Experiment: Na NO3 10 mM as N source, buffered with 20 mM TES pH8 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS06015 and SGL_RS06020 are separated by 115 nucleotides SGL_RS06020 and SGL_RS06025 are separated by 109 nucleotides SGL_RS06025 and SGL_RS06030 are separated by 204 nucleotides
SGL_RS06015: SGL_RS06015 - urea ABC transporter permease subunit UrtC, at 883,287 to 884,519
_RS06015
SGL_RS06020: SGL_RS06020 - sugar ABC transporter permease, at 884,635 to 885,531
_RS06020
SGL_RS06025: SGL_RS06025 - DUF29 domain-containing protein, at 885,641 to 886,078
_RS06025
SGL_RS06030: SGL_RS06030 - HhoA/HhoB/HtrA family serine endopeptidase, at 886,283 to 887,542
_RS06030
Position (kb) 884
885
886 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 883.718 kb on + strand, within SGL_RS06015 at 883.752 kb on + strand, within SGL_RS06015 at 884.005 kb on - strand, within SGL_RS06015 at 884.053 kb on + strand, within SGL_RS06015 at 884.091 kb on - strand, within SGL_RS06015 at 884.129 kb on + strand, within SGL_RS06015 at 884.137 kb on - strand, within SGL_RS06015 at 884.138 kb on + strand, within SGL_RS06015 at 884.146 kb on - strand, within SGL_RS06015 at 884.146 kb on - strand, within SGL_RS06015 at 884.146 kb on - strand, within SGL_RS06015 at 884.146 kb on - strand, within SGL_RS06015 at 884.146 kb on - strand, within SGL_RS06015 at 884.146 kb on - strand, within SGL_RS06015 at 884.146 kb on - strand, within SGL_RS06015 at 884.146 kb on - strand, within SGL_RS06015 at 884.146 kb on - strand, within SGL_RS06015 at 884.146 kb on - strand, within SGL_RS06015 at 884.156 kb on - strand, within SGL_RS06015 at 884.167 kb on - strand, within SGL_RS06015 at 884.169 kb on - strand, within SGL_RS06015 at 884.209 kb on + strand, within SGL_RS06015 at 884.238 kb on - strand, within SGL_RS06015 at 884.240 kb on + strand, within SGL_RS06015 at 884.268 kb on - strand, within SGL_RS06015 at 884.280 kb on - strand, within SGL_RS06015 at 884.363 kb on - strand, within SGL_RS06015 at 884.398 kb on - strand at 884.500 kb on - strand at 884.504 kb on + strand at 884.512 kb on - strand at 884.512 kb on - strand at 884.671 kb on - strand at 884.672 kb on - strand at 884.971 kb on + strand, within SGL_RS06020 at 884.971 kb on + strand, within SGL_RS06020 at 884.979 kb on - strand, within SGL_RS06020 at 884.979 kb on - strand, within SGL_RS06020 at 884.979 kb on - strand, within SGL_RS06020 at 884.979 kb on - strand, within SGL_RS06020 at 884.979 kb on - strand, within SGL_RS06020 at 884.979 kb on - strand, within SGL_RS06020 at 884.979 kb on - strand, within SGL_RS06020 at 885.112 kb on - strand, within SGL_RS06020 at 885.159 kb on - strand, within SGL_RS06020 at 885.446 kb on - strand at 885.527 kb on + strand at 885.902 kb on - strand, within SGL_RS06025 at 886.264 kb on + strand at 886.280 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Na NO3 10 mM as N source, buffered with 20 mM TES pH8 but diluted to prevent light attenuation remove 883,718 + SGL_RS06015 0.35 -0.0 883,752 + SGL_RS06015 0.38 +0.1 884,005 - SGL_RS06015 0.58 +0.0 884,053 + SGL_RS06015 0.62 -0.0 884,091 - SGL_RS06015 0.65 +0.6 884,129 + SGL_RS06015 0.68 -0.1 884,137 - SGL_RS06015 0.69 +0.7 884,138 + SGL_RS06015 0.69 -0.6 884,146 - SGL_RS06015 0.70 +0.3 884,146 - SGL_RS06015 0.70 -3.0 884,146 - SGL_RS06015 0.70 +0.2 884,146 - SGL_RS06015 0.70 -0.2 884,146 - SGL_RS06015 0.70 -0.0 884,146 - SGL_RS06015 0.70 -0.1 884,146 - SGL_RS06015 0.70 -0.9 884,146 - SGL_RS06015 0.70 -0.4 884,146 - SGL_RS06015 0.70 -1.2 884,146 - SGL_RS06015 0.70 -0.4 884,156 - SGL_RS06015 0.70 -0.4 884,167 - SGL_RS06015 0.71 -1.4 884,169 - SGL_RS06015 0.72 -0.0 884,209 + SGL_RS06015 0.75 -0.8 884,238 - SGL_RS06015 0.77 -0.4 884,240 + SGL_RS06015 0.77 +0.8 884,268 - SGL_RS06015 0.80 -0.2 884,280 - SGL_RS06015 0.81 -0.6 884,363 - SGL_RS06015 0.87 -0.1 884,398 - +0.3 884,500 - +1.3 884,504 + +0.2 884,512 - -0.0 884,512 - -0.4 884,671 - -2.3 884,672 - +0.4 884,971 + SGL_RS06020 0.37 -0.2 884,971 + SGL_RS06020 0.37 -1.6 884,979 - SGL_RS06020 0.38 -2.0 884,979 - SGL_RS06020 0.38 -0.6 884,979 - SGL_RS06020 0.38 -2.4 884,979 - SGL_RS06020 0.38 -1.5 884,979 - SGL_RS06020 0.38 -2.5 884,979 - SGL_RS06020 0.38 -0.4 884,979 - SGL_RS06020 0.38 -2.4 885,112 - SGL_RS06020 0.53 +0.2 885,159 - SGL_RS06020 0.58 -0.1 885,446 - -2.0 885,527 + +0.2 885,902 - SGL_RS06025 0.60 -0.4 886,264 + -0.6 886,280 + -0.1
Or see this region's nucleotide sequence