Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS12320 and CA265_RS12325 are separated by 375 nucleotides CA265_RS12325 and CA265_RS12330 are separated by 163 nucleotides CA265_RS12330 and CA265_RS12335 are separated by 64 nucleotides
CA265_RS12320: CA265_RS12320 - hypothetical protein, at 3,071,993 to 3,072,265
_RS12320
CA265_RS12325: CA265_RS12325 - hypothetical protein, at 3,072,641 to 3,073,063
_RS12325
CA265_RS12330: CA265_RS12330 - response regulator, at 3,073,227 to 3,073,598
_RS12330
CA265_RS12335: CA265_RS12335 - hypothetical protein, at 3,073,663 to 3,076,455
_RS12335
Position (kb)
3073
3074 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3072.289 kb on + strand at 3072.289 kb on + strand at 3072.520 kb on - strand at 3072.586 kb on + strand at 3072.587 kb on - strand at 3072.609 kb on + strand at 3072.652 kb on - strand at 3072.683 kb on - strand at 3072.683 kb on - strand at 3072.735 kb on + strand, within CA265_RS12325 at 3072.735 kb on + strand, within CA265_RS12325 at 3072.766 kb on + strand, within CA265_RS12325 at 3072.779 kb on + strand, within CA265_RS12325 at 3072.780 kb on - strand, within CA265_RS12325 at 3072.780 kb on - strand, within CA265_RS12325 at 3072.820 kb on + strand, within CA265_RS12325 at 3072.821 kb on - strand, within CA265_RS12325 at 3072.821 kb on - strand, within CA265_RS12325 at 3072.834 kb on - strand, within CA265_RS12325 at 3072.878 kb on - strand, within CA265_RS12325 at 3072.892 kb on + strand, within CA265_RS12325 at 3072.899 kb on - strand, within CA265_RS12325 at 3072.963 kb on + strand, within CA265_RS12325 at 3072.964 kb on - strand, within CA265_RS12325 at 3072.993 kb on + strand, within CA265_RS12325 at 3072.994 kb on - strand, within CA265_RS12325 at 3072.995 kb on + strand, within CA265_RS12325 at 3073.001 kb on - strand at 3073.065 kb on + strand at 3073.066 kb on - strand at 3073.099 kb on - strand at 3073.109 kb on + strand at 3073.109 kb on + strand at 3073.109 kb on + strand at 3073.121 kb on - strand at 3073.136 kb on + strand at 3073.216 kb on + strand at 3073.216 kb on + strand at 3073.218 kb on + strand at 3073.218 kb on + strand at 3073.219 kb on - strand at 3073.219 kb on - strand at 3073.238 kb on + strand at 3073.360 kb on + strand, within CA265_RS12330 at 3073.406 kb on + strand, within CA265_RS12330 at 3073.533 kb on - strand, within CA265_RS12330 at 3073.596 kb on + strand at 3073.611 kb on + strand at 3073.784 kb on + strand at 3073.828 kb on + strand at 3074.010 kb on - strand, within CA265_RS12335 at 3074.049 kb on + strand, within CA265_RS12335 at 3074.268 kb on - strand, within CA265_RS12335 at 3074.369 kb on + strand, within CA265_RS12335 at 3074.370 kb on - strand, within CA265_RS12335 at 3074.411 kb on + strand, within CA265_RS12335 at 3074.412 kb on - strand, within CA265_RS12335 at 3074.428 kb on - strand, within CA265_RS12335
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 3,072,289 + +0.8 3,072,289 + +1.1 3,072,520 - +0.7 3,072,586 + -0.6 3,072,587 - -0.5 3,072,609 + -2.6 3,072,652 - -0.8 3,072,683 - +0.2 3,072,683 - -0.7 3,072,735 + CA265_RS12325 0.22 -2.0 3,072,735 + CA265_RS12325 0.22 -0.2 3,072,766 + CA265_RS12325 0.30 +2.7 3,072,779 + CA265_RS12325 0.33 +1.2 3,072,780 - CA265_RS12325 0.33 +0.0 3,072,780 - CA265_RS12325 0.33 +1.0 3,072,820 + CA265_RS12325 0.42 +1.0 3,072,821 - CA265_RS12325 0.43 +0.0 3,072,821 - CA265_RS12325 0.43 -1.2 3,072,834 - CA265_RS12325 0.46 -0.3 3,072,878 - CA265_RS12325 0.56 +0.4 3,072,892 + CA265_RS12325 0.59 -0.4 3,072,899 - CA265_RS12325 0.61 -0.6 3,072,963 + CA265_RS12325 0.76 -0.3 3,072,964 - CA265_RS12325 0.76 +0.3 3,072,993 + CA265_RS12325 0.83 +1.1 3,072,994 - CA265_RS12325 0.83 -0.5 3,072,995 + CA265_RS12325 0.84 +0.9 3,073,001 - +1.7 3,073,065 + +0.0 3,073,066 - +0.6 3,073,099 - +0.5 3,073,109 + -0.2 3,073,109 + -0.3 3,073,109 + +2.0 3,073,121 - -0.1 3,073,136 + -0.4 3,073,216 + -0.3 3,073,216 + +0.6 3,073,218 + +1.4 3,073,218 + -0.1 3,073,219 - -1.1 3,073,219 - +0.4 3,073,238 + -0.5 3,073,360 + CA265_RS12330 0.36 -0.6 3,073,406 + CA265_RS12330 0.48 -0.4 3,073,533 - CA265_RS12330 0.82 -0.1 3,073,596 + -0.5 3,073,611 + -0.3 3,073,784 + -0.6 3,073,828 + +1.3 3,074,010 - CA265_RS12335 0.12 +1.5 3,074,049 + CA265_RS12335 0.14 +1.2 3,074,268 - CA265_RS12335 0.22 -0.0 3,074,369 + CA265_RS12335 0.25 -0.3 3,074,370 - CA265_RS12335 0.25 +0.1 3,074,411 + CA265_RS12335 0.27 +0.6 3,074,412 - CA265_RS12335 0.27 +0.6 3,074,428 - CA265_RS12335 0.27 +0.9
Or see this region's nucleotide sequence