Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS07915 and CA265_RS07920 overlap by 4 nucleotides CA265_RS07920 and CA265_RS07925 are separated by 0 nucleotides CA265_RS07925 and CA265_RS07930 are separated by 6 nucleotides
CA265_RS07915: CA265_RS07915 - lipopolysaccharide biosynthesis protein, at 1,975,281 to 1,976,723
_RS07915
CA265_RS07920: CA265_RS07920 - aminotransferase DegT, at 1,976,720 to 1,977,877
_RS07920
CA265_RS07925: CA265_RS07925 - hypothetical protein, at 1,977,878 to 1,978,762
_RS07925
CA265_RS07930: CA265_RS07930 - group 1 glycosyl transferase, at 1,978,769 to 1,979,863
_RS07930
Position (kb)
1976
1977
1978 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1975.970 kb on + strand, within CA265_RS07915 at 1975.973 kb on + strand, within CA265_RS07915 at 1975.974 kb on - strand, within CA265_RS07915 at 1975.974 kb on - strand, within CA265_RS07915 at 1975.975 kb on + strand, within CA265_RS07915 at 1975.976 kb on - strand, within CA265_RS07915 at 1976.024 kb on + strand, within CA265_RS07915 at 1976.025 kb on - strand, within CA265_RS07915 at 1976.058 kb on - strand, within CA265_RS07915 at 1976.066 kb on + strand, within CA265_RS07915 at 1976.070 kb on - strand, within CA265_RS07915 at 1976.141 kb on - strand, within CA265_RS07915 at 1976.141 kb on - strand, within CA265_RS07915 at 1976.141 kb on - strand, within CA265_RS07915 at 1976.152 kb on + strand, within CA265_RS07915 at 1976.207 kb on - strand, within CA265_RS07915 at 1976.245 kb on + strand, within CA265_RS07915 at 1976.251 kb on - strand, within CA265_RS07915 at 1976.264 kb on - strand, within CA265_RS07915 at 1976.281 kb on + strand, within CA265_RS07915 at 1976.281 kb on + strand, within CA265_RS07915 at 1976.282 kb on - strand, within CA265_RS07915 at 1976.299 kb on - strand, within CA265_RS07915 at 1976.299 kb on - strand, within CA265_RS07915 at 1976.299 kb on - strand, within CA265_RS07915 at 1976.330 kb on + strand, within CA265_RS07915 at 1976.396 kb on - strand, within CA265_RS07915 at 1976.425 kb on - strand, within CA265_RS07915 at 1976.473 kb on - strand, within CA265_RS07915 at 1976.475 kb on - strand, within CA265_RS07915 at 1976.596 kb on - strand at 1976.610 kb on - strand at 1976.629 kb on + strand at 1976.675 kb on + strand at 1976.676 kb on - strand at 1976.734 kb on + strand at 1976.775 kb on + strand at 1976.775 kb on + strand at 1976.776 kb on - strand at 1976.852 kb on + strand, within CA265_RS07920 at 1976.880 kb on + strand, within CA265_RS07920 at 1976.922 kb on + strand, within CA265_RS07920 at 1976.922 kb on + strand, within CA265_RS07920 at 1976.922 kb on + strand, within CA265_RS07920 at 1976.922 kb on + strand, within CA265_RS07920 at 1976.977 kb on + strand, within CA265_RS07920 at 1976.977 kb on + strand, within CA265_RS07920 at 1976.977 kb on + strand, within CA265_RS07920 at 1976.981 kb on - strand, within CA265_RS07920 at 1976.981 kb on - strand, within CA265_RS07920 at 1977.019 kb on - strand, within CA265_RS07920 at 1977.037 kb on + strand, within CA265_RS07920 at 1977.094 kb on + strand, within CA265_RS07920 at 1977.095 kb on - strand, within CA265_RS07920 at 1977.166 kb on - strand, within CA265_RS07920 at 