Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01296 and CCNA_01297 are separated by 107 nucleotides CCNA_01297 and CCNA_R0030 are separated by 173 nucleotides CCNA_R0030 and CCNA_R0031 are separated by 110 nucleotides CCNA_R0031 and CCNA_01298 are separated by 54 nucleotides
CCNA_01296: CCNA_01296 - stalk-specific protein X, at 1,420,118 to 1,422,046
_01296
CCNA_01297: CCNA_01297 - 23S rRNA Gm2251 methyltransferase, at 1,422,154 to 1,422,990
_01297
CCNA_R0030: CCNA_R0030 - tRNA-Tyr, at 1,423,164 to 1,423,249
_R0030
CCNA_R0031: CCNA_R0031 - tRNA-Gly, at 1,423,360 to 1,423,433
_R0031
CCNA_01298: CCNA_01298 - translation elongation factor Tu (EF-TU), at 1,423,488 to 1,424,678
_01298
Position (kb)
1422
1423 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1421.157 kb on + strand, within CCNA_01296 at 1421.157 kb on + strand, within CCNA_01296 at 1421.157 kb on + strand, within CCNA_01296 at 1421.164 kb on - strand, within CCNA_01296 at 1421.218 kb on + strand, within CCNA_01296 at 1421.219 kb on + strand, within CCNA_01296 at 1421.226 kb on - strand, within CCNA_01296 at 1421.231 kb on + strand, within CCNA_01296 at 1421.238 kb on - strand, within CCNA_01296 at 1421.239 kb on - strand, within CCNA_01296 at 1421.268 kb on - strand, within CCNA_01296 at 1421.337 kb on - strand, within CCNA_01296 at 1421.369 kb on - strand, within CCNA_01296 at 1421.373 kb on - strand, within CCNA_01296 at 1421.390 kb on + strand, within CCNA_01296 at 1421.390 kb on + strand, within CCNA_01296 at 1421.437 kb on - strand, within CCNA_01296 at 1421.526 kb on - strand, within CCNA_01296 at 1421.547 kb on + strand, within CCNA_01296 at 1421.547 kb on + strand, within CCNA_01296 at 1421.547 kb on + strand, within CCNA_01296 at 1421.547 kb on + strand, within CCNA_01296 at 1421.555 kb on - strand, within CCNA_01296 at 1421.555 kb on - strand, within CCNA_01296 at 1421.600 kb on - strand, within CCNA_01296 at 1421.600 kb on - strand, within CCNA_01296 at 1421.616 kb on + strand, within CCNA_01296 at 1421.621 kb on + strand, within CCNA_01296 at 1421.626 kb on + strand, within CCNA_01296 at 1421.631 kb on + strand, within CCNA_01296 at 1421.634 kb on - strand, within CCNA_01296 at 1421.639 kb on - strand, within CCNA_01296 at 1421.643 kb on + strand, within CCNA_01296 at 1421.649 kb on + strand, within CCNA_01296 at 1421.651 kb on - strand, within CCNA_01296 at 1421.651 kb on - strand, within CCNA_01296 at 1421.697 kb on + strand, within CCNA_01296 at 1421.705 kb on - strand, within CCNA_01296 at 1421.724 kb on + strand, within CCNA_01296 at 1421.724 kb on + strand, within CCNA_01296 at 1421.724 kb on + strand, within CCNA_01296 at 1421.732 kb on - strand, within CCNA_01296 at 1421.732 kb on - strand, within CCNA_01296 at 1421.757 kb on + strand, within CCNA_01296 at 1421.757 kb on + strand, within CCNA_01296 at 1421.757 kb on + strand, within CCNA_01296 at 1421.757 kb on + strand, within CCNA_01296 at 