Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS07345 and CA265_RS07350 are separated by 237 nucleotides CA265_RS07350 and CA265_RS07355 are separated by 25 nucleotides
CA265_RS07345: CA265_RS07345 - HAD family hydrolase, at 1,851,120 to 1,851,791
_RS07345
CA265_RS07350: CA265_RS07350 - alcohol dehydrogenase, at 1,852,029 to 1,853,111
_RS07350
CA265_RS07355: CA265_RS07355 - MFS transporter, at 1,853,137 to 1,854,336
_RS07355
Position (kb)
1852
1853
1854 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1851.060 kb on + strand at 1851.166 kb on - strand at 1851.168 kb on + strand at 1851.235 kb on - strand, within CA265_RS07345 at 1851.344 kb on + strand, within CA265_RS07345 at 1851.344 kb on + strand, within CA265_RS07345 at 1851.392 kb on + strand, within CA265_RS07345 at 1851.467 kb on + strand, within CA265_RS07345 at 1851.467 kb on + strand, within CA265_RS07345 at 1851.471 kb on + strand, within CA265_RS07345 at 1851.584 kb on - strand, within CA265_RS07345 at 1851.587 kb on + strand, within CA265_RS07345 at 1851.634 kb on - strand, within CA265_RS07345 at 1851.696 kb on - strand, within CA265_RS07345 at 1851.718 kb on - strand, within CA265_RS07345 at 1851.784 kb on - strand at 1851.866 kb on + strand at 1852.094 kb on + strand at 1852.137 kb on + strand at 1852.137 kb on + strand at 1852.139 kb on + strand, within CA265_RS07350 at 1852.142 kb on + strand, within CA265_RS07350 at 1852.167 kb on - strand, within CA265_RS07350 at 1852.232 kb on - strand, within CA265_RS07350 at 1852.267 kb on - strand, within CA265_RS07350 at 1852.270 kb on + strand, within CA265_RS07350 at 1852.270 kb on + strand, within CA265_RS07350 at 1852.271 kb on - strand, within CA265_RS07350 at 1852.383 kb on + strand, within CA265_RS07350 at 1852.406 kb on + strand, within CA265_RS07350 at 1852.465 kb on + strand, within CA265_RS07350 at 1852.465 kb on + strand, within CA265_RS07350 at 1852.466 kb on - strand, within CA265_RS07350 at 1852.493 kb on + strand, within CA265_RS07350 at 1852.494 kb on - strand, within CA265_RS07350 at 1852.511 kb on + strand, within CA265_RS07350 at 1852.576 kb on + strand, within CA265_RS07350 at 1852.576 kb on + strand, within CA265_RS07350 at 1852.609 kb on + strand, within CA265_RS07350 at 1852.663 kb on + strand, within CA265_RS07350 at 1852.721 kb on - strand, within CA265_RS07350 at 1852.749 kb on - strand, within CA265_RS07350 at 1852.767 kb on - strand, within CA265_RS07350 at 1852.812 kb on - strand, within CA265_RS07350 at 1852.812 kb on - strand, within CA265_RS07350 at 1852.812 kb on - strand, within CA265_RS07350 at 1852.902 kb on - strand, within CA265_RS07350 at 1852.924 kb on - strand, within CA265_RS07350 at 1853.044 kb on - strand at 1853.057 kb on + strand at 1853.057 kb on + strand at 1853.057 kb on + strand at 1853.058 kb on - strand at 1853.094 kb on - strand at 1853.099 kb on + strand at 1853.100 kb on - strand at 1853.230 kb on - strand at 1853.230 kb on - strand at 1853.231 kb on + strand at 1853.231 kb on + strand at 1853.238 kb on + strand at 1853.354 kb on - strand, within CA265_RS07355 at 1853.374 