Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00225 and CCNA_00226 are separated by 100 nucleotides CCNA_00226 and CCNA_00227 are separated by 57 nucleotides CCNA_00227 and CCNA_00228 overlap by 1 nucleotides CCNA_00228 and CCNA_00229 are separated by 119 nucleotides
CCNA_00225: CCNA_00225 - hypothetical protein, at 243,024 to 243,056
_00225
CCNA_00226: CCNA_00226 - hypothetical protein, at 243,157 to 243,432
_00226
CCNA_00227: CCNA_00227 - methyltransferase, at 243,490 to 244,056
_00227
CCNA_00228: CCNA_00228 - putative membrane spanning protein, at 244,056 to 244,670
_00228
CCNA_00229: CCNA_00229 - CAAX amino terminal protease family protein, at 244,790 to 245,764
_00229
Position (kb) 244
245 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 243.068 kb on - strand at 243.083 kb on + strand at 243.091 kb on - strand at 243.154 kb on + strand at 243.162 kb on - strand at 243.162 kb on - strand at 243.162 kb on - strand at 243.162 kb on - strand at 243.164 kb on + strand at 243.164 kb on + strand at 243.164 kb on + strand at 243.198 kb on + strand, within CCNA_00226 at 243.198 kb on + strand, within CCNA_00226 at 243.202 kb on - strand, within CCNA_00226 at 243.205 kb on + strand, within CCNA_00226 at 243.206 kb on - strand, within CCNA_00226 at 243.213 kb on + strand, within CCNA_00226 at 243.246 kb on - strand, within CCNA_00226 at 243.259 kb on + strand, within CCNA_00226 at 243.275 kb on + strand, within CCNA_00226 at 243.275 kb on + strand, within CCNA_00226 at 243.276 kb on - strand, within CCNA_00226 at 243.283 kb on - strand, within CCNA_00226 at 243.304 kb on + strand, within CCNA_00226 at 243.400 kb on + strand, within CCNA_00226 at 243.424 kb on - strand at 243.431 kb on - strand at 243.434 kb on + strand at 243.449 kb on + strand at 243.449 kb on + strand at 243.449 kb on + strand at 243.457 kb on - strand at 243.457 kb on - strand at 243.457 kb on - strand at 243.457 kb on - strand at 243.462 kb on + strand at 243.470 kb on - strand at 243.479 kb on - strand at 243.489 kb on - strand at 243.489 kb on - strand at 243.495 kb on - strand at 243.528 kb on - strand at 243.539 kb on + strand at 243.547 kb on - strand, within CCNA_00227 at 243.557 kb on - strand, within CCNA_00227 at 243.557 kb on - strand, within CCNA_00227 at 243.557 kb on - strand, within CCNA_00227 at 243.598 kb on - strand, within CCNA_00227 at 243.620 kb on + strand, within CCNA_00227 at 243.647 kb on - strand, within CCNA_00227 at 243.647 kb on - strand, within CCNA_00227 at 243.673 kb on - strand, within CCNA_00227 at 243.708 kb on + strand, within CCNA_00227 at 243.729 kb on + strand, within CCNA_00227 at 243.729 kb on + strand, within CCNA_00227 at 243.729 kb on + strand, within CCNA_00227 at 243.737 kb on - strand, within CCNA_00227 at 243.751 kb on - strand, within CCNA_00227 at 243.756 kb on - strand, within CCNA_00227 at 243.756 kb on - strand, within CCNA_00227 at 243.767 kb on + strand, within CCNA_00227 at 243.783 kb on - strand, within CCNA_00227 at 243.783 kb on - strand, within CCNA_00227 at 243.844 kb on + strand, within CCNA_00227 at 243.848 kb on - strand, within CCNA_00227 at 243.869 kb on + strand, within