Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS08440 and CA265_RS08445 are separated by 76 nucleotides CA265_RS08445 and CA265_RS08450 are separated by 166 nucleotides CA265_RS08450 and CA265_RS08455 are separated by 1 nucleotides
CA265_RS08440: CA265_RS08440 - polysaccharide lyase, at 2,103,254 to 2,104,885
_RS08440
CA265_RS08445: CA265_RS08445 - endonuclease, at 2,104,962 to 2,105,264
_RS08445
CA265_RS08450: CA265_RS08450 - pectate lyase, at 2,105,431 to 2,108,592
_RS08450
CA265_RS08455: CA265_RS08455 - glycoside hydrolase, at 2,108,594 to 2,109,994
_RS08455
Position (kb)
2105
2106
2107
2108
2109 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2104.456 kb on - strand, within CA265_RS08440 at 2104.459 kb on + strand, within CA265_RS08440 at 2104.459 kb on + strand, within CA265_RS08440 at 2104.558 kb on + strand, within CA265_RS08440 at 2104.559 kb on - strand, within CA265_RS08440 at 2104.628 kb on + strand, within CA265_RS08440 at 2104.628 kb on + strand, within CA265_RS08440 at 2104.632 kb on + strand, within CA265_RS08440 at 2104.658 kb on + strand at 2104.780 kb on + strand at 2104.804 kb on + strand at 2104.844 kb on + strand at 2104.897 kb on - strand at 2104.897 kb on - strand at 2104.899 kb on - strand at 2104.958 kb on + strand at 2104.958 kb on + strand at 2104.983 kb on + strand at 2104.984 kb on - strand at 2105.038 kb on + strand, within CA265_RS08445 at 2105.298 kb on + strand at 2105.308 kb on + strand at 2105.308 kb on + strand at 2105.309 kb on - strand at 2105.384 kb on + strand at 2105.385 kb on - strand at 2105.385 kb on - strand at 2105.430 kb on - strand at 2105.450 kb on + strand at 2105.451 kb on - strand at 2105.468 kb on + strand at 2105.490 kb on - strand at 2105.590 kb on - strand at 2105.638 kb on + strand at 2105.738 kb on - strand at 2105.803 kb on + strand, within CA265_RS08450 at 2105.803 kb on + strand, within CA265_RS08450 at 2105.803 kb on + strand, within CA265_RS08450 at 2105.803 kb on + strand, within CA265_RS08450 at 2105.804 kb on - strand, within CA265_RS08450 at 2105.907 kb on + strand, within CA265_RS08450 at 2105.908 kb on - strand, within CA265_RS08450 at 2105.908 kb on - strand, within CA265_RS08450 at 2105.973 kb on + strand, within CA265_RS08450 at 2105.974 kb on - strand, within CA265_RS08450 at 2105.978 kb on - strand, within CA265_RS08450 at 2105.978 kb on - strand, within CA265_RS08450 at 2105.978 kb on - strand, within CA265_RS08450 at 2106.176 kb on + strand, within CA265_RS08450 at 2106.459 kb on + strand, within CA265_RS08450 at 2106.475 kb on - strand, within CA265_RS08450 at 2106.520 kb on - strand, within CA265_RS08450 at 2106.572 kb on - strand, within CA265_RS08450 at 2106.577 kb on + strand, within CA265_RS08450 at 2106.640 kb on + strand, within CA265_RS08450 at 2106.704 kb on + strand, within CA265_RS08450 at 2106.786 kb on - strand, within CA265_RS08450 at 2106.799 kb on - strand, within CA265_RS08450 at 2106.925 kb on + strand, within CA265_RS08450 at 2106.926 kb on - strand, within CA265_RS08450 at 2106.954 kb on + strand, within CA265_RS08450 at 2106.955 kb on - strand, within CA265_RS08450 