Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02967 and CCNA_03902 are separated by 58 nucleotides CCNA_03902 and CCNA_03883 overlap by 4 nucleotides CCNA_03883 and CCNA_02969 are separated by 68 nucleotides CCNA_02969 and CCNA_02970 are separated by 42 nucleotides CCNA_02970 and CCNA_02971 are separated by 1 nucleotides
CCNA_02967: CCNA_02967 - PAS-family sensor histidine kinase, at 3,122,780 to 3,125,179
_02967
CCNA_03902: CCNA_03902 - hypothetical protein, at 3,125,238 to 3,125,573
_03902
CCNA_03883: CCNA_03883 - hypothetical protein, at 3,125,570 to 3,125,986
_03883
CCNA_02969: CCNA_02969 - TetR-family transcriptional regulator, at 3,126,055 to 3,126,609
_02969
CCNA_02970: CCNA_02970 - putative thiol-disulfide oxidoreductase, at 3,126,652 to 3,127,023
_02970
CCNA_02971: CCNA_02971 - membrane permease, at 3,127,025 to 3,128,053
_02971
Position (kb)
3126
3127 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3125.067 kb on - strand at 3125.074 kb on - strand at 3125.081 kb on + strand at 3125.081 kb on + strand at 3125.083 kb on - strand at 3125.089 kb on - strand at 3125.150 kb on - strand at 3125.153 kb on + strand at 3125.171 kb on - strand at 3125.195 kb on - strand at 3125.244 kb on - strand at 3125.326 kb on + strand, within CCNA_03902 at 3125.328 kb on + strand, within CCNA_03902 at 3125.334 kb on - strand, within CCNA_03902 at 3125.334 kb on - strand, within CCNA_03902 at 3125.351 kb on + strand, within CCNA_03902 at 3125.351 kb on + strand, within CCNA_03902 at 3125.351 kb on + strand, within CCNA_03902 at 3125.351 kb on + strand, within CCNA_03902 at 3125.352 kb on + strand at 3125.359 kb on - strand, within CCNA_03902 at 3125.397 kb on + strand, within CCNA_03902 at 3125.408 kb on + strand, within CCNA_03902 at 3125.433 kb on + strand, within CCNA_03902 at 3125.448 kb on + strand, within CCNA_03902 at 3125.451 kb on - strand, within CCNA_03902 at 3125.451 kb on - strand, within CCNA_03902 at 3125.521 kb on - strand, within CCNA_03902 at 3125.523 kb on + strand, within CCNA_03902 at 3125.524 kb on - strand, within CCNA_03902 at 3125.539 kb on + strand, within CCNA_03902 at 3125.544 kb on + strand at 3125.558 kb on + strand at 3125.607 kb on + strand at 3125.648 kb on + strand, within CCNA_03883 at 3125.668 kb on - strand, within CCNA_03883 at 3125.668 kb on - strand, within CCNA_03883 at 3125.760 kb on + strand, within CCNA_03883 at 3125.775 kb on + strand, within CCNA_03883 at 3125.823 kb on - strand, within CCNA_03883 at 3125.845 kb on + strand, within CCNA_03883 at 3125.848 kb on - strand, within CCNA_03883 at 3125.853 kb on - strand, within CCNA_03883 at 3125.853 kb on - strand, within CCNA_03883 at 3125.880 kb on + strand, within CCNA_03883 at 3125.893 kb on + strand, within CCNA_03883 at 3125.898 kb on + strand, within CCNA_03883 at 3125.906 kb on - strand, within