Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS25325 and CA265_RS25330 overlap by 10 nucleotides CA265_RS25330 and CA265_RS25335 are separated by 212 nucleotides CA265_RS25335 and CA265_RS25340 are separated by 142 nucleotides CA265_RS25340 and CA265_RS25345 are separated by 85 nucleotides CA265_RS25345 and CA265_RS25350 are separated by 20 nucleotides
CA265_RS25325: CA265_RS25325 - ATPase, at 6,157,350 to 6,158,525
_RS25325
CA265_RS25330: CA265_RS25330 - DoxX family protein, at 6,158,516 to 6,158,974
_RS25330
CA265_RS25335: CA265_RS25335 - hypothetical protein, at 6,159,187 to 6,159,297
_RS25335
CA265_RS25340: CA265_RS25340 - 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD, at 6,159,440 to 6,160,852
_RS25340
CA265_RS25345: CA265_RS25345 - hypothetical protein, at 6,160,938 to 6,161,351
_RS25345
CA265_RS25350: CA265_RS25350 - serine hydrolase, at 6,161,372 to 6,162,688
_RS25350
Position (kb)
6159
6160
6161 Strain fitness (log2 ratio)
-2
-1
0
1 at 6158.444 kb on + strand at 6158.456 kb on + strand at 6158.510 kb on + strand at 6158.511 kb on - strand at 6158.512 kb on + strand at 6158.579 kb on + strand, within CA265_RS25330 at 6158.775 kb on - strand, within CA265_RS25330 at 6158.816 kb on + strand, within CA265_RS25330 at 6158.901 kb on - strand, within CA265_RS25330 at 6159.007 kb on + strand at 6159.055 kb on + strand at 6159.170 kb on + strand at 6159.170 kb on + strand at 6159.170 kb on + strand at 6159.170 kb on + strand at 6159.171 kb on - strand at 6159.200 kb on + strand, within CA265_RS25335 at 6159.445 kb on + strand at 6159.460 kb on + strand at 6159.470 kb on - strand at 6159.483 kb on + strand at 6159.484 kb on - strand at 6159.577 kb on - strand at 6159.697 kb on + strand, within CA265_RS25340 at 6159.716 kb on + strand, within CA265_RS25340 at 6159.717 kb on - strand, within CA265_RS25340 at 6159.737 kb on - strand, within CA265_RS25340 at 6159.780 kb on + strand, within CA265_RS25340 at 6159.780 kb on + strand, within CA265_RS25340 at 6159.802 kb on + strand, within CA265_RS25340 at 6159.807 kb on + strand, within CA265_RS25340 at 6159.839 kb on - strand, within CA265_RS25340 at 6159.888 kb on + strand, within CA265_RS25340 at 6159.962 kb on - strand, within CA265_RS25340 at 6159.975 kb on - strand, within CA265_RS25340 at 6159.975 kb on - strand, within CA265_RS25340 at 6159.994 kb on + strand, within CA265_RS25340 at 6160.019 kb on - strand, within CA265_RS25340 at 6160.022 kb on + strand, within CA265_RS25340 at 6160.023 kb on - strand, within CA265_RS25340 at 6160.030 kb on - strand, within CA265_RS25340 at 6160.036 kb on + strand, within CA265_RS25340 at 6160.078 kb on - strand, within CA265_RS25340 at 6160.078 kb on - strand, within CA265_RS25340 at 6160.097 kb on - strand, within CA265_RS25340 at 6160.217 kb on - strand, within CA265_RS25340 at 6160.217 kb on - strand, within CA265_RS25340 at 6160.368 kb on + strand, within CA265_RS25340 at 6160.373 kb on - strand, within CA265_RS25340 at 6160.419 kb on - strand, within CA265_RS25340 at 6160.427 kb on + strand, within CA265_RS25340 at 6160.429 kb on + strand, within CA265_RS25340 at 6160.429 kb on + strand, within CA265_RS25340 at 6160.493 kb on + strand, within CA265_RS25340 at 6160.497 kb on + strand, within CA265_RS25340 at 6160.498 kb on - strand, within CA265_RS25340 at 6160.508 kb on - strand, within CA265_RS25340 at 6160.536 kb on + strand, within CA265_RS25340 at 6160.549 kb on + strand, within CA265_RS25340 at 6160.553 kb on - strand, within CA265_RS25340 at 6160.563 kb on - strand, within CA265_RS25340 at 6160.574 kb on - strand, within CA265_RS25340 at 6160.710 kb on - strand, within CA265_RS25340 at 6160.720 kb on + strand at 6160.721 kb on - strand at 6160.766 kb on + strand at 6160.792 kb on - strand at 6160.831 kb on - strand at 6160.864 kb on + strand at 6160.870 kb on + strand at 6160.870 kb on + strand at 6160.871 kb on - strand at 6160.929 kb on + strand at 6161.153 kb on + strand, within CA265_RS25345 at 6161.182 kb on + strand, within CA265_RS25345 at 6161.183 kb on - strand, within CA265_RS25345 at 6161.183 kb on - strand, within CA265_RS25345 at 6161.193 kb on - strand, within CA265_RS25345 at 6161.325 kb on + strand at 6161.414 kb on + strand at 6161.456 kb on - strand at 6161.456 kb on - strand at 6161.470 kb on + strand at 6161.532 kb on - strand, within CA265_RS25350 at 6161.586 kb on + strand, within CA265_RS25350 at 6161.588 kb on + strand, within CA265_RS25350 at 6161.589 kb on - strand, within CA265_RS25350 at 6161.599 kb on - strand, within CA265_RS25350 at 6161.627 kb on - strand, within CA265_RS25350 at 6161.672 kb on - strand, within CA265_RS25350 at 6161.672 kb on - strand, within CA265_RS25350 at 6161.672 kb on - strand, within CA265_RS25350 at 6161.674 kb on + strand, within CA265_RS25350 at 6161.730 kb on - strand, within CA265_RS25350 at 6161.740 kb on + strand, within CA265_RS25350 at 6161.789 kb on - strand, within CA265_RS25350 at 6161.809 kb on - strand, within CA265_RS25350 at 6161.830 kb on + strand, within CA265_RS25350 at 6161.838 kb on - strand, within CA265_RS25350 at 6161.838 kb on - strand, within CA265_RS25350
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 6,158,444 + +0.2 6,158,456 + +1.1 6,158,510 + +0.5 6,158,511 - +0.2 6,158,512 + -1.0 6,158,579 + CA265_RS25330 0.14 -0.5 6,158,775 - CA265_RS25330 0.56 -0.8 6,158,816 + CA265_RS25330 0.65 +0.3 6,158,901 - CA265_RS25330 0.84 -0.4 6,159,007 + -0.4 6,159,055 + -0.5 6,159,170 + -0.0 6,159,170 + -0.7 6,159,170 + -0.7 6,159,170 + +0.7 6,159,171 - +0.2 6,159,200 + CA265_RS25335 0.12 -1.0 6,159,445 + -0.8 6,159,460 + -0.6 6,159,470 - -0.3 6,159,483 + -0.4 6,159,484 - +1.0 6,159,577 - -1.4 6,159,697 + CA265_RS25340 0.18 +0.3 6,159,716 + CA265_RS25340 0.20 -0.6 6,159,717 - CA265_RS25340 0.20 -1.2 6,159,737 - CA265_RS25340 0.21 -2.2 6,159,780 + CA265_RS25340 0.24 -1.4 6,159,780 + CA265_RS25340 0.24 -0.0 6,159,802 + CA265_RS25340 0.26 -1.2 6,159,807 + CA265_RS25340 0.26 -0.6 6,159,839 - CA265_RS25340 0.28 -0.6 6,159,888 + CA265_RS25340 0.32 -0.1 6,159,962 - CA265_RS25340 0.37 -0.6 6,159,975 - CA265_RS25340 0.38 -1.5 6,159,975 - CA265_RS25340 0.38 -0.9 6,159,994 + CA265_RS25340 0.39 -0.2 6,160,019 - CA265_RS25340 0.41 +0.5 6,160,022 + CA265_RS25340 0.41 -1.0 6,160,023 - CA265_RS25340 0.41 +0.2 6,160,030 - CA265_RS25340 0.42 -1.4 6,160,036 + CA265_RS25340 0.42 -0.5 6,160,078 - CA265_RS25340 0.45 +0.1 6,160,078 - CA265_RS25340 0.45 -0.9 6,160,097 - CA265_RS25340 0.46 -0.9 6,160,217 - CA265_RS25340 0.55 -0.7 6,160,217 - CA265_RS25340 0.55 +1.0 6,160,368 + CA265_RS25340 0.66 -1.2 6,160,373 - CA265_RS25340 0.66 -0.8 6,160,419 - CA265_RS25340 0.69 -1.0 6,160,427 + CA265_RS25340 0.70 -0.4 6,160,429 + CA265_RS25340 0.70 +1.3 6,160,429 + CA265_RS25340 0.70 -1.1 6,160,493 + CA265_RS25340 0.75 -2.4 6,160,497 + CA265_RS25340 0.75 -0.5 6,160,498 - CA265_RS25340 0.75 -0.6 6,160,508 - CA265_RS25340 0.76 -0.6 6,160,536 + CA265_RS25340 0.78 +0.3 6,160,549 + CA265_RS25340 0.78 -2.5 6,160,553 - CA265_RS25340 0.79 -0.4 6,160,563 - CA265_RS25340 0.79 -2.2 6,160,574 - CA265_RS25340 0.80 -0.9 6,160,710 - CA265_RS25340 0.90 +0.8 6,160,720 + +0.4 6,160,721 - +0.3 6,160,766 + +0.1 6,160,792 - -0.9 6,160,831 - -0.6 6,160,864 + +0.2 6,160,870 + +0.1 6,160,870 + +0.3 6,160,871 - +1.2 6,160,929 + +0.4 6,161,153 + CA265_RS25345 0.52 -0.5 6,161,182 + CA265_RS25345 0.59 +0.9 6,161,183 - CA265_RS25345 0.59 -1.0 6,161,183 - CA265_RS25345 0.59 -1.5 6,161,193 - CA265_RS25345 0.62 +0.2 6,161,325 + +0.1 6,161,414 + +0.3 6,161,456 - -0.1 6,161,456 - -0.3 6,161,470 + -1.0 6,161,532 - CA265_RS25350 0.12 +0.2 6,161,586 + CA265_RS25350 0.16 -0.6 6,161,588 + CA265_RS25350 0.16 -1.5 6,161,589 - CA265_RS25350 0.16 -0.9 6,161,599 - CA265_RS25350 0.17 +0.4 6,161,627 - CA265_RS25350 0.19 +0.3 6,161,672 - CA265_RS25350 0.23 -2.0 6,161,672 - CA265_RS25350 0.23 -1.5 6,161,672 - CA265_RS25350 0.23 +0.2 6,161,674 + CA265_RS25350 0.23 -0.5 6,161,730 - CA265_RS25350 0.27 -1.2 6,161,740 + CA265_RS25350 0.28 +0.8 6,161,789 - CA265_RS25350 0.32 -1.2 6,161,809 - CA265_RS25350 0.33 +0.1 6,161,830 + CA265_RS25350 0.35 -0.9 6,161,838 - CA265_RS25350 0.35 -0.9 6,161,838 - CA265_RS25350 0.35 -0.2
Or see this region's nucleotide sequence