1977.170 kb on + strand, within CA265_RS07920 at 1977.171 kb on - strand, within CA265_RS07920 at 1977.200 kb on + strand, within CA265_RS07920 at 1977.201 kb on - strand, within CA265_RS07920 at 1977.201 kb on - strand, within CA265_RS07920 at 1977.203 kb on - strand, within CA265_RS07920 at 1977.215 kb on - strand, within CA265_RS07920 at 1977.234 kb on + strand, within CA265_RS07920 at 1977.235 kb on - strand, within CA265_RS07920 at 1977.235 kb on - strand, within CA265_RS07920 at 1977.357 kb on + strand, within CA265_RS07920 at 1977.361 kb on + strand, within CA265_RS07920 at 1977.436 kb on + strand, within CA265_RS07920 at 1977.468 kb on + strand, within CA265_RS07920 at 1977.469 kb on - strand, within CA265_RS07920 at 1977.469 kb on - strand, within CA265_RS07920 at 1977.501 kb on + strand, within CA265_RS07920 at 1977.502 kb on - strand, within CA265_RS07920 at 1977.552 kb on - strand, within CA265_RS07920 at 1977.555 kb on - strand, within CA265_RS07920 at 1977.584 kb on - strand, within CA265_RS07920 at 1977.623 kb on + strand, within CA265_RS07920 at 1977.682 kb on + strand, within CA265_RS07920 at 1977.683 kb on - strand, within CA265_RS07920 at 1977.689 kb on - strand, within CA265_RS07920 at 1977.701 kb on - strand, within CA265_RS07920 at 1977.776 kb on - strand at 1977.814 kb on + strand at 1977.828 kb on - strand at 1977.852 kb on + strand at 1977.853 kb on - strand at 1977.853 kb on - strand at 1977.908 kb on - strand at 1977.915 kb on + strand at 1977.916 kb on - strand at 1977.923 kb on - strand at 1977.941 kb on + strand at 1977.944 kb on - strand at 1978.037 kb on - strand, within CA265_RS07925 at 1978.037 kb on - strand, within CA265_RS07925 at 1978.067 kb on + strand, within CA265_RS07925 at 1978.068 kb on - strand, within CA265_RS07925 at 1978.079 kb on + strand, within CA265_RS07925 at 1978.112 kb on - strand, within CA265_RS07925 at 1978.112 kb on - strand, within CA265_RS07925 at 1978.212 kb on - strand, within CA265_RS07925 at 1978.257 kb on - strand, within CA265_RS07925 at 1978.274 kb on + strand, within CA265_RS07925 at 1978.300 kb on + strand, within CA265_RS07925 at 1978.343 kb on + strand, within CA265_RS07925 at 1978.345 kb on + strand, within CA265_RS07925 at 1978.515 kb on + strand, within CA265_RS07925 at 1978.523 kb on - strand, within CA265_RS07925 at 1978.643 kb on + strand, within CA265_RS07925 at 1978.644 kb on - strand, within CA265_RS07925 at 1978.674 kb on - strand at 1978.679 kb on + strand at 1978.691 kb on + strand at 1978.691 kb on + strand at 1978.745 kb on - strand at 1978.782 kb on + strand at 1978.843 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 1,975,970 + CA265_RS07915 0.48 -0.8 1,975,973 + CA265_RS07915 0.48 +0.1 1,975,974 - CA265_RS07915 0.48 -0.4 1,975,974 - CA265_RS07915 0.48 -0.4 1,975,975 + CA265_RS07915 0.48 -3.6 1,975,976 - CA265_RS07915 0.48 -1.4 1,976,024 + CA265_RS07915 0.51 -1.7 1,976,025 - CA265_RS07915 0.52 +0.5 1,976,058 - CA265_RS07915 0.54 -0.9 1,976,066 + CA265_RS07915 0.54 -2.5 1,976,070 - CA265_RS07915 0.55 -1.1 1,976,141 - CA265_RS07915 0.60 -1.2 1,976,141 - CA265_RS07915 0.60 +0.4 1,976,141 - CA265_RS07915 0.60 -0.4 1,976,152 + CA265_RS07915 