Experiment: R2A with Vancomycin 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS01810 and CA265_RS01815 are separated by 165 nucleotides CA265_RS01815 and CA265_RS01820 are separated by 152 nucleotides
CA265_RS01810: CA265_RS01810 - asparagine synthase (glutamine-hydrolyzing), at 451,911 to 453,698
_RS01810
CA265_RS01815: CA265_RS01815 - antitermination protein NusG, at 453,864 to 454,367
_RS01815
CA265_RS01820: CA265_RS01820 - hypothetical protein, at 454,520 to 456,925
_RS01820
Position (kb)
453
454
455 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 452.867 kb on + strand, within CA265_RS01810 at 452.879 kb on + strand, within CA265_RS01810 at 452.913 kb on + strand, within CA265_RS01810 at 452.913 kb on + strand, within CA265_RS01810 at 452.921 kb on + strand, within CA265_RS01810 at 452.922 kb on - strand, within CA265_RS01810 at 453.007 kb on + strand, within CA265_RS01810 at 453.008 kb on - strand, within CA265_RS01810 at 453.008 kb on - strand, within CA265_RS01810 at 453.032 kb on - strand, within CA265_RS01810 at 453.032 kb on - strand, within CA265_RS01810 at 453.032 kb on - strand, within CA265_RS01810 at 453.042 kb on - strand, within CA265_RS01810 at 453.117 kb on - strand, within CA265_RS01810 at 453.117 kb on - strand, within CA265_RS01810 at 453.117 kb on - strand, within CA265_RS01810 at 453.118 kb on + strand, within CA265_RS01810 at 453.182 kb on + strand, within CA265_RS01810 at 453.183 kb on - strand, within CA265_RS01810 at 453.194 kb on + strand, within CA265_RS01810 at 453.247 kb on + strand, within CA265_RS01810 at 453.247 kb on + strand, within CA265_RS01810 at 453.248 kb on - strand, within CA265_RS01810 at 453.248 kb on - strand, within CA265_RS01810 at 453.302 kb on + strand, within CA265_RS01810 at 453.326 kb on + strand, within CA265_RS01810 at 453.373 kb on + strand, within CA265_RS01810 at 453.373 kb on + strand, within CA265_RS01810 at 453.411 kb on - strand, within CA265_RS01810 at 453.417 kb on + strand, within CA265_RS01810 at 453.418 kb on - strand, within CA265_RS01810 at 453.449 kb on - strand, within CA265_RS01810 at 453.449 kb on - strand, within CA265_RS01810 at 453.454 kb on + strand, within CA265_RS01810 at 453.469 kb on + strand, within CA265_RS01810 at 453.469 kb on + strand, within CA265_RS01810 at 453.525 kb on - strand at 453.583 kb on + strand at 453.583 kb on + strand at 453.583 kb on + strand at 453.584 kb on - strand at 453.617 kb on + strand at 453.672 kb on - strand at 453.674 kb on + strand at 453.675 kb on - strand at 453.687 kb on + strand at 453.688 kb on - strand at 453.784 kb on + strand at 453.798 kb on + strand at 453.799 kb on - strand at 453.799 kb on - strand at 453.799 kb on - strand at 453.802 kb on + strand at 453.817 kb on + strand at 453.894 kb on - strand at 453.899 kb on + strand at 453.899 kb on + strand at 453.924 kb on + strand, within CA265_RS01815 at 453.938 kb on + strand, within CA265_RS01815 at 453.951 kb on - strand, within