Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS22820 and CA265_RS22825 are separated by 245 nucleotides CA265_RS22825 and CA265_RS22830 are separated by 2 nucleotides CA265_RS22830 and CA265_RS22835 are separated by 6 nucleotides
CA265_RS22820: CA265_RS22820 - flagellar motor protein MotB, at 5,582,924 to 5,584,267
_RS22820
CA265_RS22825: CA265_RS22825 - reactive intermediate/imine deaminase, at 5,584,513 to 5,584,893
_RS22825
CA265_RS22830: CA265_RS22830 - permease, at 5,584,896 to 5,585,798
_RS22830
CA265_RS22835: CA265_RS22835 - ATP-dependent DNA helicase RecG, at 5,585,805 to 5,587,910
_RS22835
Position (kb)
5584
5585 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5583.555 kb on + strand, within CA265_RS22820 at 5583.561 kb on - strand, within CA265_RS22820 at 5583.619 kb on + strand, within CA265_RS22820 at 5583.635 kb on + strand, within CA265_RS22820 at 5583.710 kb on - strand, within CA265_RS22820 at 5583.712 kb on + strand, within CA265_RS22820 at 5583.716 kb on + strand, within CA265_RS22820 at 5583.815 kb on - strand, within CA265_RS22820 at 5583.872 kb on - strand, within CA265_RS22820 at 5583.935 kb on - strand, within CA265_RS22820 at 5584.012 kb on + strand, within CA265_RS22820 at 5584.017 kb on + strand, within CA265_RS22820 at 5584.094 kb on - strand, within CA265_RS22820 at 5584.094 kb on - strand, within CA265_RS22820 at 5584.108 kb on + strand, within CA265_RS22820 at 5584.141 kb on - strand at 5584.174 kb on + strand at 5584.175 kb on - strand at 5584.175 kb on - strand at 5584.175 kb on - strand at 5584.175 kb on - strand at 5584.177 kb on + strand at 5584.177 kb on + strand at 5584.183 kb on + strand at 5584.183 kb on + strand at 5584.210 kb on + strand at 5584.211 kb on - strand at 5584.266 kb on - strand at 5584.269 kb on - strand at 5584.278 kb on + strand at 5584.318 kb on + strand at 5584.353 kb on + strand at 5584.368 kb on + strand at 5584.369 kb on - strand at 5584.391 kb on + strand at 5584.403 kb on + strand at 5584.467 kb on - strand at 5584.501 kb on - strand at 5584.525 kb on - strand at 5584.561 kb on + strand, within CA265_RS22825 at 5584.564 kb on - strand, within CA265_RS22825 at 5584.574 kb on + strand, within CA265_RS22825 at 5584.599 kb on - strand, within CA265_RS22825 at 5584.618 kb on - strand, within CA265_RS22825 at 5584.637 kb on + strand, within CA265_RS22825 at 5584.648 kb on - strand, within CA265_RS22825 at 5584.862 kb on + strand at 5584.863 kb on - strand at 5584.874 kb on + strand at 5584.886 kb on + strand at 5584.887 kb on - strand at 5584.942 kb on - strand at 5584.945 kb on + strand at 5584.946 kb on - strand at 5584.981 kb on + strand at 5585.013 kb on + strand, within CA265_RS22830 at 5585.014 kb on - strand, within CA265_RS22830 at 5585.096 kb on - strand, within CA265_RS22830 at 5585.098 kb on + strand, within CA265_RS22830 at 5585.191 kb on - strand, within CA265_RS22830 at 5585.201 kb on + strand, within CA265_RS22830 at 5585.271 kb on - strand, within CA265_RS22830 at 5585.291 kb on - strand, within CA265_RS22830 at 5585.307 kb on - strand, within CA265_RS22830 at 5585.327 kb on + strand, within CA265_RS22830 at 5585.329 kb on + strand, within CA265_RS22830 at 5585.341 kb on + strand, within CA265_RS22830 at 5585.342 kb