kb on - strand, within CA265_RS07355 at 1853.478 kb on + strand, within CA265_RS07355 at 1853.523 kb on + strand, within CA265_RS07355 at 1853.524 kb on - strand, within CA265_RS07355 at 1853.524 kb on - strand, within CA265_RS07355 at 1853.558 kb on - strand, within CA265_RS07355 at 1853.569 kb on - strand, within CA265_RS07355 at 1853.648 kb on + strand, within CA265_RS07355 at 1853.655 kb on - strand, within CA265_RS07355 at 1853.659 kb on - strand, within CA265_RS07355 at 1853.822 kb on + strand, within CA265_RS07355 at 1853.865 kb on + strand, within CA265_RS07355 at 1853.895 kb on - strand, within CA265_RS07355 at 1853.946 kb on + strand, within CA265_RS07355
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 1,851,060 + +0.7 1,851,166 - +0.4 1,851,168 + +0.4 1,851,235 - CA265_RS07345 0.17 -0.7 1,851,344 + CA265_RS07345 0.33 +0.4 1,851,344 + CA265_RS07345 0.33 -1.0 1,851,392 + CA265_RS07345 0.40 -1.1 1,851,467 + CA265_RS07345 0.52 +0.4 1,851,467 + CA265_RS07345 0.52 +0.7 1,851,471 + CA265_RS07345 0.52 -0.3 1,851,584 - CA265_RS07345 0.69 -0.4 1,851,587 + CA265_RS07345 0.69 -0.1 1,851,634 - CA265_RS07345 0.76 +0.1 1,851,696 - CA265_RS07345 0.86 +0.7 1,851,718 - CA265_RS07345 0.89 -0.3 1,851,784 - +1.1 1,851,866 + -0.8 1,852,094 + -1.4 1,852,137 + -0.2 1,852,137 + -0.8 1,852,139 + CA265_RS07350 0.10 -0.4 1,852,142 + CA265_RS07350 0.10 +0.4 1,852,167 - CA265_RS07350 0.13 +0.2 1,852,232 - CA265_RS07350 0.19 +0.1 1,852,267 - CA265_RS07350 0.22 -0.6 1,852,270 + CA265_RS07350 0.22 +0.4 1,852,270 + CA265_RS07350 0.22 -0.5 1,852,271 - CA265_RS07350 0.22 -0.6 1,852,383 + CA265_RS07350 0.33 +0.5 1,852,406 + CA265_RS07350 0.35 +0.7 1,852,465 + CA265_RS07350 0.40 -0.3 1,852,465 + CA265_RS07350 0.40 -1.7 1,852,466 - CA265_RS07350 0.40 +0.3 1,852,493 + CA265_RS07350 0.43 -0.1 1,852,494 - CA265_RS07350 0.43 -1.4 1,852,511 + CA265_RS07350 0.45 -0.9 1,852,576 + CA265_RS07350 0.51 -0.8 1,852,576 + CA265_RS07350 0.51 -2.1 1,852,609 + CA265_RS07350 0.54 +0.5 1,852,663 + CA265_RS07350 0.59 +0.6 1,852,721 - CA265_RS07350 0.64 +0.1 1,852,749 - CA265_RS07350 0.66 -1.1 1,852,767 - CA265_RS07350 0.68 -0.2 1,852,812 - CA265_RS07350 0.72 -0.1 1,852,812 - CA265_RS07350 0.72 -1.2 1,852,812 - CA265_RS07350 0.72 -0.3 1,852,902 - CA265_RS07350 0.81 -0.5 1,852,924 - CA265_RS07350 0.83 -0.6 1,853,044 - -1.1 1,853,057 + -0.9 1,853,057 + -1.3 1,853,057 + +0.0 1,853,058 - -0.5 1,853,094 - -0.2 1,853,099 + +1.2 1,853,100 - -0.7 1,853,230 - +0.7 1,853,230 - +1.3 1,853,231 + -0.1 1,853,231 + +0.2 1,853,238 + -0.1 1,853,354 - CA265_RS07355 0.18 -0.0 1,853,374 - CA265_RS07355 0.20 -0.8 1,853,478 + CA265_RS07355 0.28 +1.9 1,853,523 + CA265_RS07355 0.32 +0.5 1,853,524 - CA265_RS07355 0.32 +2.2 1,853,524 - CA265_RS07355 0.32 -0.1 1,853,558 - CA265_RS07355 0.35 -0.1 1,853,569 - CA265_RS07355 0.36 +0.2 1,853,648 + CA265_RS07355 0.43 +2.7 1,853,655 - CA265_RS07355 0.43 -0.4 1,853,659 - CA265_RS07355 0.43 +0.4 1,853,822 + CA265_RS07355 0.57 +0.8 1,853,865 + CA265_RS07355 0.61 +0.7 1,853,895 - CA265_RS07355 0.63 -1.4 1,853,946 + CA265_RS07355 0.67 -0.4
Or see this region's nucleotide sequence