CA265_RS01815 at 453.954 kb on + strand, within CA265_RS01815 at 453.955 kb on - strand, within CA265_RS01815 at 453.955 kb on - strand, within CA265_RS01815 at 453.976 kb on - strand, within CA265_RS01815 at 453.976 kb on - strand, within CA265_RS01815 at 453.976 kb on - strand, within CA265_RS01815 at 453.984 kb on - strand, within CA265_RS01815 at 454.022 kb on + strand, within CA265_RS01815 at 454.022 kb on + strand, within CA265_RS01815 at 454.022 kb on + strand, within CA265_RS01815 at 454.023 kb on - strand, within CA265_RS01815 at 454.123 kb on + strand, within CA265_RS01815 at 454.127 kb on + strand, within CA265_RS01815 at 454.165 kb on + strand, within CA265_RS01815 at 454.182 kb on - strand, within CA265_RS01815 at 454.247 kb on + strand, within CA265_RS01815 at 454.259 kb on + strand, within CA265_RS01815 at 454.362 kb on + strand at 454.373 kb on - strand at 454.377 kb on + strand at 454.377 kb on + strand at 454.377 kb on + strand at 454.377 kb on + strand at 454.378 kb on - strand at 454.379 kb on + strand at 454.389 kb on + strand at 454.390 kb on - strand at 454.390 kb on - strand at 454.430 kb on + strand at 454.471 kb on + strand at 454.564 kb on - strand at 454.564 kb on - strand at 454.622 kb on - strand at 454.629 kb on + strand at 454.641 kb on + strand at 454.784 kb on - strand, within CA265_RS01820 at 454.784 kb on - strand, within CA265_RS01820 at 454.784 kb on - strand, within CA265_RS01820 at 454.820 kb on + strand, within CA265_RS01820 at 454.821 kb on - strand, within CA265_RS01820 at 454.849 kb on + strand, within CA265_RS01820 at 454.850 kb on - strand, within CA265_RS01820 at 454.850 kb on - strand, within CA265_RS01820 at 454.851 kb on + strand, within CA265_RS01820 at 454.852 kb on - strand, within CA265_RS01820 at 454.852 kb on - strand, within CA265_RS01820 at 454.852 kb on - strand, within CA265_RS01820 at 454.948 kb on - strand, within CA265_RS01820 at 454.957 kb on + strand, within CA265_RS01820 at 454.957 kb on + strand, within CA265_RS01820 at 454.958 kb on - strand, within CA265_RS01820 at 454.958 kb on - strand, within CA265_RS01820 at 455.010 kb on + strand, within CA265_RS01820 at 455.107 kb on + strand, within CA265_RS01820 at 455.108 kb on - strand, within CA265_RS01820 at 455.174 kb on - strand, within CA265_RS01820 at 455.181 kb on - strand, within CA265_RS01820 at 455.186 kb on - strand, within CA265_RS01820 at 455.249 kb on + strand, within CA265_RS01820 at 455.252 kb on - strand, within CA265_RS01820
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.06 mg/ml remove 452,867 + CA265_RS01810 0.53 -0.4 452,879 + CA265_RS01810 0.54 -1.6 452,913 + CA265_RS01810 0.56 -1.4 452,913 + CA265_RS01810 0.56 -1.8 452,921 + CA265_RS01810 0.56 -0.5 452,922 - CA265_RS01810 0.57 -0.5 453,007 + CA265_RS01810 0.61 -1.5 453,008 - CA265_RS01810 0.61 -0.7 453,008 - CA265_RS01810 0.61 +0.1 453,032 - CA265_RS01810 0.63 +0.4 453,032 - CA265_RS01810 0.63 +0.4 453,032 - CA265_RS01810 0.63 -0.3 