CCNA_00227 at 243.932 kb on - strand, within CCNA_00227 at 243.932 kb on - strand, within CCNA_00227 at 243.939 kb on - strand, within CCNA_00227 at 243.955 kb on + strand, within CCNA_00227 at 243.956 kb on - strand, within CCNA_00227 at 243.977 kb on - strand, within CCNA_00227 at 244.016 kb on + strand at 244.016 kb on + strand at 244.022 kb on + strand at 244.062 kb on - strand at 244.063 kb on + strand at 244.063 kb on + strand at 244.063 kb on + strand at 244.063 kb on + strand at 244.063 kb on + strand at 244.063 kb on + strand at 244.063 kb on + strand at 244.063 kb on + strand at 244.063 kb on + strand at 244.068 kb on + strand at 244.068 kb on + strand at 244.071 kb on - strand at 244.071 kb on - strand at 244.071 kb on - strand at 244.071 kb on - strand at 244.071 kb on - strand at 244.076 kb on - strand at 244.076 kb on - strand at 244.076 kb on - strand at 244.112 kb on - strand at 244.112 kb on - strand at 244.135 kb on + strand, within CCNA_00228 at 244.146 kb on + strand, within CCNA_00228 at 244.146 kb on + strand, within CCNA_00228 at 244.154 kb on - strand, within CCNA_00228 at 244.155 kb on - strand, within CCNA_00228 at 244.155 kb on - strand, within CCNA_00228 at 244.155 kb on - strand, within CCNA_00228 at 244.160 kb on + strand, within CCNA_00228 at 244.161 kb on + strand, within CCNA_00228 at 244.187 kb on - strand, within CCNA_00228 at 244.187 kb on - strand at 244.187 kb on - strand, within CCNA_00228 at 244.223 kb on + strand, within CCNA_00228 at 244.245 kb on + strand, within CCNA_00228 at 244.294 kb on + strand, within CCNA_00228 at 244.313 kb on + strand, within CCNA_00228 at 244.321 kb on - strand, within CCNA_00228 at 244.323 kb on + strand, within CCNA_00228 at 244.323 kb on + strand, within CCNA_00228 at 244.325 kb on - strand, within CCNA_00228 at 244.327 kb on + strand, within CCNA_00228 at 244.327 kb on + strand, within CCNA_00228 at 244.332 kb on + strand, within CCNA_00228 at 244.335 kb on - strand, within CCNA_00228 at 244.335 kb on - strand, within CCNA_00228 at 244.351 kb on + strand, within CCNA_00228 at 244.359 kb on - strand, within CCNA_00228 at 244.359 kb on - strand, within CCNA_00228 at 244.369 kb on - strand, within CCNA_00228 at 244.369 kb on - strand, within CCNA_00228 at 244.369 kb on - strand, within CCNA_00228 at 244.369 kb on - strand, within CCNA_00228 at 244.392 kb on + strand, within CCNA_00228 at 244.392 kb on + strand, within CCNA_00228 at 244.392 kb on + strand, within CCNA_00228 at 244.400 kb on - strand, within CCNA_00228 at 244.400 kb on - strand, within CCNA_00228 at 244.400 kb on - strand, within CCNA_00228 at 244.405 kb on - strand, within CCNA_00228 at 244.415 kb on - strand, within CCNA_00228 at 244.415 kb on - strand, within CCNA_00228 at 244.546 kb on - strand, within CCNA_00228 at 244.589 kb on - strand, within CCNA_00228 at 244.620 kb on + strand at 244.628 kb on - strand at 244.635 kb on + strand at 244.651 kb on + strand at 244.656 kb on + strand at 244.688 kb on - strand at 244.735 kb on + strand at 244.758 kb on + strand at 244.758 kb on + strand at 244.758 kb on + strand at 244.758 kb on + strand at 244.766 kb on - strand at 244.766 