Experiment: ClNH4 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14270 and SGL_RS14275 are separated by 70 nucleotides SGL_RS14275 and SGL_RS14280 are separated by 255 nucleotides SGL_RS14280 and SGL_RS19475 are separated by 225 nucleotides SGL_RS19475 and SGL_RS14285 are separated by 54 nucleotides
SGL_RS14270: SGL_RS14270 - folate-binding protein YgfZ, at 2,656,139 to 2,657,077
_RS14270
SGL_RS14275: SGL_RS14275 - hypothetical protein, at 2,657,148 to 2,657,594
_RS14275
SGL_RS14280: SGL_RS14280 - adenosylcobinamide-GDP ribazoletransferase, at 2,657,850 to 2,658,632
_RS14280
SGL_RS19475: SGL_RS19475 - hypothetical protein, at 2,658,858 to 2,659,025
_RS19475
SGL_RS14285: SGL_RS14285 - hypothetical protein, at 2,659,080 to 2,659,865
_RS14285
Position (kb)
2657
2658
2659 Strain fitness (log2 ratio)
-2
-1
0
1 at 2656.863 kb on + strand, within SGL_RS14270 at 2656.905 kb on + strand, within SGL_RS14270 at 2656.913 kb on - strand, within SGL_RS14270 at 2656.922 kb on - strand, within SGL_RS14270 at 2656.938 kb on + strand, within SGL_RS14270 at 2656.990 kb on + strand at 2656.998 kb on - strand at 2657.000 kb on - strand at 2657.137 kb on + strand at 2657.193 kb on + strand, within SGL_RS14275 at 2657.196 kb on - strand, within SGL_RS14275 at 2657.224 kb on + strand, within SGL_RS14275 at 2657.246 kb on + strand, within SGL_RS14275 at 2657.307 kb on + strand, within SGL_RS14275 at 2657.396 kb on + strand, within SGL_RS14275 at 2657.411 kb on - strand, within SGL_RS14275 at 2657.413 kb on + strand, within SGL_RS14275 at 2657.457 kb on - strand, within SGL_RS14275 at 2657.489 kb on + strand, within SGL_RS14275 at 2657.605 kb on - strand at 2657.893 kb on + strand at 2658.020 kb on + strand, within SGL_RS14280 at 2658.118 kb on + strand, within SGL_RS14280 at 2658.197 kb on + strand, within SGL_RS14280 at 2658.324 kb on + strand, within SGL_RS14280 at 2658.332 kb on - strand, within SGL_RS14280 at 2658.510 kb on + strand, within SGL_RS14280 at 2658.696 kb on - strand at 2658.815 kb on - strand at 2658.816 kb on - strand at 2658.982 kb on + strand, within SGL_RS19475 at 2658.990 kb on - strand, within SGL_RS19475 at 2659.347 kb on + strand, within SGL_RS14285 at 2659.498 kb on - strand, within SGL_RS14285 at 2659.539 kb on + strand, within SGL_RS14285
Per-strain Table
Position Strand Gene LocusTag Fraction ClNH4 10 mM as N source, buffered with 20 mM TES pH8 remove 2,656,863 + SGL_RS14270 0.77 +0.5 2,656,905 + SGL_RS14270 0.82 +0.1 2,656,913 - SGL_RS14270 0.82 +0.8 2,656,922 - SGL_RS14270 0.83 +0.1 2,656,938 + SGL_RS14270 0.85 -0.4 2,656,990 + -0.1 2,656,998 - -0.2 2,657,000 - +0.2 2,657,137 + +0.9 2,657,193 + SGL_RS14275 0.10 +0.0 2,657,196 - SGL_RS14275 0.11 -0.2 2,657,224 + SGL_RS14275 0.17 +0.2 2,657,246 + SGL_RS14275 0.22 -0.9 2,657,307 + SGL_RS14275 0.36 +0.3 2,657,396 + SGL_RS14275 0.55 +0.5 2,657,411 - SGL_RS14275 0.59 -0.2 2,657,413 + SGL_RS14275 0.59 -0.0 2,657,457 - SGL_RS14275 0.69 -0.3 2,657,489 + SGL_RS14275 0.76 +0.2 2,657,605 - +0.5 2,657,893 + -1.1 2,658,020 + SGL_RS14280 0.22 -1.1 2,658,118 + SGL_RS14280 0.34 -0.1 2,658,197 + SGL_RS14280 0.44 -0.4 2,658,324 + SGL_RS14280 0.61 +0.2 2,658,332 - SGL_RS14280 0.62 -1.6 2,658,510 + SGL_RS14280 0.84 -1.4 2,658,696 - -0.0 2,658,815 - -0.1 2,658,816 - -0.1 2,658,982 + SGL_RS19475 0.74 -0.3 2,658,990 - SGL_RS19475 0.79 -1.4 2,659,347 + SGL_RS14285 0.34 -2.4 2,659,498 - SGL_RS14285 0.53 -1.1 2,659,539 + SGL_RS14285 0.58 +0.1
Or see this region's nucleotide sequence