Experiment: ClNH4 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS08505 and SGL_RS08510 are separated by 34 nucleotides SGL_RS08510 and SGL_RS08515 are separated by 4 nucleotides SGL_RS08515 and SGL_RS08520 are separated by 192 nucleotides SGL_RS08520 and SGL_RS08525 are separated by 371 nucleotides
SGL_RS08505: SGL_RS08505 - hypothetical protein, at 1,409,730 to 1,410,152
_RS08505
SGL_RS08510: SGL_RS08510 - histidinol-phosphatase, at 1,410,187 to 1,410,990
_RS08510
SGL_RS08515: SGL_RS08515 - histidinol-phosphate transaminase, at 1,410,995 to 1,412,044
_RS08515
SGL_RS08520: SGL_RS08520 - metalloregulator ArsR/SmtB family transcription factor, at 1,412,237 to 1,412,551
_RS08520
SGL_RS08525: SGL_RS08525 - aquaporin Z, at 1,412,923 to 1,413,666
_RS08525
Position (kb)
1410
1411
1412
1413 Strain fitness (log2 ratio)
-1
0
1 at 1410.031 kb on + strand, within SGL_RS08505 at 1412.453 kb on + strand, within SGL_RS08520 at 1412.455 kb on + strand, within SGL_RS08520 at 1412.473 kb on + strand, within SGL_RS08520 at 1412.528 kb on - strand at 1412.621 kb on + strand at 1412.629 kb on - strand at 1412.630 kb on + strand at 1412.683 kb on - strand at 1412.684 kb on - strand at 1412.727 kb on - strand at 1412.727 kb on - strand at 1412.731 kb on + strand at 1412.732 kb on + strand at 1412.863 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ClNH4 10 mM as N source, buffered with 20 mM TES pH8 remove 1,410,031 + SGL_RS08505 0.71 +0.0 1,412,453 + SGL_RS08520 0.69 -0.0 1,412,455 + SGL_RS08520 0.69 -0.4 1,412,473 + SGL_RS08520 0.75 -0.6 1,412,528 - +0.1 1,412,621 + +0.4 1,412,629 - -0.2 1,412,630 + -0.2 1,412,683 - +0.0 1,412,684 - -0.4 1,412,727 - -0.1 1,412,727 - +0.1 1,412,731 + +0.1 1,412,732 + -0.2 1,412,863 + +1.0
Or see this region's nucleotide sequence