Experiment: ClNH4 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS09940 and SGL_RS09945 are separated by 420 nucleotides SGL_RS09945 and SGL_RS09950 are separated by 154 nucleotides SGL_RS09950 and SGL_RS09955 are separated by 152 nucleotides
SGL_RS09940: SGL_RS09940 - IS630 transposase-related protein, at 1,710,814 to 1,711,659
_RS09940
SGL_RS09945: SGL_RS09945 - MoaD/ThiS family protein, at 1,712,080 to 1,712,409
_RS09945
SGL_RS09950: SGL_RS09950 - ribulose-phosphate 3-epimerase, at 1,712,564 to 1,713,256
_RS09950
SGL_RS09955: SGL_RS09955 - cation-transporting P-type ATPase, at 1,713,409 to 1,716,270
_RS09955
Position (kb)
1712
1713
1714 Strain fitness (log2 ratio)
-1
0
1 at 1711.572 kb on + strand, within SGL_RS09940 at 1711.793 kb on + strand at 1711.799 kb on + strand at 1711.873 kb on + strand at 1711.892 kb on - strand at 1711.978 kb on + strand at 1713.411 kb on + strand at 1713.419 kb on - strand at 1713.494 kb on + strand at 1713.502 kb on - strand at 1713.502 kb on - strand at 1713.511 kb on + strand at 1713.512 kb on - strand at 1713.556 kb on - strand at 1713.688 kb on - strand at 1713.688 kb on - strand at 1713.799 kb on + strand, within SGL_RS09955 at 1713.918 kb on - strand, within SGL_RS09955 at 1714.053 kb on - strand, within SGL_RS09955 at 1714.126 kb on + strand, within SGL_RS09955 at 1714.134 kb on - strand, within SGL_RS09955 at 1714.167 kb on - strand, within SGL_RS09955 at 1714.182 kb on + strand, within SGL_RS09955 at 1714.182 kb on + strand, within SGL_RS09955
Per-strain Table
Position Strand Gene LocusTag Fraction ClNH4 10 mM as N source, buffered with 20 mM TES pH8 remove 1,711,572 + SGL_RS09940 0.90 +0.0 1,711,793 + -0.2 1,711,799 + -0.2 1,711,873 + +0.1 1,711,892 - -0.2 1,711,978 + -0.2 1,713,411 + -0.0 1,713,419 - -0.2 1,713,494 + +0.1 1,713,502 - -0.8 1,713,502 - -0.0 1,713,511 + -0.1 1,713,512 - -0.1 1,713,556 - +0.1 1,713,688 - -0.6 1,713,688 - -0.2 1,713,799 + SGL_RS09955 0.14 +0.1 1,713,918 - SGL_RS09955 0.18 +0.1 1,714,053 - SGL_RS09955 0.23 -0.4 1,714,126 + SGL_RS09955 0.25 +0.2 1,714,134 - SGL_RS09955 0.25 +0.2 1,714,167 - SGL_RS09955 0.26 -0.1 1,714,182 + SGL_RS09955 0.27 +0.4 1,714,182 + SGL_RS09955 0.27 -1.2
Or see this region's nucleotide sequence