0.60 -2.4 1,976,207 - CA265_RS07915 0.64 -0.7 1,976,245 + CA265_RS07915 0.67 -3.5 1,976,251 - CA265_RS07915 0.67 +0.7 1,976,264 - CA265_RS07915 0.68 -0.5 1,976,281 + CA265_RS07915 0.69 -1.2 1,976,281 + CA265_RS07915 0.69 -1.1 1,976,282 - CA265_RS07915 0.69 -1.4 1,976,299 - CA265_RS07915 0.71 +0.1 1,976,299 - CA265_RS07915 0.71 +0.7 1,976,299 - CA265_RS07915 0.71 -0.7 1,976,330 + CA265_RS07915 0.73 -0.4 1,976,396 - CA265_RS07915 0.77 +0.9 1,976,425 - CA265_RS07915 0.79 +0.8 1,976,473 - CA265_RS07915 0.83 -0.8 1,976,475 - CA265_RS07915 0.83 -0.4 1,976,596 - -0.8 1,976,610 - +0.5 1,976,629 + -1.4 1,976,675 + -0.9 1,976,676 - +0.1 1,976,734 + -0.6 1,976,775 + -1.7 1,976,775 + -0.5 1,976,776 - +0.8 1,976,852 + CA265_RS07920 0.11 -0.6 1,976,880 + CA265_RS07920 0.14 -1.1 1,976,922 + CA265_RS07920 0.17 -1.5 1,976,922 + CA265_RS07920 0.17 -1.0 1,976,922 + CA265_RS07920 0.17 -0.1 1,976,922 + CA265_RS07920 0.17 -1.1 1,976,977 + CA265_RS07920 0.22 -0.5 1,976,977 + CA265_RS07920 0.22 -1.5 1,976,977 + CA265_RS07920 0.22 +0.5 1,976,981 - CA265_RS07920 0.23 -0.4 1,976,981 - CA265_RS07920 0.23 -0.6 1,977,019 - CA265_RS07920 0.26 +1.3 1,977,037 + CA265_RS07920 0.27 -3.2 1,977,094 + CA265_RS07920 0.32 -1.6 1,977,095 - CA265_RS07920 0.32 -0.2 1,977,166 - CA265_RS07920 0.39 -1.4 1,977,170 + CA265_RS07920 0.39 -0.6 1,977,171 - CA265_RS07920 0.39 -0.0 1,977,200 + CA265_RS07920 0.41 -1.4 1,977,201 - CA265_RS07920 0.42 -0.4 1,977,201 - CA265_RS07920 0.42 -0.7 1,977,203 - CA265_RS07920 0.42 -0.3 1,977,215 - CA265_RS07920 0.43 +0.0 1,977,234 + CA265_RS07920 0.44 -0.1 1,977,235 - CA265_RS07920 0.44 +0.6 1,977,235 - CA265_RS07920 0.44 -0.6 1,977,357 + CA265_RS07920 0.55 -1.8 1,977,361 + CA265_RS07920 0.55 -2.0 1,977,436 + CA265_RS07920 0.62 -2.5 1,977,468 + CA265_RS07920 0.65 -1.3 1,977,469 - CA265_RS07920 0.65 +1.8 1,977,469 - CA265_RS07920 0.65 +0.5 1,977,501 + CA265_RS07920 0.67 -2.0 1,977,502 - CA265_RS07920 0.68 -0.3 1,977,552 - CA265_RS07920 0.72 +1.0 1,977,555 - CA265_RS07920 0.72 +0.4 1,977,584 - CA265_RS07920 0.75 +0.4 1,977,623 + CA265_RS07920 0.78 -1.4 1,977,682 + CA265_RS07920 0.83 -1.8 1,977,683 - CA265_RS07920 0.83 -0.7 1,977,689 - CA265_RS07920 0.84 -1.9 1,977,701 - CA265_RS07920 0.85 -0.7 1,977,776 - +0.6 1,977,814 + -1.4 1,977,828 - +0.7 1,977,852 + +0.3 1,977,853 - -1.1 1,977,853 - -0.2 1,977,908 - -2.1 1,977,915 + -0.2 1,977,916 - +0.5 1,977,923 - -0.0 1,977,941 + -2.0 1,977,944 - -0.6 1,978,037 - CA265_RS07925 0.18 +0.5 1,978,037 - CA265_RS07925 0.18 +0.5 1,978,067 + CA265_RS07925 0.21 -1.2 1,978,068 - CA265_RS07925 0.21 +0.3 1,978,079 + CA265_RS07925 0.23 +0.4 1,978,112 - CA265_RS07925 0.26 -0.1 1,978,112 - CA265_RS07925 0.26 +0.3 1,978,212 - CA265_RS07925 0.38 +2.0 1,978,257 - CA265_RS07925 0.43 -1.3 1,978,274 + CA265_RS07925 0.45 -0.9 1,978,300 + CA265_RS07925 0.48 -1.9 1,978,343 + CA265_RS07925 0.53 -0.2 1,978,345 + CA265_RS07925 0.53 -1.0 1,978,515 + CA265_RS07925 0.72 -0.9 1,978,523 - CA265_RS07925 0.73 +1.2 1,978,643 + CA265_RS07925 0.86 -0.9 1,978,644 - CA265_RS07925 0.87 +0.4 1,978,674 - -0.4 1,978,679 + -1.5 1,978,691 + +0.4 1,978,691 + -1.0 1,978,745 - +0.3 1,978,782 + -2.0 1,978,843 + -1.7
Or see this region's nucleotide sequence