kb on - strand at 244.766 kb on - strand at 244.803 kb on + strand at 244.807 kb on + strand at 244.807 kb on + strand at 244.807 kb on + strand at 244.807 kb on + strand at 244.807 kb on + strand at 244.807 kb on + strand at 244.811 kb on - strand at 244.813 kb on - strand at 244.815 kb on - strand at 244.815 kb on - strand at 244.815 kb on - strand at 244.815 kb on - strand at 244.872 kb on + strand at 244.924 kb on + strand, within CCNA_00229 at 244.927 kb on - strand, within CCNA_00229 at 244.943 kb on + strand, within CCNA_00229 at 244.950 kb on + strand, within CCNA_00229 at 244.950 kb on + strand, within CCNA_00229 at 244.951 kb on - strand, within CCNA_00229 at 244.951 kb on - strand, within CCNA_00229 at 244.951 kb on - strand, within CCNA_00229 at 244.951 kb on - strand, within CCNA_00229 at 244.951 kb on - strand, within CCNA_00229 at 244.951 kb on - strand, within CCNA_00229 at 244.975 kb on + strand, within CCNA_00229 at 245.002 kb on + strand, within CCNA_00229 at 245.054 kb on + strand, within CCNA_00229 at 245.056 kb on + strand, within CCNA_00229 at 245.060 kb on + strand, within CCNA_00229 at 245.060 kb on + strand, within CCNA_00229 at 245.060 kb on + strand, within CCNA_00229 at 245.079 kb on + strand, within CCNA_00229 at 245.093 kb on - strand, within CCNA_00229 at 245.096 kb on + strand, within CCNA_00229 at 245.194 kb on - strand, within CCNA_00229 at 245.194 kb on - strand, within CCNA_00229 at 245.194 kb on - strand, within CCNA_00229 at 245.194 kb on - strand, within CCNA_00229 at 245.196 kb on + strand, within CCNA_00229 at 245.196 kb on + strand, within CCNA_00229 at 245.201 kb on + strand, within CCNA_00229 at 245.204 kb on - strand, within CCNA_00229 at 245.204 kb on - strand, within CCNA_00229 at 245.204 kb on - strand, within CCNA_00229 at 245.206 kb on + strand, within CCNA_00229 at 245.209 kb on - strand, within CCNA_00229 at 245.209 kb on - strand, within CCNA_00229 at 245.214 kb on - strand, within CCNA_00229 at 245.215 kb on + strand, within CCNA_00229 at 245.257 kb on - strand, within CCNA_00229 at 245.270 kb on - strand, within CCNA_00229 at 245.285 kb on + strand, within CCNA_00229 at 245.297 kb on - strand, within CCNA_00229 at 245.297 kb on - strand, within CCNA_00229 at 245.307 kb on - strand, within CCNA_00229 at 245.402 kb on + strand, within CCNA_00229 at 245.402 kb on + strand, within CCNA_00229 at 245.424 kb on + strand, within CCNA_00229 at 245.441 kb on + strand, within CCNA_00229 at 245.449 kb on - strand, within CCNA_00229 at 245.476 kb on - strand, within CCNA_00229 at 245.486 kb on + strand, within CCNA_00229 at 245.494 kb on - strand, within CCNA_00229 at 245.508 kb on + strand at 245.508 kb on + strand, within CCNA_00229 at 245.513 kb on + strand, within CCNA_00229 at 245.518 kb on + strand, within CCNA_00229 at 245.518 kb on + strand, within CCNA_00229 at 245.519 kb on + strand, within CCNA_00229 at 245.521 kb on - strand, within CCNA_00229 at 245.526 kb on - strand, within CCNA_00229 at 245.537 kb on - strand, within CCNA_00229 at 245.573 kb on + strand, within CCNA_00229 at 245.573 kb on + strand, within CCNA_00229 at 245.573 kb on + strand, within