Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS12815 and CA265_RS12820 are separated by 167 nucleotides CA265_RS12820 and CA265_RS12825 are separated by 547 nucleotides
CA265_RS12815: CA265_RS12815 - hypothetical protein, at 3,192,720 to 3,195,497
_RS12815
CA265_RS12820: CA265_RS12820 - hypothetical protein, at 3,195,665 to 3,196,501
_RS12820
CA265_RS12825: CA265_RS12825 - hypothetical protein, at 3,197,049 to 3,197,492
_RS12825
Position (kb)
3195
3196
3197 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3194.666 kb on + strand, within CA265_RS12815 at 3194.682 kb on + strand, within CA265_RS12815 at 3194.796 kb on - strand, within CA265_RS12815 at 3194.802 kb on - strand, within CA265_RS12815 at 3194.805 kb on - strand, within CA265_RS12815 at 3194.816 kb on + strand, within CA265_RS12815 at 3194.816 kb on + strand, within CA265_RS12815 at 3194.817 kb on - strand, within CA265_RS12815 at 3194.884 kb on + strand, within CA265_RS12815 at 3194.991 kb on + strand, within CA265_RS12815 at 3195.032 kb on + strand, within CA265_RS12815 at 3195.092 kb on + strand, within CA265_RS12815 at 3195.115 kb on - strand, within CA265_RS12815 at 3195.210 kb on + strand, within CA265_RS12815 at 3195.268 kb on - strand at 3195.268 kb on - strand at 3195.269 kb on + strand at 3195.270 kb on - strand at 3195.290 kb on + strand at 3195.290 kb on + strand at 3195.293 kb on + strand at 3195.294 kb on - strand at 3195.313 kb on + strand at 3195.367 kb on - strand at 3195.387 kb on + strand at 3195.439 kb on + strand at 3195.439 kb on + strand at 3195.606 kb on + strand at 3195.627 kb on + strand at 3195.627 kb on + strand at 3195.627 kb on + strand at 3195.646 kb on + strand at 3195.723 kb on - strand at 3195.799 kb on + strand, within CA265_RS12820 at 3195.837 kb on + strand, within CA265_RS12820 at 3195.838 kb on - strand, within CA265_RS12820 at 3195.848 kb on + strand, within CA265_RS12820 at 3195.849 kb on - strand, within CA265_RS12820 at 3195.889 kb on - strand, within CA265_RS12820 at 3195.939 kb on - strand, within CA265_RS12820 at 3195.975 kb on + strand, within CA265_RS12820 at 3195.975 kb on + strand, within CA265_RS12820 at 3195.980 kb on - strand, within CA265_RS12820 at 3196.011 kb on - strand, within CA265_RS12820 at 3196.011 kb on - strand, within CA265_RS12820 at 3196.043 kb on + strand, within CA265_RS12820 at 3196.044 kb on - strand, within CA265_RS12820 at 3196.064 kb on - strand at 3196.106 kb on - strand, within CA265_RS12820 at 3196.145 kb on - strand, within CA265_RS12820 at 3196.145 kb on - strand, within CA265_RS12820 at 3196.153 kb on - strand, within CA265_RS12820 at 3196.172 kb on - strand, within CA265_RS12820 at 3196.227 kb on - strand, within CA265_RS12820 at 3196.311 kb on - strand, within CA265_RS12820 at 3196.423 kb on + strand at 3196.439 kb on - strand at 3196.493 kb on + strand at 3196.499 kb on + strand at 3196.524 kb on + strand at 3196.563 kb on + strand at 3196.595 kb on - strand