Experiment: R2A with Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS14415 and CA265_RS14420 are separated by 103 nucleotides CA265_RS14420 and CA265_RS14425 are separated by 142 nucleotides
CA265_RS14415: CA265_RS14415 - pseudouridine synthase, at 3,635,622 to 3,636,650
_RS14415
CA265_RS14420: CA265_RS14420 - hypothetical protein, at 3,636,754 to 3,637,782
_RS14420
CA265_RS14425: CA265_RS14425 - 1-aminocyclopropane-1-carboxylate deaminase, at 3,637,925 to 3,638,794
_RS14425
Position (kb)
3636
3637
3638 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3635.777 kb on + strand, within CA265_RS14415 at 3635.778 kb on - strand, within CA265_RS14415 at 3635.778 kb on - strand, within CA265_RS14415 at 3635.826 kb on - strand, within CA265_RS14415 at 3635.836 kb on + strand, within CA265_RS14415 at 3635.930 kb on + strand, within CA265_RS14415 at 3635.953 kb on - strand, within CA265_RS14415 at 3635.958 kb on - strand, within CA265_RS14415 at 3635.959 kb on + strand, within CA265_RS14415 at 3635.959 kb on + strand, within CA265_RS14415 at 3636.041 kb on - strand, within CA265_RS14415 at 3636.089 kb on + strand, within CA265_RS14415 at 3636.090 kb on - strand, within CA265_RS14415 at 3636.090 kb on - strand, within CA265_RS14415 at 3636.091 kb on + strand, within CA265_RS14415 at 3636.091 kb on + strand, within CA265_RS14415 at 3636.186 kb on - strand, within CA265_RS14415 at 3636.266 kb on + strand, within CA265_RS14415 at 3636.283 kb on + strand, within CA265_RS14415 at 3636.304 kb on - strand, within CA265_RS14415 at 3636.304 kb on - strand, within CA265_RS14415 at 3636.304 kb on - strand, within CA265_RS14415 at 3636.328 kb on - strand, within CA265_RS14415 at 3636.360 kb on - strand, within CA265_RS14415 at 3636.370 kb on - strand, within CA265_RS14415 at 3636.434 kb on - strand, within CA265_RS14415 at 3636.882 kb on - strand, within CA265_RS14420 at 3636.889 kb on + strand, within CA265_RS14420 at 3637.035 kb on - strand, within CA265_RS14420 at 3637.050 kb on - strand, within CA265_RS14420 at 3637.059 kb on + strand, within CA265_RS14420 at 3637.059 kb on + strand, within CA265_RS14420 at 3637.155 kb on + strand, within CA265_RS14420 at 3637.187 kb on + strand, within CA265_RS14420 at 3637.235 kb on + strand, within CA265_RS14420 at 3637.240 kb on - strand, within CA265_RS14420 at 3637.254 kb on + strand, within CA265_RS14420 at 3637.304 kb on + strand, within CA265_RS14420 at 3637.305 kb on - strand, within CA265_RS14420 at 3637.362 kb on + strand, within CA265_RS14420 at 3637.395 kb on - strand, within CA265_RS14420 at 3637.421 kb on - strand, within CA265_RS14420 at 3637.443 kb on + strand, within CA265_RS14420 at 3637.624 kb on + strand, within CA265_RS14420 at 3637.624 kb on + strand, within CA265_RS14420 at 3637.624 kb on + strand, within CA265_RS14420 at 3637.625 kb on - strand, within CA265_RS14420 at 3637.680 kb on - strand at 3637.723 kb on + strand at 3637.724 kb on - strand at 3637.726 kb on - strand at 3637.744 kb on - strand at 3637.744 kb on - strand at 3637.789 kb on + strand at 3637.798 kb on - strand at 3637.800 kb on + strand at 3637.801 kb on - strand at 3637.809 kb on - strand at 3637.812 kb on - strand at 3637.939 kb on - strand at 3637.942 kb on + strand at 3638.018 kb on - strand, within CA265_RS14425 at 3638.048 kb on + strand, within CA265_RS14425 at 3638.124 kb on + strand, within CA265_RS14425 at 3638.146 kb on + strand, within CA265_RS14425 at 3638.203 kb on - strand, within CA265_RS14425 at 3638.203 kb on - strand, within CA265_RS14425 at 3638.318 kb on - strand, within CA265_RS14425 at 3638.391 kb on - strand, within CA265_RS14425 at 3638.392 kb on - strand, within CA265_RS14425 at 3638.439 kb on + strand, within CA265_RS14425 at 3638.483 kb on - strand, within CA265_RS14425 at 3638.487 kb on + strand, within CA265_RS14425 at 3638.494 kb on - strand, within CA265_RS14425 at 3638.526 kb on + strand, within CA265_RS14425 at 3638.526 kb on + strand, within CA265_RS14425 at 3638.530 kb on + strand, within CA265_RS14425 at 3638.531 kb on - strand, within CA265_RS14425 at 3638.534 kb on + strand, within CA265_RS14425 at 3638.632 kb on + strand, within CA265_RS14425 at 3638.632 kb on + strand, within CA265_RS14425 at 3638.633 kb on - strand, within CA265_RS14425 at 3638.633 kb on - strand, within CA265_RS14425 at 3638.717 kb on - strand at 3638.769 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 7.5 vol% remove 3,635,777 + CA265_RS14415 0.15 +2.2 3,635,778 - CA265_RS14415 0.15 +1.4 3,635,778 - CA265_RS14415 0.15 +1.1 3,635,826 - CA265_RS14415 0.20 +1.5 3,635,836 + CA265_RS14415 0.21 +2.0 3,635,930 + CA265_RS14415 0.30 +2.3 3,635,953 - CA265_RS14415 0.32 +0.6 3,635,958 - CA265_RS14415 0.33 +1.5 3,635,959 + CA265_RS14415 0.33 +2.0 3,635,959 + CA265_RS14415 0.33 +2.8 3,636,041 - CA265_RS14415 0.41 +1.2 3,636,089 + CA265_RS14415 0.45 +1.8 3,636,090 - CA265_RS14415 0.45 +0.6 3,636,090 - CA265_RS14415 0.45 +1.3 3,636,091 + CA265_RS14415 0.46 +1.7 3,636,091 + CA265_RS14415 0.46 +3.3 3,636,186 - CA265_RS14415 0.55 +1.3 3,636,266 + CA265_RS14415 0.63 +2.5 3,636,283 + CA265_RS14415 0.64 +2.1 3,636,304 - CA265_RS14415 0.66 +1.8 3,636,304 - CA265_RS14415 0.66 +1.6 3,636,304 - CA265_RS14415 0.66 +1.6 3,636,328 - CA265_RS14415 0.69 +1.4 3,636,360 - CA265_RS14415 0.72 +1.5 3,636,370 - CA265_RS14415 0.73 +1.5 3,636,434 - CA265_RS14415 0.79 +1.9 3,636,882 - CA265_RS14420 0.12 -0.6 3,636,889 + CA265_RS14420 0.13 -0.5 3,637,035 - CA265_RS14420 0.27 -0.1 3,637,050 - CA265_RS14420 0.29 -0.0 3,637,059 + CA265_RS14420 0.30 -0.3 3,637,059 + CA265_RS14420 0.30 -0.5 3,637,155 + CA265_RS14420 0.39 -0.5 3,637,187 + CA265_RS14420 0.42 -1.0 3,637,235 + CA265_RS14420 0.47 -0.3 3,637,240 - CA265_RS14420 0.47 -0.6 3,637,254 + CA265_RS14420 0.49 -0.2 3,637,304 + CA265_RS14420 0.53 -0.6 3,637,305 - CA265_RS14420 0.54 -0.5 3,637,362 + CA265_RS14420 0.59 -0.7 3,637,395 - CA265_RS14420 0.62 +0.6 3,637,421 - CA265_RS14420 0.65 -0.4 3,637,443 + CA265_RS14420 0.67 -1.4 3,637,624 + CA265_RS14420 0.85 -2.4 3,637,624 + CA265_RS14420 0.85 +0.5 3,637,624 + CA265_RS14420 0.85 +0.3 3,637,625 - CA265_RS14420 0.85 +0.2 3,637,680 - -1.1 3,637,723 + -1.8 3,637,724 - -0.6 3,637,726 - -0.7 3,637,744 - +0.3 3,637,744 - +0.7 3,637,789 + +0.7 3,637,798 - +1.6 3,637,800 + +0.4 3,637,801 - -2.2 3,637,809 - -0.8 3,637,812 - +0.3 3,637,939 - -0.0 3,637,942 + +0.6 3,638,018 - CA265_RS14425 0.11 +0.1 3,638,048 + CA265_RS14425 0.14 -0.7 3,638,124 + CA265_RS14425 0.23 -0.4 3,638,146 + CA265_RS14425 0.25 -0.0 3,638,203 - CA265_RS14425 0.32 -0.1 3,638,203 - CA265_RS14425 0.32 -0.1 3,638,318 - CA265_RS14425 0.45 +0.1 3,638,391 - CA265_RS14425 0.54 +1.1 3,638,392 - CA265_RS14425 0.54 -0.5 3,638,439 + CA265_RS14425 0.59 -0.5 3,638,483 - CA265_RS14425 0.64 +0.7 3,638,487 + CA265_RS14425 0.65 +0.6 3,638,494 - CA265_RS14425 0.65 +0.6 3,638,526 + CA265_RS14425 0.69 -0.7 3,638,526 + CA265_RS14425 0.69 -1.2 3,638,530 + CA265_RS14425 0.70 +0.9 3,638,531 - CA265_RS14425 0.70 -0.5 3,638,534 + CA265_RS14425 0.70 -0.9 3,638,632 + CA265_RS14425 0.81 -0.4 3,638,632 + CA265_RS14425 0.81 +0.6 3,638,633 - CA265_RS14425 0.81 -1.4 3,638,633 - CA265_RS14425 0.81 -0.6 3,638,717 - +0.4 3,638,769 + +0.0
Or see this region's nucleotide sequence