Experiment: ClNH4 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS02470 and SGL_RS02475 are separated by 63 nucleotides SGL_RS02475 and SGL_RS02480 are separated by 259 nucleotides SGL_RS02480 and SGL_RS02485 are separated by 1 nucleotides SGL_RS02485 and SGL_RS20105 are separated by 63 nucleotides SGL_RS20105 and SGL_RS02490 overlap by 7 nucleotides
SGL_RS02470: SGL_RS02470 - transcription termination factor NusA, at 132,483 to 133,859
_RS02470
SGL_RS02475: SGL_RS02475 - YlxR family protein, at 133,923 to 134,177
_RS02475
SGL_RS02480: SGL_RS02480 - translation initiation factor IF-2, at 134,437 to 137,442
_RS02480
SGL_RS02485: SGL_RS02485 - glutathione S-transferase family protein, at 137,444 to 138,112
_RS02485
SGL_RS20105: SGL_RS20105 - hypothetical protein, at 138,176 to 138,301
_RS20105
SGL_RS02490: SGL_RS02490 - glycogen debranching protein GlgX, at 138,295 to 140,535
_RS02490
Position (kb) 134
135
136
137
138 Strain fitness (log2 ratio) -2
-1
0
1 at 133.662 kb on + strand, within SGL_RS02470 at 133.667 kb on + strand, within SGL_RS02470 at 133.667 kb on + strand, within SGL_RS02470 at 133.667 kb on + strand, within SGL_RS02470 at 133.675 kb on - strand, within SGL_RS02470 at 133.700 kb on - strand, within SGL_RS02470 at 133.768 kb on + strand at 133.832 kb on + strand at 133.866 kb on - strand at 133.893 kb on + strand at 134.002 kb on + strand, within SGL_RS02475 at 134.111 kb on + strand, within SGL_RS02475 at 137.476 kb on - strand at 137.503 kb on - strand at 137.801 kb on + strand, within SGL_RS02485 at 138.142 kb on + strand at 138.218 kb on + strand, within SGL_RS20105 at 138.370 kb on - strand at 138.378 kb on - strand at 138.429 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ClNH4 10 mM as N source, buffered with 20 mM TES pH8 remove 133,662 + SGL_RS02470 0.86 -2.1 133,667 + SGL_RS02470 0.86 -0.0 133,667 + SGL_RS02470 0.86 -0.0 133,667 + SGL_RS02470 0.86 -0.6 133,675 - SGL_RS02470 0.87 -0.7 133,700 - SGL_RS02470 0.88 -0.6 133,768 + -0.0 133,832 + +0.0 133,866 - -0.5 133,893 + -0.4 134,002 + SGL_RS02475 0.31 -1.0 134,111 + SGL_RS02475 0.74 +0.1 137,476 - +0.3 137,503 - -1.1 137,801 + SGL_RS02485 0.53 +0.3 138,142 + +1.3 138,218 + SGL_RS20105 0.33 -1.5 138,370 - -0.4 138,378 - +1.3 138,429 + -0.1
Or see this region's nucleotide sequence