Experiment: Na NO3 10 mM as N source, buffered with 20 mM TES pH8 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS02450 and SGL_RS02455 overlap by 20 nucleotides SGL_RS02455 and SGL_RS02460 are separated by 76 nucleotides SGL_RS02460 and SGL_RS02465 are separated by 161 nucleotides SGL_RS02465 and SGL_RS02470 are separated by 75 nucleotides
SGL_RS02450: SGL_RS02450 - hypothetical protein, at 129,791 to 130,186
_RS02450
SGL_RS02455: SGL_RS02455 - CPBP family intramembrane metalloprotease, at 130,167 to 131,039
_RS02455
SGL_RS02460: SGL_RS02460 - phosphate signaling complex protein PhoU, at 131,116 to 131,790
_RS02460
SGL_RS02465: SGL_RS02465 - ribosome maturation factor RimP, at 131,952 to 132,407
_RS02465
SGL_RS02470: SGL_RS02470 - transcription termination factor NusA, at 132,483 to 133,859
_RS02470
Position (kb) 131
132 Strain fitness (log2 ratio) -2
-1
0
1 at 130.130 kb on - strand, within SGL_RS02450 at 130.136 kb on - strand, within SGL_RS02450 at 131.057 kb on + strand at 131.067 kb on + strand at 131.165 kb on - strand at 131.173 kb on + strand at 131.181 kb on - strand at 131.196 kb on - strand, within SGL_RS02460 at 131.388 kb on + strand, within SGL_RS02460 at 131.639 kb on + strand, within SGL_RS02460 at 131.645 kb on - strand, within SGL_RS02460 at 131.646 kb on - strand, within SGL_RS02460 at 131.785 kb on + strand at 131.844 kb on + strand at 132.380 kb on + strand at 132.382 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Na NO3 10 mM as N source, buffered with 20 mM TES pH8 but diluted to prevent light attenuation remove 130,130 - SGL_RS02450 0.86 -0.0 130,136 - SGL_RS02450 0.87 -0.1 131,057 + +0.3 131,067 + -0.0 131,165 - +0.4 131,173 + -0.3 131,181 - -0.9 131,196 - SGL_RS02460 0.12 -1.9 131,388 + SGL_RS02460 0.40 -0.1 131,639 + SGL_RS02460 0.77 -0.4 131,645 - SGL_RS02460 0.78 -1.1 131,646 - SGL_RS02460 0.79 -0.9 131,785 + +0.3 131,844 + -0.6 132,380 + -1.1 132,382 + -1.6
Or see this region's nucleotide sequence