at 2107.025 kb on + strand, within CA265_RS08450 at 2107.054 kb on - strand, within CA265_RS08450 at 2107.104 kb on + strand, within CA265_RS08450 at 2107.138 kb on - strand, within CA265_RS08450 at 2107.143 kb on + strand, within CA265_RS08450 at 2107.164 kb on + strand, within CA265_RS08450 at 2107.283 kb on - strand, within CA265_RS08450 at 2107.347 kb on - strand, within CA265_RS08450 at 2107.347 kb on - strand, within CA265_RS08450 at 2107.368 kb on + strand, within CA265_RS08450 at 2107.369 kb on - strand at 2107.455 kb on + strand, within CA265_RS08450 at 2107.549 kb on + strand, within CA265_RS08450 at 2107.550 kb on - strand, within CA265_RS08450 at 2107.566 kb on + strand, within CA265_RS08450 at 2107.566 kb on + strand, within CA265_RS08450 at 2107.584 kb on + strand, within CA265_RS08450 at 2107.585 kb on - strand, within CA265_RS08450 at 2107.589 kb on + strand, within CA265_RS08450 at 2107.660 kb on - strand, within CA265_RS08450 at 2107.681 kb on + strand, within CA265_RS08450 at 2107.682 kb on - strand, within CA265_RS08450 at 2107.682 kb on - strand, within CA265_RS08450 at 2107.682 kb on - strand, within CA265_RS08450 at 2107.722 kb on + strand, within CA265_RS08450 at 2107.758 kb on + strand, within CA265_RS08450 at 2107.765 kb on - strand, within CA265_RS08450 at 2107.787 kb on - strand at 2107.828 kb on + strand, within CA265_RS08450 at 2107.828 kb on + strand, within CA265_RS08450 at 2107.829 kb on - strand, within CA265_RS08450 at 2107.864 kb on - strand, within CA265_RS08450 at 2107.872 kb on + strand, within CA265_RS08450 at 2107.872 kb on + strand, within CA265_RS08450 at 2107.937 kb on + strand at 2107.982 kb on + strand, within CA265_RS08450 at 2107.982 kb on + strand, within CA265_RS08450 at 2107.983 kb on - strand, within CA265_RS08450 at 2108.016 kb on - strand, within CA265_RS08450 at 2108.063 kb on - strand, within CA265_RS08450 at 2108.093 kb on - strand, within CA265_RS08450 at 2108.111 kb on + strand, within CA265_RS08450 at 2108.144 kb on + strand, within CA265_RS08450 at 2108.228 kb on - strand, within CA265_RS08450 at 2108.247 kb on + strand, within CA265_RS08450 at 2108.303 kb on + strand at 2108.401 kb on + strand at 2108.410 kb on + strand at 2108.548 kb on - strand at 2108.548 kb on - strand at 2108.552 kb on + strand at 2108.553 kb on - strand at 2108.570 kb on + strand at 2108.602 kb on + strand at 2108.603 kb on - strand at 2108.627 kb on - strand at 2108.708 kb on + strand at 2108.767 kb on - strand, within CA265_RS08455 at 2108.767 kb on - strand, within CA265_RS08455 at 2108.797 kb on + strand, within CA265_RS08455 at 2108.798 kb on - strand, within CA265_RS08455 at 2108.835 kb on + strand, within CA265_RS08455 at 2108.850 kb on + strand, within CA265_RS08455 at 2108.851 kb on - strand, within CA265_RS08455 at 2108.875 kb on + strand, within CA265_RS08455 at 2108.876 kb on - strand, within CA265_RS08455 at 2108.926 kb on + strand, within CA265_RS08455 at 2108.926 kb on + strand, within CA265_RS08455 at 2108.927 kb on - strand, within CA265_RS08455 at 2108.961 kb on - strand, within CA265_RS08455 at 2108.980 kb on - strand, within CA265_RS08455 at 2108.980 kb on - strand, within CA265_RS08455 at 2108.984 kb on + strand, within CA265_RS08455 at 2109.017 kb on + strand, within CA265_RS08455 at 2109.017 kb on + strand, within CA265_RS08455 at 2109.155 kb on - strand, within CA265_RS08455 at 2109.155 kb on - strand, within CA265_RS08455 at 2109.163 kb on + strand, within CA265_RS08455 at 2109.205 kb on - strand, within CA265_RS08455 at 2109.232 kb on + strand, within CA265_RS08455 at 2109.306 kb on + strand, within CA265_RS08455 at 2109.306 kb on + strand, within CA265_RS08455 at 2109.307 kb on - strand, within CA265_RS08455 at 2109.337 kb on + strand, within CA265_RS08455 at 2109.337 kb on + strand, within CA265_RS08455 at 2109.383 kb on + strand, within CA265_RS08455 at 2109.443 kb on + strand, within CA265_RS08455 at 2109.444 kb on - strand, within CA265_RS08455 at 2109.497 kb on - strand, within CA265_RS08455 at 2109.513 kb on - strand, within CA265_RS08455 at 2109.513 kb on - strand, within CA265_RS08455 at 2109.518 kb on + strand, within CA265_RS08455 at 2109.519 kb on - strand, within CA265_RS08455 at 2109.549 kb on - strand, within CA265_RS08455 at 2109.555 kb on - strand, within CA265_RS08455 at 2109.563 kb on - strand, within CA265_RS08455
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 2,104,456 - CA265_RS08440 0.74 -0.3 2,104,459 + CA265_RS08440 0.74 +0.7 2,104,459 + CA265_RS08440 0.74 +1.0 2,104,558 + CA265_RS08440 0.80 +1.5 2,104,559 - CA265_RS08440 0.80 -0.0 2,104,628 + CA265_RS08440 0.84 -0.8 2,104,628 + CA265_RS08440 0.84 -0.9 2,104,632 + CA265_RS08440 0.84 -0.4 2,104,658 + +1.7 2,104,780 + -0.2 2,104,804 + +1.1 2,104,844 + -0.1 2,104,897 - -0.7 2,104,897 - +0.1 2,104,899 - +0.1 2,104,958 + +0.7 2,104,958 + +0.2 2,104,983 + +0.4 2,104,984 - -0.6 2,105,038 + CA265_RS08445 0.25 -1.6 2,105,298 + +0.1 2,105,308 + +0.3 2,105,308 + +0.8 2,105,309 - +0.3 2,105,384 + -0.4 2,105,385 - -0.0 2,105,385 - -0.4 2,105,430 - -0.2 2,105,450 + +0.5 2,105,451 - +0.2 2,105,468 + -0.4 2,105,490 - +0.8 2,105,590 - +0.6 2,105,638 + -1.2 2,105,738 - -0.7 2,105,803 + CA265_RS08450 0.12 +0.6 2,105,803 + CA265_RS08450 0.12 -0.6 2,105,803 + CA265_RS08450 0.12 +0.0 2,105,803 + CA265_RS08450 0.12 -0.7 2,105,804 - CA265_RS08450 0.12 +0.6 2,105,907 + CA265_RS08450 0.15 +0.6 2,105,908 - CA265_RS08450 0.15 -0.3 2,105,908 - CA265_RS08450 0.15 -0.1 2,105,973 + CA265_RS08450 0.17 +0.5 2,105,974 - CA265_RS08450 0.17 -2.1 2,105,978 - CA265_RS08450 0.17 +0.0 2,105,978 - CA265_RS08450 0.17 +0.6 2,105,978 - CA265_RS08450 0.17 -0.7 2,106,176 + CA265_RS08450 0.24 +0.4 2,106,459 + CA265_RS08450 0.33 +1.2 2,106,475 - CA265_RS08450 0.33 -0.3 2,106,520 - CA265_RS08450 0.34 -0.1 2,106,572 - CA265_RS08450 0.36 -0.4 2,106,577 + CA265_RS08450 0.36 -0.4 2,106,640 + CA265_RS08450 0.38 +1.4 2,106,704 + CA265_RS08450 0.40 -0.4 2,106,786 - CA265_RS08450 0.43 -0.1 2,106,799 - CA265_RS08450 0.43 +0.7 2,106,925 + CA265_RS08450 0.47 +0.4 2,106,926 - CA265_RS08450 0.47 -0.3 2,106,954 + CA265_RS08450 0.48 +0.2 2,106,955 - CA265_RS08450 0.48 +0.0 2,107,025 + CA265_RS08450 0.50 +0.3 2,107,054 - CA265_RS08450 0.51 +0.4 2,107,104 + CA265_RS08450 0.53 -0.5 2,107,138 - CA265_RS08450 0.54 -0.7 2,107,143 + CA265_RS08450 0.54 +0.6 2,107,164 + CA265_RS08450 0.55 +1.6 2,107,283 - CA265_RS08450 0.59 -0.0 2,107,347 - CA265_RS08450 0.61 -0.4 2,107,347 - CA265_RS08450 0.61 +3.2 2,107,368 + CA265_RS08450 0.61 +0.1 2,107,369 - +1.2 2,107,455 + CA265_RS08450 0.64 +1.8 2,107,549 + CA265_RS08450 0.67 -0.8 2,107,550 - CA265_RS08450 0.67 -0.4 2,107,566 + CA265_RS08450 0.68 +1.0 2,107,566 + CA265_RS08450 0.68 -0.6 2,107,584 + CA265_RS08450 0.68 -0.1 2,107,585 - CA265_RS08450 0.68 +0.7 2,107,589 + CA265_RS08450 0.68 +0.4 2,107,660 - CA265_RS08450 0.70 -0.5 2,107,681 + CA265_RS08450 0.71 +1.7 2,107,682 - CA265_RS08450 0.71 +0.4 2,107,682 - CA265_RS08450 0.71 +0.2 2,107,682 - CA265_RS08450 0.71 -0.4 2,107,722 + CA265_RS08450 0.72 +1.2 2,107,758 + CA265_RS08450 0.74 +0.4 2,107,765 - CA265_RS08450 0.74 -0.8 2,107,787 - +0.7 2,107,828 + CA265_RS08450 0.76 +0.5 2,107,828 + CA265_RS08450 0.76 +0.3 2,107,829 - CA265_RS08450 0.76 +0.1 2,107,864 - CA265_RS08450 0.77 +0.5 2,107,872 + CA265_RS08450 0.77 +0.3 2,107,872 + CA265_RS08450 0.77 +0.6 2,107,937 + +1.2 2,107,982 + CA265_RS08450 0.81 +1.9 2,107,982 + CA265_RS08450 0.81 -1.0 2,107,983 - CA265_RS08450 0.81 +0.4 2,108,016 - CA265_RS08450 0.82 -0.1 2,108,063 - CA265_RS08450 0.83 +0.3 2,108,093 - CA265_RS08450 0.84 -0.4 2,108,111 + CA265_RS08450 0.85 +1.7 2,108,144 + CA265_RS08450 0.86 +0.5 2,108,228 - CA265_RS08450 0.88 -2.7 2,108,247 + CA265_RS08450 0.89 -2.8 2,108,303 + +0.5 2,108,401 + +2.1 2,108,410 + +0.9 2,108,548 - -0.5 2,108,548 - -0.3 2,108,552 + -0.1 2,108,553 - -0.0 2,108,570 + -0.7 2,108,602 + +0.1 2,108,603 - +0.4 2,108,627 - -0.4 2,108,708 + +0.4 2,108,767 - CA265_RS08455 0.12 +0.5 2,108,767 - CA265_RS08455 0.12 -0.6 2,108,797 + CA265_RS08455 0.14 +0.5 2,108,798 - CA265_RS08455 0.15 -0.2 2,108,835 + CA265_RS08455 0.17 +0.6 2,108,850 + CA265_RS08455 0.18 +0.1 2,108,851 - CA265_RS08455 0.18 +0.1 2,108,875 + CA265_RS08455 0.20 -0.0 2,108,876 - CA265_RS08455 0.20 +1.2 2,108,926 + CA265_RS08455 0.24 -0.0 2,108,926 + CA265_RS08455 0.24 -0.4 2,108,927 - CA265_RS08455 0.24 +0.7 2,108,961 - CA265_RS08455 0.26 +0.5 2,108,980 - CA265_RS08455 0.28 -0.1 2,108,980 - CA265_RS08455 0.28 -1.3 2,108,984 + CA265_RS08455 0.28 +0.1 2,109,017 + CA265_RS08455 0.30 +0.4 2,109,017 + CA265_RS08455 0.30 +0.8 2,109,155 - CA265_RS08455 0.40 -0.5 2,109,155 - CA265_RS08455 0.40 +1.4 2,109,163 + CA265_RS08455 0.41 -0.8 2,109,205 - CA265_RS08455 0.44 -0.3 2,109,232 + CA265_RS08455 0.46 +0.2 2,109,306 + CA265_RS08455 0.51 +0.2 2,109,306 + CA265_RS08455 0.51 -0.1 2,109,307 - CA265_RS08455 0.51 -0.2 2,109,337 + CA265_RS08455 0.53 +0.3 2,109,337 + CA265_RS08455 0.53 +0.0 2,109,383 + CA265_RS08455 0.56 +1.1 2,109,443 + CA265_RS08455 0.61 +0.1 2,109,444 - CA265_RS08455 0.61 -0.8 2,109,497 - CA265_RS08455 0.64 +0.3 2,109,513 - CA265_RS08455 0.66 -0.1 2,109,513 - CA265_RS08455 0.66 +0.3 2,109,518 + CA265_RS08455 0.66 -0.2 2,109,519 - CA265_RS08455 0.66 -0.7 2,109,549 - CA265_RS08455 0.68 -0.7 2,109,555 - CA265_RS08455 0.69 -1.2 2,109,563 - CA265_RS08455 0.69 +0.4
Or see this region's nucleotide sequence