Experiment: ClNH4 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS15740 and SGL_RS15745 are separated by 98 nucleotides SGL_RS15745 and SGL_RS15750 are separated by 128 nucleotides
SGL_RS15740: SGL_RS15740 - M20 family metallopeptidase, at 2,981,009 to 2,982,190
_RS15740
SGL_RS15745: SGL_RS15745 - bifunctional cobalt-precorrin-7 (C(5))-methyltransferase/cobalt-precorrin-6B (C(15))-methyltransferase, at 2,982,289 to 2,983,566
_RS15745
SGL_RS15750: SGL_RS15750 - 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN, at 2,983,695 to 2,984,747
_RS15750
Position (kb)
2982
2983
2984 Strain fitness (log2 ratio)
-1
0
1 at 2981.313 kb on - strand, within SGL_RS15740 at 2981.326 kb on - strand, within SGL_RS15740 at 2981.379 kb on - strand, within SGL_RS15740 at 2981.392 kb on + strand, within SGL_RS15740 at 2981.424 kb on + strand, within SGL_RS15740 at 2981.424 kb on + strand, within SGL_RS15740 at 2981.552 kb on - strand, within SGL_RS15740 at 2981.555 kb on - strand, within SGL_RS15740 at 2981.593 kb on + strand, within SGL_RS15740 at 2981.593 kb on + strand, within SGL_RS15740 at 2981.606 kb on + strand, within SGL_RS15740 at 2981.661 kb on + strand, within SGL_RS15740 at 2981.724 kb on - strand, within SGL_RS15740 at 2981.752 kb on - strand, within SGL_RS15740 at 2981.827 kb on - strand, within SGL_RS15740 at 2981.827 kb on - strand, within SGL_RS15740 at 2981.847 kb on + strand, within SGL_RS15740 at 2981.848 kb on - strand, within SGL_RS15740 at 2981.848 kb on - strand, within SGL_RS15740 at 2981.850 kb on - strand, within SGL_RS15740 at 2981.855 kb on - strand, within SGL_RS15740 at 2981.867 kb on + strand, within SGL_RS15740 at 2981.888 kb on + strand, within SGL_RS15740 at 2981.889 kb on - strand, within SGL_RS15740 at 2982.035 kb on + strand, within SGL_RS15740 at 2982.060 kb on - strand, within SGL_RS15740 at 2982.062 kb on + strand, within SGL_RS15740 at 2982.221 kb on + strand at 2982.229 kb on - strand at 2982.229 kb on - strand at 2982.229 kb on - strand at 2982.272 kb on - strand at 2982.291 kb on + strand at 2982.721 kb on - strand, within SGL_RS15745 at 2982.790 kb on + strand, within SGL_RS15745 at 2983.260 kb on - strand, within SGL_RS15745 at 2983.537 kb on + strand at 2983.537 kb on + strand at 2983.764 kb on + strand at 2983.764 kb on + strand at 2983.772 kb on - strand at 2983.772 kb on - strand at 2983.798 kb on - strand at 2983.821 kb on - strand, within SGL_RS15750 at 2983.931 kb on - strand, within SGL_RS15750 at 2983.959 kb on - strand, within SGL_RS15750 at 2983.984 kb on - strand, within SGL_RS15750 at 2983.990 kb on - strand, within SGL_RS15750 at 2984.062 kb on + strand, within SGL_RS15750 at 2984.119 kb on + strand, within SGL_RS15750 at 2984.144 kb on - strand, within SGL_RS15750 at 2984.198 kb on + strand, within SGL_RS15750 at 2984.224 kb on - strand, within SGL_RS15750 at 2984.382 kb on - strand, within SGL_RS15750 at 2984.410 kb on + strand, within SGL_RS15750 at 2984.437 kb on + strand, within SGL_RS15750 at 2984.437 kb on + strand, within SGL_RS15750 at 2984.445 kb on - strand, within SGL_RS15750
Per-strain Table
Position Strand Gene LocusTag Fraction ClNH4 10 mM as N source, buffered with 20 mM TES pH8 remove 2,981,313 - SGL_RS15740 0.26 -0.4 2,981,326 - SGL_RS15740 0.27 -0.1 2,981,379 - SGL_RS15740 0.31 -0.3 2,981,392 + SGL_RS15740 0.32 -0.0 2,981,424 + SGL_RS15740 0.35 -0.1 2,981,424 + SGL_RS15740 0.35 +0.7 2,981,552 - SGL_RS15740 0.46 -0.5 2,981,555 - SGL_RS15740 0.46 +0.0 2,981,593 + SGL_RS15740 0.49 +0.3 2,981,593 + SGL_RS15740 0.49 +1.0 2,981,606 + SGL_RS15740 0.51 -0.0 2,981,661 + SGL_RS15740 0.55 +0.3 2,981,724 - SGL_RS15740 0.60 -0.9 2,981,752 - SGL_RS15740 0.63 +0.2 2,981,827 - SGL_RS15740 0.69 -1.1 2,981,827 - SGL_RS15740 0.69 +0.1 2,981,847 + SGL_RS15740 0.71 -0.1 2,981,848 - SGL_RS15740 0.71 +0.2 2,981,848 - SGL_RS15740 0.71 -0.3 2,981,850 - SGL_RS15740 0.71 +0.3 2,981,855 - SGL_RS15740 0.72 -0.3 2,981,867 + SGL_RS15740 0.73 -0.1 2,981,888 + SGL_RS15740 0.74 +0.0 2,981,889 - SGL_RS15740 0.74 +0.3 2,982,035 + SGL_RS15740 0.87 +0.0 2,982,060 - SGL_RS15740 0.89 -0.4 2,982,062 + SGL_RS15740 0.89 -0.0 2,982,221 + -0.2 2,982,229 - -0.1 2,982,229 - +0.1 2,982,229 - +0.3 2,982,272 - -0.2 2,982,291 + -0.1 2,982,721 - SGL_RS15745 0.34 +0.9 2,982,790 + SGL_RS15745 0.39 -0.1 2,983,260 - SGL_RS15745 0.76 -1.7 2,983,537 + -1.1 2,983,537 + +0.9 2,983,764 + -0.3 2,983,764 + +0.2 2,983,772 - +1.1 2,983,772 - -0.9 2,983,798 - +0.3 2,983,821 - SGL_RS15750 0.12 -0.1 2,983,931 - SGL_RS15750 0.22 +0.1 2,983,959 - SGL_RS15750 0.25 +0.0 2,983,984 - SGL_RS15750 0.27 -0.7 2,983,990 - SGL_RS15750 0.28 +0.1 2,984,062 + SGL_RS15750 0.35 +0.6 2,984,119 + SGL_RS15750 0.40 +0.4 2,984,144 - SGL_RS15750 0.43 +0.1 2,984,198 + SGL_RS15750 0.48 +0.0 2,984,224 - SGL_RS15750 0.50 +0.2 2,984,382 - SGL_RS15750 0.65 -0.6 2,984,410 + SGL_RS15750 0.68 -0.4 2,984,437 + SGL_RS15750 0.70 -0.3 2,984,437 + SGL_RS15750 0.70 -0.1 2,984,445 - SGL_RS15750 0.71 +0.3
Or see this region's nucleotide sequence