CCNA_00229 at 245.620 kb on + strand, within CCNA_00229 at 245.651 kb on - strand, within CCNA_00229 at 245.656 kb on - strand, within CCNA_00229 at 245.666 kb on + strand, within CCNA_00229 at 245.666 kb on + strand, within CCNA_00229 at 245.667 kb on + strand at 245.668 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 243,068 - +0.7 243,083 + -0.7 243,091 - -1.2 243,154 + +0.7 243,162 - +2.1 243,162 - -0.2 243,162 - +0.2 243,162 - -0.3 243,164 + +0.0 243,164 + +0.7 243,164 + -0.8 243,198 + CCNA_00226 0.15 +0.9 243,198 + CCNA_00226 0.15 -0.5 243,202 - CCNA_00226 0.16 +2.5 243,205 + CCNA_00226 0.17 +1.2 243,206 - CCNA_00226 0.18 -0.6 243,213 + CCNA_00226 0.20 -0.3 243,246 - CCNA_00226 0.32 -0.9 243,259 + CCNA_00226 0.37 +0.7 243,275 + CCNA_00226 0.43 -0.3 243,275 + CCNA_00226 0.43 -0.5 243,276 - CCNA_00226 0.43 -0.3 243,283 - CCNA_00226 0.46 -0.9 243,304 + CCNA_00226 0.53 +1.7 243,400 + CCNA_00226 0.88 +0.2 243,424 - -0.1 243,431 - +1.6 243,434 + -0.6 243,449 + -0.6 243,449 + -0.3 243,449 + +2.2 243,457 - -1.4 243,457 - -0.1 243,457 - -0.8 243,457 - +0.4 243,462 + -0.6 243,470 - -0.7 243,479 - +0.0 243,489 - +0.7 243,489 - -1.1 243,495 - -0.6 243,528 - -0.1 243,539 + -1.8 243,547 - CCNA_00227 0.10 -1.4 243,557 - CCNA_00227 0.12 +0.8 243,557 - CCNA_00227 0.12 -0.0 243,557 - CCNA_00227 0.12 +0.9 243,598 - CCNA_00227 0.19 +1.8 243,620 + CCNA_00227 0.23 +1.7 243,647 - CCNA_00227 0.28 +0.9 243,647 - CCNA_00227 0.28 +0.9 243,673 - CCNA_00227 0.32 -2.2 243,708 + CCNA_00227 0.38 -0.3 243,729 + CCNA_00227 0.42 +0.2 243,729 + CCNA_00227 0.42 -1.7 243,729 + CCNA_00227 0.42 -1.7 243,737 - CCNA_00227 0.44 +0.2 243,751 - CCNA_00227 0.46 -0.5 243,756 - CCNA_00227 0.47 +1.1 243,756 - CCNA_00227 0.47 -0.3 243,767 + CCNA_00227 0.49 -2.6 243,783 - CCNA_00227 0.52 -0.6 243,783 - CCNA_00227 0.52 +1.5 243,844 + CCNA_00227 0.62 +1.5 243,848 - CCNA_00227 0.63 +0.2 243,869 + CCNA_00227 0.67 -0.7 243,932 - CCNA_00227 0.78 -0.3 243,932 - CCNA_00227 0.78 +0.5 243,939 - CCNA_00227 0.79 -2.7 243,955 + CCNA_00227 0.82 -0.1 243,956 - CCNA_00227 0.82 -1.2 243,977 - CCNA_00227 0.86 +1.1 244,016 + -1.5 244,016 + -1.1 244,022 + +0.1 244,062 - -0.5 244,063 + +0.9 244,063 + +0.7 244,063 + -0.5 244,063 + +0.9 244,063 + +2.8 244,063 + -0.2 244,063 + -0.4 244,063 + -0.2 244,063 + +1.2 244,068 + -1.6 244,068 + -1.1 244,071 - +1.8 244,071 - +0.5 244,071 - -0.2 244,071 - -0.6 244,071 - +0.1 244,076 - +2.7 244,076 - +0.5 244,076 - -1.2 244,112 - -1.7 244,112 - -0.6 244,135 + CCNA_00228 0.13 -0.6 244,146 + CCNA_00228 0.15 -0.3 244,146 + CCNA_00228 0.15 -1.1 244,154 - CCNA_00228 0.16 +0.5 244,155 - CCNA_00228 0.16 +0.9 244,155 - CCNA_00228 0.16 -1.7 244,155 - CCNA_00228 0.16 -3.2 244,160 + CCNA_00228 0.17 -1.5 244,161 + CCNA_00228 0.17 -1.2 244,187 - CCNA_00228 0.21 +0.5 244,187 - +3.5 244,187 - CCNA_00228 0.21 -1.5 244,223 + CCNA_00228 0.27 -0.5 244,245 + CCNA_00228 0.31 -0.8 244,294 + CCNA_00228 0.39 -1.9 244,313 + CCNA_00228 0.42 -0.5 244,321 - CCNA_00228 0.43 +0.8 244,323 + CCNA_00228 0.43 -2.1 244,323 + CCNA_00228 0.43 -1.0 244,325 - CCNA_00228 0.44 -1.2 244,327 + CCNA_00228 0.44 -0.2 244,327 + CCNA_00228 0.44 -0.5 244,332 + CCNA_00228 0.45 -0.6 