at 3196.595 kb on - strand at 3196.595 kb on - strand at 3196.651 kb on - strand at 3196.703 kb on + strand at 3196.935 kb on + strand at 3196.935 kb on + strand at 3196.935 kb on + strand at 3196.936 kb on - strand at 3196.945 kb on + strand at 3196.945 kb on + strand at 3196.945 kb on + strand at 3197.051 kb on - strand at 3197.094 kb on + strand, within CA265_RS12825 at 3197.101 kb on - strand, within CA265_RS12825 at 3197.164 kb on - strand, within CA265_RS12825 at 3197.211 kb on + strand, within CA265_RS12825 at 3197.278 kb on + strand, within CA265_RS12825 at 3197.420 kb on + strand, within CA265_RS12825 at 3197.436 kb on - strand, within CA265_RS12825 at 3197.486 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 3,194,666 + CA265_RS12815 0.70 -0.8 3,194,682 + CA265_RS12815 0.71 -0.1 3,194,796 - CA265_RS12815 0.75 +0.3 3,194,802 - CA265_RS12815 0.75 +0.3 3,194,805 - CA265_RS12815 0.75 -0.2 3,194,816 + CA265_RS12815 0.75 +0.1 3,194,816 + CA265_RS12815 0.75 +1.6 3,194,817 - CA265_RS12815 0.75 +0.6 3,194,884 + CA265_RS12815 0.78 -0.0 3,194,991 + CA265_RS12815 0.82 -0.4 3,195,032 + CA265_RS12815 0.83 +0.7 3,195,092 + CA265_RS12815 0.85 -0.4 3,195,115 - CA265_RS12815 0.86 -0.0 3,195,210 + CA265_RS12815 0.90 +0.2 3,195,268 - +0.2 3,195,268 - +0.6 3,195,269 + -0.2 3,195,270 - +0.6 3,195,290 + -0.3 3,195,290 + +0.2 3,195,293 + -1.7 3,195,294 - +0.6 3,195,313 + +1.2 3,195,367 - -0.5 3,195,387 + -0.3 3,195,439 + -0.8 3,195,439 + -0.5 3,195,606 + -1.1 3,195,627 + +0.4 3,195,627 + +0.1 3,195,627 + -0.8 3,195,646 + -0.2 3,195,723 - +2.0 3,195,799 + CA265_RS12820 0.16 -0.3 3,195,837 + CA265_RS12820 0.21 -0.0 3,195,838 - CA265_RS12820 0.21 +1.6 3,195,848 + CA265_RS12820 0.22 -1.4 3,195,849 - CA265_RS12820 0.22 +2.1 3,195,889 - CA265_RS12820 0.27 +3.3 3,195,939 - CA265_RS12820 0.33 +1.4 3,195,975 + CA265_RS12820 0.37 +1.7 3,195,975 + CA265_RS12820 0.37 -0.8 3,195,980 - CA265_RS12820 0.38 +0.9 3,196,011 - CA265_RS12820 0.41 +1.8 3,196,011 - CA265_RS12820 0.41 +0.9 3,196,043 + CA265_RS12820 0.45 +0.6 3,196,044 - CA265_RS12820 0.45 +0.9 3,196,064 - +2.1 3,196,106 - CA265_RS12820 0.53 +2.2 3,196,145 - CA265_RS12820 0.57 +1.5 3,196,145 - CA265_RS12820 0.57 +1.4 3,196,153 - CA265_RS12820 0.58 +1.3 3,196,172 - CA265_RS12820 0.61 +1.7 3,196,227 - CA265_RS12820 0.67 +1.7 3,196,311 - CA265_RS12820 0.77 +1.8 3,196,423 + -0.9 3,196,439 - +0.7 3,196,493 + -0.3 3,196,499 + -0.1 3,196,524 + +0.9 3,196,563 + -0.1 3,196,595 - +2.3 3,196,595 - +1.1 3,196,595 - +0.7 3,196,651 - -0.3 3,196,703 + -0.6 3,196,935 + -0.6 3,196,935 + -0.9 3,196,935 + -1.5 3,196,936 - +1.1 3,196,945 + -1.4 3,196,945 + -0.9 3,196,945 + -0.1 3,197,051 - +0.3 3,197,094 + CA265_RS12825 0.10 -0.3 3,197,101 - CA265_RS12825 0.12 +0.2 3,197,164 - CA265_RS12825 0.26 +0.4 3,197,211 + CA265_RS12825 0.36 -0.8 3,197,278 + CA265_RS12825 0.52 -0.5 3,197,420 + CA265_RS12825 0.84 -1.0 3,197,436 - CA265_RS12825 0.87 +0.9 3,197,486 - +0.7
Or see this region's nucleotide sequence