Experiment: ClNH4 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS08965 and SGL_RS08970 are separated by 509 nucleotides SGL_RS08970 and SGL_RS08975 are separated by 55 nucleotides
SGL_RS08965: SGL_RS08965 - chlorophyll a/b binding light-harvesting protein, at 1,517,576 to 1,518,604
_RS08965
SGL_RS08970: SGL_RS08970 - ribosome biogenesis GTPase YlqF, at 1,519,114 to 1,519,953
_RS08970
SGL_RS08975: SGL_RS08975 - redox-regulated ATPase YchF, at 1,520,009 to 1,521,100
_RS08975
Position (kb)
1519
1520 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1518.237 kb on + strand, within SGL_RS08965 at 1518.301 kb on - strand, within SGL_RS08965 at 1518.324 kb on - strand, within SGL_RS08965 at 1518.342 kb on + strand, within SGL_RS08965 at 1518.380 kb on - strand, within SGL_RS08965 at 1518.384 kb on - strand, within SGL_RS08965 at 1518.426 kb on + strand, within SGL_RS08965 at 1518.426 kb on + strand, within SGL_RS08965 at 1518.434 kb on - strand, within SGL_RS08965 at 1518.434 kb on - strand, within SGL_RS08965 at 1518.434 kb on - strand, within SGL_RS08965 at 1518.466 kb on + strand, within SGL_RS08965 at 1518.474 kb on - strand, within SGL_RS08965 at 1518.528 kb on + strand at 1518.747 kb on - strand at 1518.889 kb on + strand at 1518.900 kb on + strand at 1518.908 kb on - strand at 1518.913 kb on - strand at 1519.049 kb on + strand at 1519.064 kb on - strand at 1519.087 kb on - strand at 1519.118 kb on - strand at 1519.118 kb on - strand at 1519.370 kb on - strand, within SGL_RS08970 at 1519.549 kb on + strand, within SGL_RS08970 at 1519.551 kb on + strand, within SGL_RS08970 at 1519.907 kb on + strand at 1519.997 kb on + strand at 1520.014 kb on - strand at 1520.032 kb on + strand at 1520.037 kb on + strand at 1520.075 kb on + strand at 1520.176 kb on + strand, within SGL_RS08975 at 1520.328 kb on - strand, within SGL_RS08975 at 1520.424 kb on + strand, within SGL_RS08975 at 1520.503 kb on + strand, within SGL_RS08975 at 1520.582 kb on - strand, within SGL_RS08975 at 1520.582 kb on - strand, within SGL_RS08975 at 1520.776 kb on + strand, within SGL_RS08975 at 1520.840 kb on + strand, within SGL_RS08975 at 1520.913 kb on + strand, within SGL_RS08975
Per-strain Table
Position Strand Gene LocusTag Fraction ClNH4 10 mM as N source, buffered with 20 mM TES pH8 remove 1,518,237 + SGL_RS08965 0.64 -2.2 1,518,301 - SGL_RS08965 0.70 -1.9 1,518,324 - SGL_RS08965 0.73 -1.4 1,518,342 + SGL_RS08965 0.74 -2.6 1,518,380 - SGL_RS08965 0.78 -2.3 1,518,384 - SGL_RS08965 0.79 -1.0 1,518,426 + SGL_RS08965 0.83 -2.5 1,518,426 + SGL_RS08965 0.83 -1.5 1,518,434 - SGL_RS08965 0.83 -1.3 1,518,434 - SGL_RS08965 0.83 -1.5 1,518,434 - SGL_RS08965 0.83 -1.8 1,518,466 + SGL_RS08965 0.86 -1.9 1,518,474 - SGL_RS08965 0.87 -0.9 1,518,528 + -3.0 1,518,747 - -2.2 1,518,889 + -0.3 1,518,900 + -0.3 1,518,908 - -0.2 1,518,913 - -0.2 1,519,049 + -0.2 1,519,064 - +0.1 1,519,087 - -0.3 1,519,118 - +0.9 1,519,118 - +0.0 1,519,370 - SGL_RS08970 0.30 -1.0 1,519,549 + SGL_RS08970 0.52 -1.0 1,519,551 + SGL_RS08970 0.52 -1.4 1,519,907 + -1.4 1,519,997 + +0.1 1,520,014 - -0.7 1,520,032 + -0.1 1,520,037 + -0.4 1,520,075 + -0.2 1,520,176 + SGL_RS08975 0.15 -0.0 1,520,328 - SGL_RS08975 0.29 +0.2 1,520,424 + SGL_RS08975 0.38 -0.1 1,520,503 + SGL_RS08975 0.45 -0.3 1,520,582 - SGL_RS08975 0.52 -0.2 1,520,582 - SGL_RS08975 0.52 -0.7 1,520,776 + SGL_RS08975 0.70 +0.3 1,520,840 + SGL_RS08975 0.76 -0.1 1,520,913 + SGL_RS08975 0.83 -0.4
Or see this region's nucleotide sequence