244,335 - CCNA_00228 0.45 -0.6 244,335 - CCNA_00228 0.45 -0.9 244,351 + CCNA_00228 0.48 -0.1 244,359 - CCNA_00228 0.49 -1.1 244,359 - CCNA_00228 0.49 +1.2 244,369 - CCNA_00228 0.51 -0.1 244,369 - CCNA_00228 0.51 -1.5 244,369 - CCNA_00228 0.51 -1.0 244,369 - CCNA_00228 0.51 -0.8 244,392 + CCNA_00228 0.55 -1.6 244,392 + CCNA_00228 0.55 -0.6 244,392 + CCNA_00228 0.55 -2.0 244,400 - CCNA_00228 0.56 -0.3 244,400 - CCNA_00228 0.56 +0.9 244,400 - CCNA_00228 0.56 -2.3 244,405 - CCNA_00228 0.57 -0.5 244,415 - CCNA_00228 0.58 -1.2 244,415 - CCNA_00228 0.58 -0.1 244,546 - CCNA_00228 0.80 -0.9 244,589 - CCNA_00228 0.87 -1.6 244,620 + -0.8 244,628 - -1.8 244,635 + +0.4 244,651 + +0.7 244,656 + -0.6 244,688 - -0.2 244,735 + -0.1 244,758 + +0.9 244,758 + +1.8 244,758 + -0.4 244,758 + -0.6 244,766 - -0.3 244,766 - +0.2 244,766 - +0.5 244,803 + -0.8 244,807 + -0.4 244,807 + -0.6 244,807 + +0.1 244,807 + +0.9 244,807 + +1.9 244,807 + +0.5 244,811 - -0.7 244,813 - -1.2 244,815 - -2.8 244,815 - -0.6 244,815 - +0.1 244,815 - +0.4 244,872 + -0.8 244,924 + CCNA_00229 0.14 -2.3 244,927 - CCNA_00229 0.14 -1.1 244,943 + CCNA_00229 0.16 +1.2 244,950 + CCNA_00229 0.16 -1.1 244,950 + CCNA_00229 0.16 +0.0 244,951 - CCNA_00229 0.17 -1.9 244,951 - CCNA_00229 0.17 -1.2 244,951 - CCNA_00229 0.17 +1.2 244,951 - CCNA_00229 0.17 -2.1 244,951 - CCNA_00229 0.17 +0.1 244,951 - CCNA_00229 0.17 -2.3 244,975 + CCNA_00229 0.19 +0.1 245,002 + CCNA_00229 0.22 +1.1 245,054 + CCNA_00229 0.27 +1.9 245,056 + CCNA_00229 0.27 +0.9 245,060 + CCNA_00229 0.28 -2.0 245,060 + CCNA_00229 0.28 -0.8 245,060 + CCNA_00229 0.28 -1.3 245,079 + CCNA_00229 0.30 -1.0 245,093 - CCNA_00229 0.31 -3.5 245,096 + CCNA_00229 0.31 -0.9 245,194 - CCNA_00229 0.41 -1.3 245,194 - CCNA_00229 0.41 -0.6 245,194 - CCNA_00229 0.41 +0.9 245,194 - CCNA_00229 0.41 -0.4 245,196 + CCNA_00229 0.42 +0.7 245,196 + CCNA_00229 0.42 -2.0 245,201 + CCNA_00229 0.42 -0.9 245,204 - CCNA_00229 0.42 +0.6 245,204 - CCNA_00229 0.42 +0.4 245,204 - CCNA_00229 0.42 -2.1 245,206 + CCNA_00229 0.43 -0.6 245,209 - CCNA_00229 0.43 +0.9 245,209 - CCNA_00229 0.43 +0.4 245,214 - CCNA_00229 0.43 -2.5 245,215 + CCNA_00229 0.44 -0.7 245,257 - CCNA_00229 0.48 -0.8 245,270 - CCNA_00229 0.49 -0.2 245,285 + CCNA_00229 0.51 +1.2 245,297 - CCNA_00229 0.52 +0.7 245,297 - CCNA_00229 0.52 -1.6 245,307 - CCNA_00229 0.53 +0.2 245,402 + CCNA_00229 0.63 +0.1 245,402 + CCNA_00229 0.63 -0.5 245,424 + CCNA_00229 0.65 -0.2 245,441 + CCNA_00229 0.67 -1.3 245,449 - CCNA_00229 0.68 -0.6 245,476 - CCNA_00229 0.70 -0.5 245,486 + CCNA_00229 0.71 -0.3 245,494 - CCNA_00229 0.72 -0.5 245,508 + +0.9 245,508 + CCNA_00229 0.74 -2.2 245,513 + CCNA_00229 0.74 -1.6 245,518 + CCNA_00229 0.75 -0.7 245,518 + CCNA_00229 0.75 -0.3 245,519 + CCNA_00229 0.75 -0.2 245,521 - CCNA_00229 0.75 +2.3 245,526 - CCNA_00229 0.75 +2.9 245,537 - CCNA_00229 0.77 -1.4 245,573 + CCNA_00229 0.80 -0.3 245,573 + CCNA_00229 0.80 -1.8 245,573 + CCNA_00229 0.80 -2.9 245,620 + CCNA_00229 0.85 +0.9 245,651 - CCNA_00229 0.88 -1.4 245,656 - CCNA_00229 0.89 -1.5 245,666 + CCNA_00229 0.90 +0.9 245,666 + CCNA_00229 0.90 -0.3 245,667 + -1.0 245,668 + -2.3
Or see this region's nucleotide sequence