,701,481 + SGL_RS09890 0.79 -0.3 1,701,582 - -0.0 1,701,624 - +0.2 1,701,717 - SGL_RS09895 0.20 -0.0 1,701,717 - SGL_RS09895 0.20 -0.0 1,701,820 + SGL_RS09895 0.41 +0.6 1,701,916 + SGL_RS09895 0.60 -0.3 1,701,991 - SGL_RS09895 0.75 +0.5 1,702,128 + -0.3 1,702,176 + SGL_RS19875 0.31 -0.9 1,702,199 - SGL_RS19875 0.46 +1.5 1,702,493 - SGL_RS19215 0.19 +0.2 1,702,549 - SGL_RS19215 0.24 -0.2
Or see this region's nucleotide sequence