Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS13265 and CA265_RS13270 are separated by 71 nucleotides CA265_RS13270 and CA265_RS13275 are separated by 186 nucleotides CA265_RS13275 and CA265_RS13280 are separated by 362 nucleotides CA265_RS13280 and CA265_RS13285 are separated by 9 nucleotides
CA265_RS13265: CA265_RS13265 - DNA-binding protein, at 3,296,172 to 3,296,495
_RS13265
CA265_RS13270: CA265_RS13270 - hypothetical protein, at 3,296,567 to 3,297,031
_RS13270
CA265_RS13275: CA265_RS13275 - redox-regulated ATPase YchF, at 3,297,218 to 3,298,318
_RS13275
CA265_RS13280: CA265_RS13280 - hydrolase, at 3,298,681 to 3,299,295
_RS13280
CA265_RS13285: CA265_RS13285 - magnesium transporter, at 3,299,305 to 3,300,681
_RS13285
Position (kb)
3297
3298
3299 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3296.241 kb on + strand, within CA265_RS13265 at 3296.262 kb on - strand, within CA265_RS13265 at 3296.264 kb on + strand, within CA265_RS13265 at 3296.278 kb on - strand, within CA265_RS13265 at 3296.348 kb on - strand, within CA265_RS13265 at 3296.443 kb on - strand, within CA265_RS13265 at 3296.557 kb on - strand at 3296.558 kb on + strand at 3296.559 kb on - strand at 3296.559 kb on - strand at 3296.579 kb on + strand at 3296.631 kb on - strand, within CA265_RS13270 at 3296.637 kb on + strand, within CA265_RS13270 at 3296.638 kb on + strand, within CA265_RS13270 at 3296.790 kb on + strand, within CA265_RS13270 at 3296.797 kb on + strand, within CA265_RS13270 at 3296.835 kb on - strand, within CA265_RS13270 at 3296.891 kb on + strand, within CA265_RS13270 at 3296.948 kb on - strand, within CA265_RS13270 at 3296.954 kb on - strand, within CA265_RS13270 at 3296.954 kb on - strand, within CA265_RS13270 at 3297.033 kb on + strand at 3297.034 kb on - strand at 3297.034 kb on - strand at 3297.034 kb on - strand at 3297.043 kb on + strand at 3297.053 kb on + strand at 3297.064 kb on - strand at 3297.069 kb on - strand at 3297.069 kb on - strand at 3297.084 kb on + strand at 3297.085 kb on - strand at 3297.116 kb on + strand at 3297.116 kb on + strand at 3297.203 kb on - strand at 3297.213 kb on - strand at 3297.213 kb on - strand at 3297.225 kb on + strand at 3297.235 kb on + strand at 3297.237 kb on + strand at 3297.238 kb on - strand at 3297.282 kb on + strand at 3297.349 kb on + strand, within CA265_RS13275 at 3297.349 kb on + strand, within CA265_RS13275 at 3297.416 kb on - strand, within CA265_RS13275 at 3297.434 kb on - strand, within CA265_RS13275 at 3297.529 kb on - strand, within CA265_RS13275 at 3297.552 kb on + strand, within CA265_RS13275 at 3297.599 kb on - strand, within CA265_RS13275 at 3297.605 kb on - strand, within CA265_RS13275 at 3297.620 kb on - strand, within CA265_RS13275 at 3297.704 kb on + strand, within CA265_RS13275 at 3297.704 kb on + strand, within CA265_RS13275 at 3297.745 kb on + strand, within CA265_RS13275 at 3297.812 kb on - strand, within CA265_RS13275 at 3297.830 kb on + strand, within CA265_RS13275 at 3297.831 kb on - strand, within CA265_RS13275 at 3297.886 kb on - strand, within CA265_RS13275 at 3297.967 kb on + strand, within CA265_RS13275 at 3297.972 kb on - strand, within CA265_RS13275 at 3298.049 kb on - strand, within CA265_RS13275 at 3298.071 kb on - strand, within CA265_RS13275 at 3298.215 kb on + strand at 3298.216 kb on - strand at 3298.216 kb on - strand at 3298.217 kb on - strand at 3298.252 kb on - strand at 3298.257 kb on + strand at 3298.258 kb on - strand at 3298.258 kb on - strand at 3298.275 kb on + strand at 3298.430 kb on + strand at 3298.573 kb on + strand at 3298.574 kb on - strand at 3298.583 kb on + strand at 3298.583 kb on + strand at 3298.587 kb on + strand at 3298.587 kb on + strand at 3298.588 kb on - strand at 3298.635 kb on + strand at 3298.635 kb on + strand at 3298.668 kb on + strand at 3298.669 kb on - strand at 3298.675 kb on + strand at 3298.682 kb on + strand at 3298.683 kb on - strand at 3298.698 kb on - strand at 3298.711 kb on + strand at 3298.757 kb on - strand, within CA265_RS13280 at 3298.799 kb on + strand, within CA265_RS13280 at 3298.799 kb on + strand, within CA265_RS13280 at 3298.800 kb on - strand, within CA265_RS13280 at 3298.800 kb on - strand, within CA265_RS13280 at 3298.800 kb on - strand, within CA265_RS13280 at 3298.986 kb on + strand, within CA265_RS13280 at 3298.988 kb on + strand, within CA265_RS13280 at 3298.989 kb on - strand, within CA265_RS13280 at 3298.989 kb on - strand, within CA265_RS13280 at 3299.103 kb on + strand, within CA265_RS13280 at 3299.129 kb on - strand, within CA265_RS13280 at 3299.144 kb on + strand, within CA265_RS13280 at 3299.144 kb on + strand, within CA265_RS13280 at 3299.145 kb on - strand, within CA265_RS13280 at 3299.145 kb on - strand, within CA265_RS13280 at 3299.145 kb on - strand, within CA265_RS13280 at 3299.149 kb on - strand, within CA265_RS13280 at 3299.158 kb on - strand, within CA265_RS13280 at 3299.281 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 3,296,241 + CA265_RS13265 0.21 +0.7 3,296,262 - CA265_RS13265 0.28 -0.6 3,296,264 + CA265_RS13265 0.28 -2.0 3,296,278 - CA265_RS13265 0.33 -0.8 3,296,348 - CA265_RS13265 0.54 -1.9 3,296,443 - CA265_RS13265 0.84 -0.5 3,296,557 - +0.1 3,296,558 + +0.2 3,296,559 - +1.4 3,296,559 - -0.2 3,296,579 + -0.6 3,296,631 - CA265_RS13270 0.14 -0.5 3,296,637 + CA265_RS13270 0.15 -0.2 3,296,638 + CA265_RS13270 0.15 +0.4 3,296,790 + CA265_RS13270 0.48 -0.9 3,296,797 + CA265_RS13270 0.49 -0.2 3,296,835 - CA265_RS13270 0.58 +0.2 3,296,891 + CA265_RS13270 0.70 +1.6 3,296,948 - CA265_RS13270 0.82 +1.8 3,296,954 - CA265_RS13270 0.83 +0.4 3,296,954 - CA265_RS13270 0.83 -1.2 3,297,033 + -1.3 3,297,034 - -0.4 3,297,034 - -0.4 3,297,034 - -0.4 3,297,043 + -1.5 3,297,053 + -0.2 3,297,064 - -0.9 3,297,069 - -0.2 3,297,069 - +0.9 3,297,084 + +1.6 3,297,085 - -1.3 3,297,116 + +0.7 3,297,116 + +0.3 3,297,203 - -0.6 3,297,213 - +0.9 3,297,213 - -2.0 3,297,225 + -1.6 3,297,235 + -1.0 3,297,237 + +1.8 3,297,238 - -0.8 3,297,282 + -0.6 3,297,349 + CA265_RS13275 0.12 -0.7 3,297,349 + CA265_RS13275 0.12 -1.4 3,297,416 - CA265_RS13275 0.18 -1.5 3,297,434 - CA265_RS13275 0.20 -2.1 3,297,529 - CA265_RS13275 0.28 -1.6 3,297,552 + CA265_RS13275 0.30 -0.3 3,297,599 - CA265_RS13275 0.35 -0.7 3,297,605 - CA265_RS13275 0.35 -0.6 3,297,620 - CA265_RS13275 0.37 +0.6 3,297,704 + CA265_RS13275 0.44 +0.2 3,297,704 + CA265_RS13275 0.44 +0.3 3,297,745 + CA265_RS13275 0.48 -0.6 3,297,812 - CA265_RS13275 0.54 -0.2 3,297,830 + CA265_RS13275 0.56 -0.2 3,297,831 - CA265_RS13275 0.56 -1.1 3,297,886 - CA265_RS13275 0.61 +1.0 3,297,967 + CA265_RS13275 0.68 -0.8 3,297,972 - CA265_RS13275 0.68 +0.5 3,298,049 - CA265_RS13275 0.75 -0.8 3,298,071 - CA265_RS13275 0.77 -1.7 3,298,215 + +0.0 3,298,216 - +0.6 3,298,216 - -0.2 3,298,217 - +0.8 3,298,252 - +0.1 3,298,257 + +1.0 3,298,258 - -0.7 3,298,258 - -2.7 3,298,275 + +0.7 3,298,430 + +0.0 3,298,573 + -0.1 3,298,574 - -0.1 3,298,583 + +0.2 3,298,583 + +1.0 3,298,587 + -0.0 3,298,587 + -0.5 3,298,588 - -0.6 3,298,635 + +0.1 3,298,635 + -0.6 3,298,668 + +2.6 3,298,669 - +0.6 3,298,675 + -0.5 3,298,682 + -0.5 3,298,683 - +0.2 3,298,698 - +0.8 3,298,711 + -0.5 3,298,757 - CA265_RS13280 0.12 -0.3 3,298,799 + CA265_RS13280 0.19 -1.0 3,298,799 + CA265_RS13280 0.19 -0.0 3,298,800 - CA265_RS13280 0.19 +0.4 3,298,800 - CA265_RS13280 0.19 +0.5 3,298,800 - CA265_RS13280 0.19 -0.6 3,298,986 + CA265_RS13280 0.50 -0.8 3,298,988 + CA265_RS13280 0.50 +1.3 3,298,989 - CA265_RS13280 0.50 +0.3 3,298,989 - CA265_RS13280 0.50 -0.4 3,299,103 + CA265_RS13280 0.69 -0.1 3,299,129 - CA265_RS13280 0.73 +1.2 3,299,144 + CA265_RS13280 0.75 +1.5 3,299,144 + CA265_RS13280 0.75 -0.9 3,299,145 - CA265_RS13280 0.75 +0.4 3,299,145 - CA265_RS13280 0.75 +0.4 3,299,145 - CA265_RS13280 0.75 -0.0 3,299,149 - CA265_RS13280 0.76 -0.4 3,299,158 - CA265_RS13280 0.78 +0.6 3,299,281 - -0.0
Or see this region's nucleotide sequence