on - strand, within CA265_RS22830 at 5585.405 kb on - strand, within CA265_RS22830 at 5585.406 kb on + strand, within CA265_RS22830 at 5585.406 kb on + strand, within CA265_RS22830 at 5585.406 kb on + strand, within CA265_RS22830 at 5585.407 kb on - strand, within CA265_RS22830 at 5585.407 kb on - strand, within CA265_RS22830 at 5585.407 kb on - strand, within CA265_RS22830 at 5585.437 kb on + strand, within CA265_RS22830 at 5585.549 kb on + strand, within CA265_RS22830 at 5585.552 kb on - strand, within CA265_RS22830 at 5585.594 kb on - strand, within CA265_RS22830 at 5585.669 kb on + strand, within CA265_RS22830 at 5585.670 kb on - strand, within CA265_RS22830 at 5585.771 kb on - strand at 5585.821 kb on + strand at 5585.821 kb on + strand at 5585.826 kb on - strand at 5585.826 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 5,583,555 + CA265_RS22820 0.47 -2.6 5,583,561 - CA265_RS22820 0.47 -0.8 5,583,619 + CA265_RS22820 0.52 -3.4 5,583,635 + CA265_RS22820 0.53 -0.8 5,583,710 - CA265_RS22820 0.58 -1.0 5,583,712 + CA265_RS22820 0.59 -1.4 5,583,716 + CA265_RS22820 0.59 -2.0 5,583,815 - CA265_RS22820 0.66 -2.4 5,583,872 - CA265_RS22820 0.71 -1.7 5,583,935 - CA265_RS22820 0.75 -3.5 5,584,012 + CA265_RS22820 0.81 -2.5 5,584,017 + CA265_RS22820 0.81 -1.1 5,584,094 - CA265_RS22820 0.87 -0.8 5,584,094 - CA265_RS22820 0.87 -3.3 5,584,108 + CA265_RS22820 0.88 -0.8 5,584,141 - -1.8 5,584,174 + -1.4 5,584,175 - -0.8 5,584,175 - -1.9 5,584,175 - -0.0 5,584,175 - -1.5 5,584,177 + -0.0 5,584,177 + -1.1 5,584,183 + -0.5 5,584,183 + -1.4 5,584,210 + -0.0 5,584,211 - -2.5 5,584,266 - -0.7 5,584,269 - -3.0 5,584,278 + -1.8 5,584,318 + -0.4 5,584,353 + -1.1 5,584,368 + +0.4 5,584,369 - -0.6 5,584,391 + +0.1 5,584,403 + -0.3 5,584,467 - -3.5 5,584,501 - -2.5 5,584,525 - -3.6 5,584,561 + CA265_RS22825 0.13 +0.3 5,584,564 - CA265_RS22825 0.13 -1.8 5,584,574 + CA265_RS22825 0.16 +0.0 5,584,599 - CA265_RS22825 0.23 -1.8 5,584,618 - CA265_RS22825 0.28 -0.1 5,584,637 + CA265_RS22825 0.33 -0.8 5,584,648 - CA265_RS22825 0.35 -1.7 5,584,862 + -0.0 5,584,863 - -1.4 5,584,874 + +1.0 5,584,886 + +0.6 5,584,887 - +1.3 5,584,942 - -1.0 5,584,945 + -0.0 5,584,946 - -0.3 5,584,981 + -0.0 5,585,013 + CA265_RS22830 0.13 +0.3 5,585,014 - CA265_RS22830 0.13 -1.5 5,585,096 - CA265_RS22830 0.22 -1.1 5,585,098 + CA265_RS22830 0.22 +0.3 5,585,191 - CA265_RS22830 0.33 +0.1 5,585,201 + CA265_RS22830 0.34 +0.7 5,585,271 - CA265_RS22830 0.42 -1.2 5,585,291 - CA265_RS22830 0.44 -2.1 5,585,307 - CA265_RS22830 0.46 -0.3 5,585,327 + CA265_RS22830 0.48 +0.1 5,585,329 + CA265_RS22830 0.48 -0.8 5,585,341 + CA265_RS22830 0.49 -0.6 5,585,342 - CA265_RS22830 0.49 -1.0 5,585,405 - CA265_RS22830 0.56 +0.6 5,585,406 + CA265_RS22830 0.56 +1.1 5,585,406 + CA265_RS22830 0.56 -1.0 5,585,406 + CA265_RS22830 0.56 -2.4 5,585,407 - CA265_RS22830 0.57 -0.3 5,585,407 - CA265_RS22830 0.57 -1.9 5,585,407 - CA265_RS22830 0.57 +1.3 5,585,437 + CA265_RS22830 0.60 +0.9 5,585,549 + CA265_RS22830 0.72 +0.7 5,585,552 - CA265_RS22830 0.73 +1.1 5,585,594 - CA265_RS22830 0.77 -1.0 5,585,669 + CA265_RS22830 0.86 -0.3 5,585,670 - CA265_RS22830 0.86 -1.0 5,585,771 - +2.3 5,585,821 + -0.7 5,585,821 + -0.5 5,585,826 - -0.5 5,585,826 - -1.4
Or see this region's nucleotide sequence