Experiment: R2A with Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS18660 and CA265_RS18665 are separated by 187 nucleotides CA265_RS18665 and CA265_RS18670 are separated by 94 nucleotides CA265_RS18670 and CA265_RS18675 are separated by 208 nucleotides CA265_RS18675 and CA265_RS18680 are separated by 173 nucleotides
CA265_RS18660: CA265_RS18660 - SAM-dependent methyltransferase, at 4,641,551 to 4,642,252
_RS18660
CA265_RS18665: CA265_RS18665 - hypothetical protein, at 4,642,440 to 4,642,619
_RS18665
CA265_RS18670: CA265_RS18670 - damage-inducible protein DinB, at 4,642,714 to 4,643,181
_RS18670
CA265_RS18675: CA265_RS18675 - hypothetical protein, at 4,643,390 to 4,643,734
_RS18675
CA265_RS18680: CA265_RS18680 - hypothetical protein, at 4,643,908 to 4,644,786
_RS18680
Position (kb)
4642
4643
4644 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4641.754 kb on + strand, within CA265_RS18660 at 4641.769 kb on - strand, within CA265_RS18660 at 4641.814 kb on - strand, within CA265_RS18660 at 4641.878 kb on - strand, within CA265_RS18660 at 4641.878 kb on - strand, within CA265_RS18660 at 4641.898 kb on - strand, within CA265_RS18660 at 4641.904 kb on - strand, within CA265_RS18660 at 4641.904 kb on - strand, within CA265_RS18660 at 4641.904 kb on - strand, within CA265_RS18660 at 4641.982 kb on - strand, within CA265_RS18660 at 4641.992 kb on - strand, within CA265_RS18660 at 4642.127 kb on - strand, within CA265_RS18660 at 4642.174 kb on + strand, within CA265_RS18660 at 4642.212 kb on + strand at 4642.231 kb on + strand at 4642.231 kb on + strand at 4642.236 kb on + strand at 4642.247 kb on + strand at 4642.312 kb on - strand at 4642.313 kb on + strand at 4642.314 kb on - strand at 4642.315 kb on + strand at 4642.315 kb on + strand at 4642.389 kb on + strand at 4642.399 kb on + strand at 4642.399 kb on + strand at 4642.400 kb on - strand at 4642.423 kb on + strand at 4642.543 kb on + strand, within CA265_RS18665 at 4642.590 kb on - strand, within CA265_RS18665 at 4642.602 kb on + strand at 4642.633 kb on - strand at 4642.704 kb on - strand at 4642.731 kb on + strand at 4642.734 kb on - strand at 4642.740 kb on + strand at 4642.742 kb on + strand at 4642.742 kb on + strand at 4642.742 kb on + strand at 4642.765 kb on + strand, within CA265_RS18670 at 4642.765 kb on + strand, within CA265_RS18670 at 4642.772 kb on - strand, within CA265_RS18670 at 4642.776 kb on + strand, within CA265_RS18670 at 4642.808 kb on + strand, within CA265_RS18670 at 4642.903 kb on - strand, within CA265_RS18670 at 4642.911 kb on + strand, within CA265_RS18670 at 4642.912 kb on - strand, within CA265_RS18670 at 4642.925 kb on + strand, within CA265_RS18670 at 4642.925 kb on + strand, within CA265_RS18670 at 4642.926 kb on - strand, within CA265_RS18670 at 4643.030 kb on + strand, within CA265_RS18670 at 4643.030 kb on + strand, within