Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS04880 and CA265_RS04885 are separated by 67 nucleotides CA265_RS04885 and CA265_RS04890 are separated by 203 nucleotides CA265_RS04890 and CA265_RS04895 are separated by 237 nucleotides
CA265_RS04880: CA265_RS04880 - tRNA-Leu, at 1,164,954 to 1,165,037
_RS04880
CA265_RS04885: CA265_RS04885 - tRNA-Gly, at 1,165,105 to 1,165,180
_RS04885
CA265_RS04890: CA265_RS04890 - hypothetical protein, at 1,165,384 to 1,165,694
_RS04890
CA265_RS04895: CA265_RS04895 - hypothetical protein, at 1,165,932 to 1,167,032
_RS04895
Position (kb)
1165
1166 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1164.420 kb on - strand at 1164.519 kb on + strand at 1164.520 kb on - strand at 1164.520 kb on - strand at 1164.520 kb on - strand at 1164.594 kb on - strand at 1164.611 kb on - strand at 1164.699 kb on + strand at 1164.738 kb on - strand at 1164.790 kb on - strand at 1165.160 kb on + strand, within CA265_RS04885 at 1165.198 kb on - strand at 1165.363 kb on + strand at 1165.364 kb on - strand at 1165.364 kb on - strand at 1165.366 kb on - strand at 1165.378 kb on - strand at 1165.409 kb on - strand at 1165.474 kb on + strand, within CA265_RS04890 at 1165.474 kb on + strand, within CA265_RS04890 at 1165.475 kb on - strand, within CA265_RS04890 at 1165.530 kb on - strand, within CA265_RS04890 at 1165.628 kb on - strand, within CA265_RS04890 at 1165.632 kb on + strand, within CA265_RS04890 at 1165.637 kb on + strand, within CA265_RS04890 at 1165.649 kb on - strand, within CA265_RS04890 at 1165.679 kb on + strand at 1165.688 kb on + strand at 1165.689 kb on - strand at 1165.690 kb on + strand at 1165.691 kb on - strand at 1165.693 kb on - strand at 1165.693 kb on - strand at 1165.712 kb on + strand at 1165.800 kb on + strand at 1165.820 kb on + strand at 1165.900 kb on - strand at 1165.911 kb on + strand at 1165.932 kb on - strand at 1165.979 kb on - strand at 1166.007 kb on + strand at 1166.035 kb on + strand at 1166.156 kb on + strand, within CA265_RS04895 at 1166.156 kb on + strand, within CA265_RS04895 at 1166.260 kb on + strand, within CA265_RS04895 at 1166.271 kb on + strand, within CA265_RS04895 at 1166.271 kb on + strand, within CA265_RS04895 at 1166.272 kb on - strand, within CA265_RS04895 at 1166.310 kb on + strand, within CA265_RS04895 at 1166.310 kb on + strand, within CA265_RS04895 at 1166.364 kb on - strand, within CA265_RS04895 at 1166.603 kb on + strand, within CA265_RS04895 at 1166.608 kb on + strand, within CA265_RS04895 at 1166.651 kb on + strand, within CA265_RS04895
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 1,164,420 - -0.6 1,164,519 + -0.2 1,164,520 - -0.2 1,164,520 - -0.5 1,164,520 - -0.3 1,164,594 - -0.9 1,164,611 - +1.8 1,164,699 + -1.0 1,164,738 - +0.1 1,164,790 - +0.7 1,165,160 + CA265_RS04885 0.72 +0.1 1,165,198 - -0.9 1,165,363 + +1.7 1,165,364 - +0.1 1,165,364 - -0.1 1,165,366 - +2.0 1,165,378 - +0.1 1,165,409 - -0.5 1,165,474 + CA265_RS04890 0.29 -1.2 1,165,474 + CA265_RS04890 0.29 -1.0 1,165,475 - CA265_RS04890 0.29 -0.6 1,165,530 - CA265_RS04890 0.47 +0.2 1,165,628 - CA265_RS04890 0.78 +0.1 1,165,632 + CA265_RS04890 0.80 -1.2 1,165,637 + CA265_RS04890 0.81 -0.3 1,165,649 - CA265_RS04890 0.85 +0.1 1,165,679 + +1.3 1,165,688 + -0.9 1,165,689 - -1.7 1,165,690 + -0.0 1,165,691 - -0.2 1,165,693 - +0.7 1,165,693 - -1.4 1,165,712 + +0.1 1,165,800 + -0.0 1,165,820 + +0.8 1,165,900 - +0.7 1,165,911 + -0.8 1,165,932 - -1.8 1,165,979 - -0.1 1,166,007 + +0.0 1,166,035 + +0.1 1,166,156 + CA265_RS04895 0.20 +1.7 1,166,156 + CA265_RS04895 0.20 -0.7 1,166,260 + CA265_RS04895 0.30 -0.3 1,166,271 + CA265_RS04895 0.31 -0.0 1,166,271 + CA265_RS04895 0.31 +0.1 1,166,272 - CA265_RS04895 0.31 +0.3 1,166,310 + CA265_RS04895 0.34 +0.8 1,166,310 + CA265_RS04895 0.34 -0.1 1,166,364 - CA265_RS04895 0.39 -0.5 1,166,603 + CA265_RS04895 0.61 -0.4 1,166,608 + CA265_RS04895 0.61 +0.9 1,166,651 + CA265_RS04895 0.65 -0.1
Or see this region's nucleotide sequence