1421.757 kb on + strand, within CCNA_01296 at 1421.764 kb on - strand, within CCNA_01296 at 1421.765 kb on - strand, within CCNA_01296 at 1421.765 kb on - strand, within CCNA_01296 at 1421.813 kb on - strand, within CCNA_01296 at 1421.817 kb on + strand, within CCNA_01296 at 1421.822 kb on + strand, within CCNA_01296 at 1421.825 kb on - strand, within CCNA_01296 at 1421.825 kb on - strand, within CCNA_01296 at 1421.825 kb on - strand, within CCNA_01296 at 1421.830 kb on - strand, within CCNA_01296 at 1421.835 kb on - strand, within CCNA_01296 at 1421.860 kb on - strand at 1421.883 kb on - strand at 1421.911 kb on - strand at 1421.955 kb on - strand at 1422.034 kb on - strand at 1422.199 kb on - strand at 1422.225 kb on - strand at 1422.226 kb on + strand at 1422.272 kb on + strand, within CCNA_01297 at 1422.325 kb on - strand, within CCNA_01297 at 1422.363 kb on - strand, within CCNA_01297 at 1422.395 kb on + strand, within CCNA_01297 at 1422.400 kb on + strand, within CCNA_01297 at 1422.405 kb on + strand, within CCNA_01297 at 1422.434 kb on + strand, within CCNA_01297 at 1422.458 kb on + strand, within CCNA_01297 at 1422.458 kb on + strand, within CCNA_01297 at 1422.459 kb on - strand, within CCNA_01297 at 1422.487 kb on + strand, within CCNA_01297 at 1422.512 kb on - strand, within CCNA_01297 at 1422.536 kb on - strand, within CCNA_01297 at 1422.537 kb on - strand, within CCNA_01297 at 1422.542 kb on + strand, within CCNA_01297 at 1422.588 kb on + strand, within CCNA_01297 at 1422.600 kb on + strand, within CCNA_01297 at 1422.608 kb on - strand, within CCNA_01297 at 1422.657 kb on + strand, within CCNA_01297 at 1422.665 kb on - strand, within CCNA_01297 at 1422.680 kb on + strand, within CCNA_01297 at 1422.681 kb on - strand, within CCNA_01297 at 1422.691 kb on - strand, within CCNA_01297 at 1422.723 kb on + strand, within CCNA_01297 at 1422.724 kb on + strand, within CCNA_01297 at 1422.724 kb on + strand, within CCNA_01297 at 1422.732 kb on - strand, within CCNA_01297 at 1422.742 kb on - strand, within CCNA_01297 at 1422.749 kb on + strand, within CCNA_01297 at 1422.808 kb on + strand, within CCNA_01297 at 1422.886 kb on + strand, within CCNA_01297 at 1422.894 kb on - strand, within CCNA_01297 at 1422.894 kb on - strand, within CCNA_01297 at 1422.894 kb on - strand, within CCNA_01297 at 1423.289 kb on + strand at 1423.297 kb on - strand at 1423.444 kb on + strand at 1423.478 kb on + strand at 1423.478 kb on + strand at 1423.486 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,421,157 + CCNA_01296 0.54 -1.9 1,421,157 + CCNA_01296 0.54 -1.5 1,421,157 + CCNA_01296 0.54 -0.7 1,421,164 - CCNA_01296 0.54 -1.1 1,421,218 + CCNA_01296 0.57 -0.2 1,421,219 + CCNA_01296 0.57 +0.3 1,421,226 - CCNA_01296 0.57 +0.5 1,421,231 + CCNA_01296 0.58 +1.0 1,421,238 - CCNA_01296 0.58 +0.8 1,421,239 - CCNA_01296 0.58 -0.2 1,421,268 - CCNA_01296 0.60 -0.0 1,421,337 - CCNA_01296 0.63 -1.6 1,421,369 - CCNA_01296 0.65 +0.5 1,421,373 - CCNA_01296 0.65 -0.8 1,421,390 + CCNA_01296 0.66 -1.3 1,421,390 + CCNA_01296 0.66 -1.1 1,421,437 - CCNA_01296 0.68 +0.9 1,421,526 - CCNA_01296 0.73 +1.1 1,421,547 + CCNA_01296 0.74 +2.7 1,421,547 + CCNA_01296 0.74 +0.9 1,421,547 + CCNA_01296 0.74 +0.8 1,421,547 + CCNA_01296 0.74 +0.5 1,421,555 - CCNA_01296 0.74 -0.5 1,421,555 - CCNA_01296 0.74 +1.4 1,421,600 - CCNA_01296 0.77 +0.8 1,421,600 - CCNA_01296 0.77 -0.6 1,421,616 + CCNA_01296 0.78 -0.6 1,421,621 + CCNA_01296 0.78 -0.8 1,421,626 + CCNA_01296 0.78 -1.5 1,421,631 + CCNA_01296 0.78 -1.5 1,421,634 - CCNA_01296 0.79 +0.8 1,421,639 - CCNA_01296 0.79 +0.1 1,421,643 + CCNA_01296 0.79 -0.7 1,421,649 + CCNA_01296 0.79 -0.8 1,421,651 - CCNA_01296 0.79 +0.8 1,421,651 - CCNA_01296 0.79 +0.5 1,421,697 + CCNA_01296 0.82 +0.8 1,421,705 - CCNA_01296 0.82 +0.7 1,421,724 + CCNA_01296 0.83 -1.8 1,421,724 + CCNA_01296 0.83 +0.1 1,421,724 + CCNA_01296 0.83 -1.9 1,421,732 - CCNA_01296 0.84 +0.4 1,421,732 - CCNA_01296 0.84 +0.1 1,421,757 + CCNA_01296 0.85 +0.3 1,421,757 + CCNA_01296 0.85 -1.7 1,421,757 + CCNA_01296 0.85 +0.2 1,421,757 + CCNA_01296 0.85 -0.1 1,421,757 + CCNA_01296 0.85 -1.1 1,421,764 - CCNA_01296 0.85 +0.4 1,421,765 - CCNA_01296 0.85 -0.5 1,421,765 - CCNA_01296 0.85 +0.1 1,421,813 - CCNA_01296 0.88 -2.1 1,421,817 + CCNA_01296 0.88 -0.4 1,421,822 + CCNA_01296 0.88 -0.6 1,421,825 - CCNA_01296 0.88 -1.8 1,421,825 - CCNA_01296 0.88 -0.3 1,421,825 - CCNA_01296 0.88 +1.5 1,421,830 - CCNA_01296 0.89 +2.4 1,421,835 - CCNA_01296 0.89 -0.7 1,421,860 - -0.6 1,421,883 - +1.2 1,421,911 - +1.1 1,421,955 - +0.3 1,422,034 - -0.6 1,422,199 - -0.0 1,422,225 - -0.5 1,422,226 + -0.2 1,422,272 + CCNA_01297 0.14 -0.2 1,422,325 - CCNA_01297 0.20 +0.6 1,422,363 - CCNA_01297 0.25 +0.8 1,422,395 + CCNA_01297 0.29 -2.1 1,422,400 + CCNA_01297 0.29 -0.1 1,422,405 + CCNA_01297 0.30 -1.9 1,422,434 + CCNA_01297 0.33 -1.5 1,422,458 + CCNA_01297 0.36 -0.3 1,422,458 + CCNA_01297 0.36 -1.0 1,422,459 - CCNA_01297 0.36 +0.6 1,422,487 + CCNA_01297 0.40 +1.8 1,422,512 - CCNA_01297 0.43 -0.9 1,422,536 - CCNA_01297 0.46 -2.6 1,422,537 - CCNA_01297 0.46 -0.8 1,422,542 + CCNA_01297 0.46 -0.4 1,422,588 + CCNA_01297 0.52 -1.0 1,422,600 + CCNA_01297 0.53 -0.3 1,422,608 - CCNA_01297 0.54 +1.4 1,422,657 + CCNA_01297 0.60 -0.2 1,422,665 - CCNA_01297 0.61 +1.4 1,422,680 + CCNA_01297 0.63 -0.8 1,422,681 - CCNA_01297 0.63 -0.7 1,422,691 - CCNA_01297 0.64 -1.1 1,422,723 + CCNA_01297 0.68 +0.2 1,422,724 + CCNA_01297 0.68 +1.1 1,422,724 + CCNA_01297 0.68 -1.0 1,422,732 - CCNA_01297 0.69 -0.1 1,422,742 - CCNA_01297 0.70 -0.6 1,422,749 + CCNA_01297 0.71 -0.9 1,422,808 + CCNA_01297 0.78 -0.2 1,422,886 + CCNA_01297 0.87 +0.7 1,422,894 - CCNA_01297 0.88 +1.1 1,422,894 - CCNA_01297 0.88 -0.3 1,422,894 - CCNA_01297 0.88 +0.3 1,423,289 + -1.5 1,423,297 - -0.0 1,423,444 + -0.0 1,423,478 + +0.6 1,423,478 + -0.1 1,423,486 - +1.1
Or see this region's nucleotide sequence