Experiment: R2A with Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS21575 and CA265_RS21580 are separated by 574 nucleotides CA265_RS21580 and CA265_RS21585 are separated by 69 nucleotides CA265_RS21585 and CA265_RS21590 are separated by 139 nucleotides CA265_RS21590 and CA265_RS21595 are separated by 94 nucleotides
CA265_RS21575: CA265_RS21575 - RNA polymerase subunit sigma, at 5,311,570 to 5,312,151
_RS21575
CA265_RS21580: CA265_RS21580 - QacE family quaternary ammonium compound efflux SMR transporter, at 5,312,726 to 5,313,046
_RS21580
CA265_RS21585: CA265_RS21585 - cyclic nucleotide-binding protein, at 5,313,116 to 5,313,682
_RS21585
CA265_RS21590: CA265_RS21590 - hypothetical protein, at 5,313,822 to 5,314,187
_RS21590
CA265_RS21595: CA265_RS21595 - esterase, at 5,314,282 to 5,314,704
_RS21595
Position (kb) 5313
5314 Strain fitness (log2 ratio) -2
-1
0
1 at 5312.128 kb on + strand at 5312.182 kb on + strand at 5312.183 kb on - strand at 5312.249 kb on - strand at 5312.252 kb on - strand at 5312.413 kb on - strand at 5312.506 kb on - strand at 5312.568 kb on - strand at 5312.774 kb on - strand, within CA265_RS21580 at 5312.774 kb on - strand, within CA265_RS21580 at 5312.871 kb on + strand, within CA265_RS21580 at 5312.872 kb on - strand, within CA265_RS21580 at 5312.872 kb on - strand, within CA265_RS21580 at 5312.872 kb on - strand, within CA265_RS21580 at 5313.050 kb on - strand at 5313.069 kb on + strand at 5313.070 kb on - strand at 5313.117 kb on + strand at 5313.117 kb on + strand at 5313.168 kb on + strand at 5313.168 kb on + strand at 5313.178 kb on + strand, within CA265_RS21585 at 5313.199 kb on + strand, within CA265_RS21585 at 5313.221 kb on + strand, within CA265_RS21585 at 5313.221 kb on + strand, within CA265_RS21585 at 5313.266 kb on + strand, within CA265_RS21585 at 5313.284 kb on - strand, within CA265_RS21585 at 5313.290 kb on + strand, within CA265_RS21585 at 5313.333 kb on - strand, within CA265_RS21585 at 5313.346 kb on - strand, within CA265_RS21585 at 5313.435 kb on + strand, within CA265_RS21585 at 5313.510 kb on + strand, within CA265_RS21585 at 5313.511 kb on - strand, within CA265_RS21585 at 5313.540 kb on + strand, within CA265_RS21585 at 5313.541 kb on - strand, within CA265_RS21585 at 5313.575 kb on + strand, within CA265_RS21585 at 5313.576 kb on - strand, within CA265_RS21585 at 5313.618 kb on - strand, within CA265_RS21585 at 5313.744 kb on - strand at 5313.755 kb on - strand at 5313.755 kb on - strand at 5313.764 kb on - strand at 5313.792 kb on - strand at 5313.826 kb on + strand at 5314.046 kb on - strand, within CA265_RS21590 at 5314.170 kb on - strand at 5314.186 kb on - strand at 5314.186 kb on - strand at 5314.198 kb on + strand at 5314.198 kb on + strand at 5314.199 kb on - strand at 5314.202 kb on - strand at 5314.204 kb on + strand at 5314.205 kb on - strand at 5314.296 kb on + strand at 5314.386 kb on + strand, within CA265_RS21595 at 5314.390 kb on + strand, within CA265_RS21595 at 5314.390 kb on + strand, within CA265_RS21595 at 5314.391 kb on - strand, within CA265_RS21595 at 5314.391 kb on - strand, within CA265_RS21595 at 5314.473 kb on - strand, within CA265_RS21595 at 5314.488 kb on + strand, within CA265_RS21595 at 5314.494 kb on + strand, within CA265_RS21595 at 5314.496 kb on + strand, within CA265_RS21595 at 5314.496 kb on + strand, within CA265_RS21595 at 5314.497 kb on - strand, within CA265_RS21595 at 5314.506 kb on + strand, within CA265_RS21595 at 5314.553 kb on - strand, within CA265_RS21595
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 7.5 vol% remove 5,312,128 + +0.6 5,312,182 + -0.3 5,312,183 - -0.0 5,312,249 - -0.1 5,312,252 - -0.2 5,312,413 - -0.8 5,312,506 - +0.4 5,312,568 - -0.8 5,312,774 - CA265_RS21580 0.15 -0.2 5,312,774 - CA265_RS21580 0.15 +0.8 5,312,871 + CA265_RS21580 0.45 +0.3 5,312,872 - CA265_RS21580 0.45 -0.2 5,312,872 - CA265_RS21580 0.45 -0.7 5,312,872 - CA265_RS21580 0.45 +0.4 5,313,050 - +0.0 5,313,069 + -0.3 5,313,070 - +0.2 5,313,117 + +0.5 5,313,117 + -1.3 5,313,168 + -0.5 5,313,168 + -0.7 5,313,178 + CA265_RS21585 0.11 +1.0 5,313,199 + CA265_RS21585 0.15 +0.8 5,313,221 + CA265_RS21585 0.19 +0.1 5,313,221 + CA265_RS21585 0.19 -0.3 5,313,266 + CA265_RS21585 0.26 +0.9 5,313,284 - CA265_RS21585 0.30 +1.1 5,313,290 + CA265_RS21585 0.31 +0.5 5,313,333 - CA265_RS21585 0.38 +0.2 5,313,346 - CA265_RS21585 0.41 +1.3 5,313,435 + CA265_RS21585 0.56 +1.0 5,313,510 + CA265_RS21585 0.69 -0.1 5,313,511 - CA265_RS21585 0.70 -0.7 5,313,540 + CA265_RS21585 0.75 -0.3 5,313,541 - CA265_RS21585 0.75 -0.1 5,313,575 + CA265_RS21585 0.81 +0.2 5,313,576 - CA265_RS21585 0.81 +0.8 5,313,618 - CA265_RS21585 0.89 +0.3 5,313,744 - +0.2 5,313,755 - +0.6 5,313,755 - -0.2 5,313,764 - +0.8 5,313,792 - +0.8 5,313,826 + +1.5 5,314,046 - CA265_RS21590 0.61 +1.6 5,314,170 - +0.7 5,314,186 - -0.0 5,314,186 - +0.6 5,314,198 + -0.3 5,314,198 + +0.2 5,314,199 - +0.9 5,314,202 - -0.4 5,314,204 + -0.3 5,314,205 - +0.4 5,314,296 + +0.6 5,314,386 + CA265_RS21595 0.25 -0.2 5,314,390 + CA265_RS21595 0.26 -2.0 5,314,390 + CA265_RS21595 0.26 +1.1 5,314,391 - CA265_RS21595 0.26 -1.0 5,314,391 - CA265_RS21595 0.26 -0.7 5,314,473 - CA265_RS21595 0.45 +0.5 5,314,488 + CA265_RS21595 0.49 -0.1 5,314,494 + CA265_RS21595 0.50 -0.0 5,314,496 + CA265_RS21595 0.51 -0.2 5,314,496 + CA265_RS21595 0.51 +0.6 5,314,497 - CA265_RS21595 0.51 -0.2 5,314,506 + CA265_RS21595 0.53 -0.9 5,314,553 - CA265_RS21595 0.64 -0.2
Or see this region's nucleotide sequence