Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS04370 and CA265_RS04375 are separated by 157 nucleotides CA265_RS04375 and CA265_RS04380 are separated by 324 nucleotides
CA265_RS04370: CA265_RS04370 - DNA protecting protein DprA, at 1,048,718 to 1,049,812
_RS04370
CA265_RS04375: CA265_RS04375 - cystathionine beta-synthase, at 1,049,970 to 1,051,331
_RS04375
CA265_RS04380: CA265_RS04380 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase, at 1,051,656 to 1,052,981
_RS04380
Position (kb)
1049
1050
1051
1052 Strain fitness (log2 ratio)
-2
-1
0
1 at 1048.978 kb on + strand, within CA265_RS04370 at 1049.053 kb on + strand, within CA265_RS04370 at 1049.053 kb on + strand, within CA265_RS04370 at 1049.053 kb on + strand, within CA265_RS04370 at 1049.127 kb on + strand, within CA265_RS04370 at 1049.135 kb on + strand, within CA265_RS04370 at 1049.135 kb on + strand, within CA265_RS04370 at 1049.135 kb on + strand, within CA265_RS04370 at 1049.136 kb on - strand, within CA265_RS04370 at 1049.136 kb on - strand, within CA265_RS04370 at 1049.179 kb on - strand, within CA265_RS04370 at 1049.209 kb on + strand, within CA265_RS04370 at 1049.253 kb on - strand, within CA265_RS04370 at 1049.368 kb on + strand, within CA265_RS04370 at 1049.398 kb on - strand, within CA265_RS04370 at 1049.398 kb on - strand, within CA265_RS04370 at 1049.455 kb on + strand, within CA265_RS04370 at 1049.459 kb on - strand, within CA265_RS04370 at 1049.467 kb on - strand, within CA265_RS04370 at 1049.467 kb on - strand, within CA265_RS04370 at 1049.468 kb on + strand, within CA265_RS04370 at 1049.469 kb on - strand, within CA265_RS04370 at 1049.469 kb on - strand, within CA265_RS04370 at 1049.469 kb on - strand, within CA265_RS04370 at 1049.548 kb on - strand, within CA265_RS04370 at 1049.548 kb on - strand, within CA265_RS04370 at 1049.570 kb on + strand, within CA265_RS04370 at 1049.634 kb on + strand, within CA265_RS04370 at 1049.693 kb on - strand, within CA265_RS04370 at 1049.731 kb on + strand at 1049.774 kb on - strand at 1049.796 kb on + strand at 1049.796 kb on + strand at 1049.797 kb on - strand at 1049.800 kb on + strand at 1049.800 kb on + strand at 1049.800 kb on - strand at 1049.945 kb on + strand at 1050.074 kb on + strand at 1050.075 kb on - strand at 1050.075 kb on - strand at 1050.167 kb on + strand at 1050.169 kb on + strand, within CA265_RS04375 at 1050.439 kb on - strand, within CA265_RS04375 at 1050.626 kb on - strand, within CA265_RS04375 at 1050.780 kb on - strand, within CA265_RS04375 at 1050.820 kb on + strand, within CA265_RS04375 at 1050.968 kb on + strand, within CA265_RS04375 at 1050.969 kb on - strand, within CA265_RS04375 at 1051.041 kb on - strand, within CA265_RS04375 at 1051.150 kb on + strand, within CA265_RS04375 at 1051.205 kb on - strand at 1051.239 kb on - strand at 1051.302 kb on + strand at 1051.303 kb on - strand at 1051.303 kb on - strand at 1051.307 kb on + strand at 1051.329 kb on + strand at 1051.354 kb on + strand at 1051.355 kb on - strand at 1051.393 kb on - strand at 1051.501 kb on - strand at 1051.514 kb on - strand at 1051.514 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 1,048,978 + CA265_RS04370 0.24 -0.1 1,049,053 + CA265_RS04370 0.31 -2.0 1,049,053 + CA265_RS04370 0.31 +0.3 1,049,053 + CA265_RS04370 0.31 -0.3 1,049,127 + CA265_RS04370 0.37 +0.2 1,049,135 + CA265_RS04370 0.38 -0.4 1,049,135 + CA265_RS04370 0.38 +0.2 1,049,135 + CA265_RS04370 0.38 -0.6 1,049,136 - CA265_RS04370 0.38 -0.2 1,049,136 - CA265_RS04370 0.38 +1.0 1,049,179 - CA265_RS04370 0.42 +0.6 1,049,209 + CA265_RS04370 0.45 -0.2 1,049,253 - CA265_RS04370 0.49 -0.6 1,049,368 + CA265_RS04370 0.59 +0.6 1,049,398 - CA265_RS04370 0.62 +1.2 1,049,398 - CA265_RS04370 0.62 +0.3 1,049,455 + CA265_RS04370 0.67 +0.2 1,049,459 - CA265_RS04370 0.68 -0.6 1,049,467 - CA265_RS04370 0.68 -1.2 1,049,467 - CA265_RS04370 0.68 -0.5 1,049,468 + CA265_RS04370 0.68 -1.0 1,049,469 - CA265_RS04370 0.69 +1.1 1,049,469 - CA265_RS04370 0.69 -0.5 1,049,469 - CA265_RS04370 0.69 -0.4 1,049,548 - CA265_RS04370 0.76 -0.8 1,049,548 - CA265_RS04370 0.76 -1.2 1,049,570 + CA265_RS04370 0.78 +1.3 1,049,634 + CA265_RS04370 0.84 -0.1 1,049,693 - CA265_RS04370 0.89 +0.1 1,049,731 + -0.3 1,049,774 - -1.2 1,049,796 + +1.0 1,049,796 + +1.6 1,049,797 - +0.1 1,049,800 + +1.1 1,049,800 + +0.4 1,049,800 - -0.9 1,049,945 + +0.5 1,050,074 + -0.2 1,050,075 - -0.5 1,050,075 - -0.1 1,050,167 + +0.6 1,050,169 + CA265_RS04375 0.15 +0.1 1,050,439 - CA265_RS04375 0.34 -1.5 1,050,626 - CA265_RS04375 0.48 -1.4 1,050,780 - CA265_RS04375 0.59 -0.1 1,050,820 + CA265_RS04375 0.62 +1.0 1,050,968 + CA265_RS04375 0.73 -1.1 1,050,969 - CA265_RS04375 0.73 +1.0 1,051,041 - CA265_RS04375 0.79 -0.9 1,051,150 + CA265_RS04375 0.87 -1.2 1,051,205 - +1.6 1,051,239 - -1.4 1,051,302 + -1.3 1,051,303 - -0.4 1,051,303 - -0.4 1,051,307 + -0.4 1,051,329 + +0.3 1,051,354 + -0.6 1,051,355 - +0.4 1,051,393 - +1.0 1,051,501 - +0.6 1,051,514 - -0.7 1,051,514 - -0.4
Or see this region's nucleotide sequence