Experiment: ClNH4 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04525 and SGL_RS04530 are separated by 85 nucleotides SGL_RS04530 and SGL_RS04535 are separated by 36 nucleotides SGL_RS04535 and SGL_RS04540 are separated by 9 nucleotides SGL_RS04540 and SGL_RS04545 are separated by 32 nucleotides SGL_RS04545 and SGL_RS04550 are separated by 84 nucleotides
SGL_RS04525: SGL_RS04525 - photosynthesis system II assembly factor Ycf48, at 569,544 to 570,572
_RS04525
SGL_RS04530: SGL_RS04530 - cytochrome b559 subunit alpha, at 570,658 to 570,903
_RS04530
SGL_RS04535: SGL_RS04535 - cytochrome b559 subunit beta, at 570,940 to 571,074
_RS04535
SGL_RS04540: SGL_RS04540 - photosystem II reaction center protein L, at 571,084 to 571,203
_RS04540
SGL_RS04545: SGL_RS04545 - photosystem II reaction center protein J, at 571,236 to 571,355
_RS04545
SGL_RS04550: SGL_RS04550 - glutamate 5-kinase, at 571,440 to 572,567
_RS04550
Position (kb) 571
572 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 570.277 kb on - strand, within SGL_RS04525 at 570.409 kb on + strand, within SGL_RS04525 at 571.281 kb on - strand, within SGL_RS04545 at 571.520 kb on - strand at 571.570 kb on + strand, within SGL_RS04550 at 571.808 kb on - strand, within SGL_RS04550 at 571.829 kb on - strand, within SGL_RS04550 at 571.829 kb on - strand, within SGL_RS04550 at 571.924 kb on + strand, within SGL_RS04550 at 571.998 kb on + strand, within SGL_RS04550 at 572.338 kb on - strand, within SGL_RS04550
Per-strain Table
Position Strand Gene LocusTag Fraction ClNH4 10 mM as N source, buffered with 20 mM TES pH8 remove 570,277 - SGL_RS04525 0.71 -0.6 570,409 + SGL_RS04525 0.84 -2.5 571,281 - SGL_RS04545 0.38 -1.2 571,520 - -1.4 571,570 + SGL_RS04550 0.12 -1.4 571,808 - SGL_RS04550 0.33 -3.0 571,829 - SGL_RS04550 0.34 -1.1 571,829 - SGL_RS04550 0.34 -2.2 571,924 + SGL_RS04550 0.43 -1.2 571,998 + SGL_RS04550 0.49 -1.3 572,338 - SGL_RS04550 0.80 -4.4
Or see this region's nucleotide sequence