CA265_RS18670 at 4643.031 kb on - strand, within CA265_RS18670 at 4643.049 kb on + strand, within CA265_RS18670 at 4643.087 kb on - strand, within CA265_RS18670 at 4643.257 kb on - strand at 4643.288 kb on + strand at 4643.369 kb on + strand at 4643.369 kb on + strand at 4643.370 kb on - strand at 4643.391 kb on + strand at 4643.657 kb on + strand, within CA265_RS18675 at 4643.692 kb on + strand, within CA265_RS18675 at 4643.693 kb on - strand, within CA265_RS18675 at 4643.708 kb on - strand at 4643.708 kb on - strand at 4643.712 kb on + strand at 4643.734 kb on + strand at 4643.765 kb on - strand at 4643.950 kb on - strand at 4644.145 kb on + strand at 4644.145 kb on + strand, within CA265_RS18680 at 4644.146 kb on - strand, within CA265_RS18680 at 4644.146 kb on - strand, within CA265_RS18680
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 7.5 vol% remove 4,641,754 + CA265_RS18660 0.29 -0.4 4,641,769 - CA265_RS18660 0.31 -1.5 4,641,814 - CA265_RS18660 0.37 -2.2 4,641,878 - CA265_RS18660 0.47 -1.5 4,641,878 - CA265_RS18660 0.47 +2.0 4,641,898 - CA265_RS18660 0.49 +0.4 4,641,904 - CA265_RS18660 0.50 -1.6 4,641,904 - CA265_RS18660 0.50 -0.3 4,641,904 - CA265_RS18660 0.50 -1.2 4,641,982 - CA265_RS18660 0.61 -1.7 4,641,992 - CA265_RS18660 0.63 -0.1 4,642,127 - CA265_RS18660 0.82 -1.9 4,642,174 + CA265_RS18660 0.89 +0.4 4,642,212 + +0.2 4,642,231 + -1.5 4,642,231 + -1.9 4,642,236 + -0.4 4,642,247 + -0.0 4,642,312 - +0.7 4,642,313 + +1.1 4,642,314 - -0.1 4,642,315 + +0.1 4,642,315 + -1.0 4,642,389 + -0.2 4,642,399 + +0.6 4,642,399 + -1.0 4,642,400 - -0.6 4,642,423 + -0.4 4,642,543 + CA265_RS18665 0.57 +0.4 4,642,590 - CA265_RS18665 0.83 -0.8 4,642,602 + -0.2 4,642,633 - -1.0 4,642,704 - +1.0 4,642,731 + +0.2 4,642,734 - +1.7 4,642,740 + +0.1 4,642,742 + +0.1 4,642,742 + +0.5 4,642,742 + +1.0 4,642,765 + CA265_RS18670 0.11 -0.0 4,642,765 + CA265_RS18670 0.11 +0.0 4,642,772 - CA265_RS18670 0.12 +2.1 4,642,776 + CA265_RS18670 0.13 +0.9 4,642,808 + CA265_RS18670 0.20 +0.3 4,642,903 - CA265_RS18670 0.40 -1.1 4,642,911 + CA265_RS18670 0.42 +0.9 4,642,912 - CA265_RS18670 0.42 +0.0 4,642,925 + CA265_RS18670 0.45 -1.0 4,642,925 + CA265_RS18670 0.45 +0.2 4,642,926 - CA265_RS18670 0.45 +0.2 4,643,030 + CA265_RS18670 0.68 +0.7 4,643,030 + CA265_RS18670 0.68 +0.8 4,643,031 - CA265_RS18670 0.68 -0.3 4,643,049 + CA265_RS18670 0.72 -0.0 4,643,087 - CA265_RS18670 0.80 -0.7 4,643,257 - -0.9 4,643,288 + +0.4 4,643,369 + +0.1 4,643,369 + -0.2 4,643,370 - -1.0 4,643,391 + -0.4 4,643,657 + CA265_RS18675 0.77 -0.4 4,643,692 + CA265_RS18675 0.88 +0.4 4,643,693 - CA265_RS18675 0.88 +0.6 4,643,708 - -0.4 4,643,708 - -1.0 4,643,712 + +0.3 4,643,734 + -0.3 4,643,765 - -0.9 4,643,950 - +0.5 4,644,145 + +1.4 4,644,145 + CA265_RS18680 0.27 -0.8 4,644,146 - CA265_RS18680 0.27 -0.0 4,644,146 - CA265_RS18680 0.27 -0.1
Or see this region's nucleotide sequence