CCNA_03883 at 3125.939 kb on + strand, within CCNA_03883 at 3125.939 kb on + strand, within CCNA_03883 at 3125.939 kb on + strand, within CCNA_03883 at 3125.939 kb on + strand, within CCNA_03883 at 3125.939 kb on + strand, within CCNA_03883 at 3125.939 kb on + strand, within CCNA_03883 at 3125.939 kb on + strand, within CCNA_03883 at 3125.939 kb on + strand, within CCNA_03883 at 3125.939 kb on + strand, within CCNA_03883 at 3125.947 kb on - strand at 3125.947 kb on - strand at 3125.947 kb on - strand at 3125.947 kb on - strand at 3125.947 kb on - strand at 3126.052 kb on + strand at 3126.100 kb on + strand at 3126.121 kb on + strand, within CCNA_02969 at 3126.129 kb on - strand, within CCNA_02969 at 3126.186 kb on + strand, within CCNA_02969 at 3126.204 kb on + strand, within CCNA_02969 at 3126.211 kb on + strand, within CCNA_02969 at 3126.211 kb on + strand, within CCNA_02969 at 3126.216 kb on + strand, within CCNA_02969 at 3126.226 kb on + strand, within CCNA_02969 at 3126.258 kb on + strand, within CCNA_02969 at 3126.301 kb on + strand, within CCNA_02969 at 3126.309 kb on + strand, within CCNA_02969 at 3126.323 kb on - strand, within CCNA_02969 at 3126.397 kb on + strand, within CCNA_02969 at 3126.430 kb on + strand, within CCNA_02969 at 3126.449 kb on + strand, within CCNA_02969 at 3126.502 kb on + strand, within CCNA_02969 at 3126.509 kb on + strand, within CCNA_02969 at 3126.509 kb on + strand, within CCNA_02969 at 3126.509 kb on + strand, within CCNA_02969 at 3126.509 kb on + strand, within CCNA_02969 at 3126.527 kb on + strand, within CCNA_02969 at 3126.536 kb on + strand, within CCNA_02969 at 3126.537 kb on + strand, within CCNA_02969 at 3126.585 kb on + strand at 3126.606 kb on - strand at 3126.629 kb on - strand at 3126.693 kb on - strand, within CCNA_02970 at 3126.710 kb on - strand, within CCNA_02970 at 3126.716 kb on - strand, within CCNA_02970 at 3126.725 kb on + strand, within CCNA_02970 at 3126.794 kb on + strand, within CCNA_02970 at 3126.820 kb on + strand, within CCNA_02970 at 3126.820 kb on + strand, within CCNA_02970 at 3126.920 kb on + strand, within CCNA_02970 at 3127.075 kb on + strand at 3127.092 kb on + strand at 3127.102 kb on - strand at 3127.123 kb on - strand at 3127.194 kb on - strand, within CCNA_02971 at 3127.215 kb on - strand, within CCNA_02971 at 3127.222 kb on - strand, within CCNA_02971 at 3127.233 kb on + strand, within CCNA_02971 at 3127.336 kb on + strand, within CCNA_02971 at 3127.336 kb on + strand, within CCNA_02971 at 3127.336 kb on + strand, within CCNA_02971 at 3127.344 kb on - strand, within CCNA_02971 at 3127.344 kb on - strand, within CCNA_02971 at 3127.344 kb on - strand, within CCNA_02971 at 3127.344 kb on - strand, within CCNA_02971 at 3127.344 kb on - strand, within CCNA_02971 at 3127.388 kb on + strand, within CCNA_02971 at 3127.391 kb on - strand, within CCNA_02971 at 3127.404 kb on + strand, within CCNA_02971 at 3127.404 kb on + strand, within CCNA_02971 at 3127.404 kb on + strand, within CCNA_02971 at 3127.404 kb on + strand, within CCNA_02971 at 3127.404 kb on + strand, within CCNA_02971 at 3127.412 kb on - strand, within CCNA_02971 at 3127.412 kb on - strand, within CCNA_02971 at 3127.412 kb on - strand, within CCNA_02971 at 3127.412 kb on - strand, within CCNA_02971 at 3127.412 kb on - strand, within CCNA_02971 at 3127.412 kb on - strand, within CCNA_02971 at 3127.429 kb on + strand, within CCNA_02971 at 3127.429 kb on - strand, within CCNA_02971 at 3127.429 kb on - strand, within CCNA_02971 at 3127.429 kb on - strand, within CCNA_02971 at 3127.434 kb on + strand, within CCNA_02971 at 3127.434 kb on + strand, within CCNA_02971 at 3127.442 kb on - strand, within CCNA_02971 at 3127.453 kb on + strand, within CCNA_02971 at 3127.455 kb on - strand, within CCNA_02971 at 3127.461 kb on - strand, within CCNA_02971 at 3127.535 kb on + strand, within CCNA_02971 at 3127.538 kb on - strand, within CCNA_02971 at 3127.573 kb on - strand, within CCNA_02971 at 3127.578 kb on - strand, within CCNA_02971 at 3127.594 kb on + strand, within CCNA_02971 at 3127.599 kb on + strand, within CCNA_02971 at 3127.599 kb on + strand, within CCNA_02971
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,125,067 - -1.5 3,125,074 - -0.2 3,125,081 + +1.4 3,125,081 + -1.5 3,125,083 - +0.3 3,125,089 - +1.1 3,125,150 - -1.5 3,125,153 + -0.7 3,125,171 - +1.3 3,125,195 - +0.3 3,125,244 - +0.0 3,125,326 + CCNA_03902 0.26 -2.1 3,125,328 + CCNA_03902 0.27 +0.3 3,125,334 - CCNA_03902 0.29 -2.3 3,125,334 - CCNA_03902 0.29 -2.5 3,125,351 + CCNA_03902 0.34 +0.3 3,125,351 + CCNA_03902 0.34 -0.8 3,125,351 + CCNA_03902 0.34 -0.5 3,125,351 + CCNA_03902 0.34 -0.3 3,125,352 + +1.3 3,125,359 - CCNA_03902 0.36 -2.5 3,125,397 + CCNA_03902 0.47 +1.6 3,125,408 + CCNA_03902 0.51 -0.4 3,125,433 + CCNA_03902 0.58 +2.8 3,125,448 + CCNA_03902 0.62 +0.2 3,125,451 - CCNA_03902 0.63 -1.7 3,125,451 - CCNA_03902 0.63 -0.1 3,125,521 - CCNA_03902 0.84 -0.8 3,125,523 + CCNA_03902 0.85 -0.7 3,125,524 - CCNA_03902 0.85 -1.7 3,125,539 + CCNA_03902 0.90 -0.8 3,125,544 + -0.2 3,125,558 + +0.3 3,125,607 + -1.0 3,125,648 + CCNA_03883 0.19 -0.8 3,125,668 - CCNA_03883 0.24 -0.1 3,125,668 - CCNA_03883 0.24 +1.4 3,125,760 + CCNA_03883 0.46 -1.1 3,125,775 + CCNA_03883 0.49 +0.8 3,125,823 - CCNA_03883 0.61 -0.7 3,125,845 + CCNA_03883 0.66 -0.3 3,125,848 - CCNA_03883 0.67 +2.0 3,125,853 - CCNA_03883 0.68 +1.1 3,125,853 - CCNA_03883 0.68 -0.9 3,125,880 + CCNA_03883 0.74 +2.4 3,125,893 + CCNA_03883 0.77 +1.1 3,125,898 + CCNA_03883 0.79 -0.6 3,125,906 - CCNA_03883 0.81 -0.7 3,125,939 + CCNA_03883 0.88 +0.4 3,125,939 + CCNA_03883 0.88 +0.3 3,125,939 + CCNA_03883 0.88 +0.8 3,125,939 + CCNA_03883 0.88 +0.1 3,125,939 + CCNA_03883 0.88 -0.3 3,125,939 + CCNA_03883 0.88 -0.2 3,125,939 + CCNA_03883 0.88 +1.4 3,125,939 + CCNA_03883 0.88 -2.1 3,125,939 + CCNA_03883 0.88 -1.0 3,125,947 - -1.3 3,125,947 - -1.6 3,125,947 - +0.6 3,125,947 - +3.0 3,125,947 - -1.5 3,126,052 + -0.3 3,126,100 + -0.5 3,126,121 + CCNA_02969 0.12 -2.1 3,126,129 - CCNA_02969 0.13 -0.2 3,126,186 + CCNA_02969 0.24 -1.4 3,126,204 + CCNA_02969 0.27 -0.9 3,126,211 + CCNA_02969 0.28 -1.4 3,126,211 + CCNA_02969 0.28 -1.2 3,126,216 + CCNA_02969 0.29 +0.6 3,126,226 + CCNA_02969 0.31 -2.2 3,126,258 + CCNA_02969 0.37 -1.2 3,126,301 + CCNA_02969 0.44 -1.8 3,126,309 + CCNA_02969 0.46 -1.6 3,126,323 - CCNA_02969 0.48 -2.3 3,126,397 + CCNA_02969 0.62 -1.4 3,126,430 + CCNA_02969 0.68 -0.6 3,126,449 + CCNA_02969 0.71 -1.3 3,126,502 + CCNA_02969 0.81 -0.1 3,126,509 + CCNA_02969 0.82 +1.4 3,126,509 + CCNA_02969 0.82 -1.3 3,126,509 + CCNA_02969 0.82 -0.2 3,126,509 + CCNA_02969 0.82 +0.3 3,126,527 + CCNA_02969 0.85 +1.3 3,126,536 + CCNA_02969 0.87 +2.0 3,126,537 + CCNA_02969 0.87 +0.2 3,126,585 + -0.1 3,126,606 - -1.0 3,126,629 - -0.2 3,126,693 - CCNA_02970 0.11 -0.8 3,126,710 - CCNA_02970 0.16 -0.5 3,126,716 - CCNA_02970 0.17 +2.8 3,126,725 + CCNA_02970 0.20 -1.4 3,126,794 + CCNA_02970 0.38 -0.3 3,126,820 + CCNA_02970 0.45 -1.1 3,126,820 + CCNA_02970 0.45 +1.4 3,126,920 + CCNA_02970 0.72 +1.9 3,127,075 + +0.8 3,127,092 + +2.0 3,127,102 - +0.2 3,127,123 - +0.3 3,127,194 - CCNA_02971 0.16 -0.3 3,127,215 - CCNA_02971 0.18 -0.1 3,127,222 - CCNA_02971 0.19 -0.4 3,127,233 + CCNA_02971 0.20 -1.0 3,127,336 + CCNA_02971 0.30 -2.1 3,127,336 + CCNA_02971 0.30 -1.6 3,127,336 + CCNA_02971 0.30 -1.2 3,127,344 - CCNA_02971 0.31 +0.8 3,127,344 - CCNA_02971 0.31 +0.4 3,127,344 - CCNA_02971 0.31 -0.1 3,127,344 - CCNA_02971 0.31 -0.5 3,127,344 - CCNA_02971 0.31 +1.9 3,127,388 + CCNA_02971 0.35 -1.0 3,127,391 - CCNA_02971 0.36 +0.9 3,127,404 + CCNA_02971 0.37 -0.6 3,127,404 + CCNA_02971 0.37 -1.1 3,127,404 + CCNA_02971 0.37 -0.5 3,127,404 + CCNA_02971 0.37 -0.4 3,127,404 + CCNA_02971 0.37 -1.2 3,127,412 - CCNA_02971 0.38 +2.2 3,127,412 - CCNA_02971 0.38 +2.4 3,127,412 - CCNA_02971 0.38 +0.9 3,127,412 - CCNA_02971 0.38 +0.8 3,127,412 - CCNA_02971 0.38 +0.1 3,127,412 - CCNA_02971 0.38 -1.4 3,127,429 + CCNA_02971 0.39 -0.1 3,127,429 - CCNA_02971 0.39 -1.2 3,127,429 - CCNA_02971 0.39 -1.2 3,127,429 - CCNA_02971 0.39 -0.4 3,127,434 + CCNA_02971 0.40 -0.7 3,127,434 + CCNA_02971 0.40 -0.1 3,127,442 - CCNA_02971 0.41 -1.7 3,127,453 + CCNA_02971 0.42 -1.6 3,127,455 - CCNA_02971 0.42 +1.0 3,127,461 - CCNA_02971 0.42 +0.3 3,127,535 + CCNA_02971 0.50 -1.3 3,127,538 - CCNA_02971 0.50 -0.5 3,127,573 - CCNA_02971 0.53 -0.3 3,127,578 - CCNA_02971 0.54 -0.8 3,127,594 + CCNA_02971 0.55 +0.3 3,127,599 + CCNA_02971 0.56 -0.5 3,127,599 + CCNA_02971 0.56 +3.2
Or see this region's nucleotide sequence