453,042 - CA265_RS01810 0.63 -0.1 453,117 - CA265_RS01810 0.67 +0.1 453,117 - CA265_RS01810 0.67 -1.6 453,117 - CA265_RS01810 0.67 -0.0 453,118 + CA265_RS01810 0.68 -1.8 453,182 + CA265_RS01810 0.71 -0.3 453,183 - CA265_RS01810 0.71 +0.1 453,194 + CA265_RS01810 0.72 -1.1 453,247 + CA265_RS01810 0.75 +0.9 453,247 + CA265_RS01810 0.75 -0.4 453,248 - CA265_RS01810 0.75 -1.3 453,248 - CA265_RS01810 0.75 -0.5 453,302 + CA265_RS01810 0.78 +0.5 453,326 + CA265_RS01810 0.79 -0.3 453,373 + CA265_RS01810 0.82 -1.1 453,373 + CA265_RS01810 0.82 +0.2 453,411 - CA265_RS01810 0.84 +0.9 453,417 + CA265_RS01810 0.84 +1.9 453,418 - CA265_RS01810 0.84 +0.3 453,449 - CA265_RS01810 0.86 -0.5 453,449 - CA265_RS01810 0.86 -1.1 453,454 + CA265_RS01810 0.86 -2.0 453,469 + CA265_RS01810 0.87 -0.6 453,469 + CA265_RS01810 0.87 +2.4 453,525 - -0.8 453,583 + -0.7 453,583 + -1.6 453,583 + -0.1 453,584 - -0.1 453,617 + -0.7 453,672 - +0.1 453,674 + -0.4 453,675 - -0.6 453,687 + -1.1 453,688 - -0.2 453,784 + -0.5 453,798 + -0.8 453,799 - -0.1 453,799 - -1.8 453,799 - +2.0 453,802 + -0.9 453,817 + -1.3 453,894 - +0.0 453,899 + -0.6 453,899 + -1.0 453,924 + CA265_RS01815 0.12 +0.4 453,938 + CA265_RS01815 0.15 -1.1 453,951 - CA265_RS01815 0.17 -0.3 453,954 + CA265_RS01815 0.18 -2.5 453,955 - CA265_RS01815 0.18 -0.4 453,955 - CA265_RS01815 0.18 -0.1 453,976 - CA265_RS01815 0.22 -1.5 453,976 - CA265_RS01815 0.22 +1.9 453,976 - CA265_RS01815 0.22 -0.8 453,984 - CA265_RS01815 0.24 -0.1 454,022 + CA265_RS01815 0.31 -1.3 454,022 + CA265_RS01815 0.31 -0.2 454,022 + CA265_RS01815 0.31 +1.0 454,023 - CA265_RS01815 0.32 -1.1 454,123 + CA265_RS01815 0.51 -0.6 454,127 + CA265_RS01815 0.52 -1.7 454,165 + CA265_RS01815 0.60 -0.5 454,182 - CA265_RS01815 0.63 -2.1 454,247 + CA265_RS01815 0.76 -0.9 454,259 + CA265_RS01815 0.78 -1.0 454,362 + -1.1 454,373 - -0.4 454,377 + -0.5 454,377 + -1.3 454,377 + -0.4 454,377 + -1.1 454,378 - +0.1 454,379 + -1.3 454,389 + +0.6 454,390 - +0.7 454,390 - +0.7 454,430 + -0.9 454,471 + -0.4 454,564 - -0.9 454,564 - -0.5 454,622 - +0.9 454,629 + -0.6 454,641 + -0.5 454,784 - CA265_RS01820 0.11 +1.0 454,784 - CA265_RS01820 0.11 +0.9 454,784 - CA265_RS01820 0.11 +0.6 454,820 + CA265_RS01820 0.12 -1.0 454,821 - CA265_RS01820 0.13 +0.3 454,849 + CA265_RS01820 0.14 +1.4 454,850 - CA265_RS01820 0.14 +0.8 454,850 - CA265_RS01820 0.14 +1.1 454,851 + CA265_RS01820 0.14 -0.4 454,852 - CA265_RS01820 0.14 +0.6 454,852 - CA265_RS01820 0.14 +0.6 454,852 - CA265_RS01820 0.14 +0.4 454,948 - CA265_RS01820 0.18 +1.0 454,957 + CA265_RS01820 0.18 -1.0 454,957 + CA265_RS01820 0.18 -0.9 454,958 - CA265_RS01820 0.18 +1.4 454,958 - CA265_RS01820 0.18 +1.9 455,010 + CA265_RS01820 0.20 -0.2 455,107 + CA265_RS01820 0.24 -1.5 455,108 - CA265_RS01820 0.24 -0.3 455,174 - CA265_RS01820 0.27 +0.6 455,181 - CA265_RS01820 0.27 +0.2 455,186 - CA265_RS01820 0.28 +0.0 455,249 + CA265_RS01820 0.30 -0.1 455,252 - CA265_RS01820 0.30 +0.8
Or see this region's nucleotide sequence