Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS07240 and CA265_RS07245 are separated by 97 nucleotides CA265_RS07245 and CA265_RS07250 are separated by 23 nucleotides CA265_RS07250 and CA265_RS07255 are separated by 171 nucleotides CA265_RS07255 and CA265_RS07260 overlap by 4 nucleotides
CA265_RS07240: CA265_RS07240 - DUF1826 domain-containing protein, at 1,815,165 to 1,815,863
_RS07240
CA265_RS07245: CA265_RS07245 - hypothetical protein, at 1,815,961 to 1,816,668
_RS07245
CA265_RS07250: CA265_RS07250 - hypothetical protein, at 1,816,692 to 1,817,651
_RS07250
CA265_RS07255: CA265_RS07255 - aspartyl beta-hydroxylase, at 1,817,823 to 1,818,512
_RS07255
CA265_RS07260: CA265_RS07260 - hypothetical protein, at 1,818,509 to 1,819,465
_RS07260
Position (kb)
1816
1817
1818 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1815.739 kb on + strand, within CA265_RS07240 at 1815.775 kb on + strand, within CA265_RS07240 at 1815.776 kb on - strand, within CA265_RS07240 at 1815.807 kb on - strand at 1815.848 kb on + strand at 1815.876 kb on - strand at 1816.008 kb on - strand at 1816.008 kb on - strand at 1816.073 kb on - strand, within CA265_RS07245 at 1816.099 kb on - strand, within CA265_RS07245 at 1816.209 kb on + strand, within CA265_RS07245 at 1816.268 kb on + strand, within CA265_RS07245 at 1816.269 kb on - strand, within CA265_RS07245 at 1816.274 kb on - strand, within CA265_RS07245 at 1816.292 kb on + strand, within CA265_RS07245 at 1816.293 kb on - strand, within CA265_RS07245 at 1816.486 kb on + strand, within CA265_RS07245 at 1816.540 kb on - strand, within CA265_RS07245 at 1816.605 kb on + strand at 1816.605 kb on + strand at 1816.626 kb on + strand at 1816.661 kb on + strand at 1816.661 kb on + strand at 1816.661 kb on + strand at 1816.707 kb on - strand at 1816.718 kb on - strand at 1816.718 kb on - strand at 1816.788 kb on + strand, within CA265_RS07250 at 1816.821 kb on + strand, within CA265_RS07250 at 1816.916 kb on + strand, within CA265_RS07250 at 1816.956 kb on + strand, within CA265_RS07250 at 1816.972 kb on + strand, within CA265_RS07250 at 1816.972 kb on + strand, within CA265_RS07250 at 1817.085 kb on - strand, within CA265_RS07250 at 1817.118 kb on - strand at 1817.118 kb on - strand, within CA265_RS07250 at 1817.188 kb on - strand, within CA265_RS07250 at 1817.344 kb on - strand, within CA265_RS07250 at 1817.378 kb on + strand, within CA265_RS07250 at 1817.472 kb on - strand, within CA265_RS07250 at 1817.486 kb on + strand, within CA265_RS07250 at 1817.529 kb on - strand, within CA265_RS07250 at 1817.680 kb on - strand at 1817.686 kb on + strand at 1817.687 kb on - strand at 1817.795 kb on - strand at 1817.832 kb on + strand at 1817.833 kb on - strand at 1817.854 kb on - strand at 1817.893 kb on + strand, within CA265_RS07255 at 1817.987 kb on + strand, within CA265_RS07255 at 1817.988 kb on - strand, within CA265_RS07255 at 1818.001 kb on - strand, within CA265_RS07255 at 1818.008 kb on - strand, within CA265_RS07255 at 1818.053 kb on + strand, within CA265_RS07255 at 1818.054 kb on - strand, within CA265_RS07255 at 1818.057 kb on + strand, within CA265_RS07255 at 1818.179 kb on - strand, within CA265_RS07255 at 1818.179 kb on - strand, within CA265_RS07255 at 1818.182 kb on + strand, within CA265_RS07255 at 1818.182 kb on + strand, within CA265_RS07255 at 1818.183 kb on - strand, within CA265_RS07255 at 1818.183 kb on - strand, within CA265_RS07255 at 1818.262 kb on - strand, within CA265_RS07255 at 1818.288 kb on - strand, within CA265_RS07255 at 1818.328 kb on + strand, within CA265_RS07255 at 1818.328 kb on + strand, within CA265_RS07255 at 1818.337 kb on + strand, within CA265_RS07255 at 1818.379 kb on - strand, within CA265_RS07255 at 1818.381 kb on - strand, within CA265_RS07255 at 1818.381 kb on - strand, within CA265_RS07255 at 1818.430 kb on + strand, within CA265_RS07255
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 1,815,739 + CA265_RS07240 0.82 +0.6 1,815,775 + CA265_RS07240 0.87 +0.1 1,815,776 - CA265_RS07240 0.87 -0.6 1,815,807 - -0.2 1,815,848 + +0.5 1,815,876 - +0.2 1,816,008 - -0.3 1,816,008 - +0.7 1,816,073 - CA265_RS07245 0.16 +0.1 1,816,099 - CA265_RS07245 0.19 +2.0 1,816,209 + CA265_RS07245 0.35 -0.3 1,816,268 + CA265_RS07245 0.43 -0.3 1,816,269 - CA265_RS07245 0.44 +0.3 1,816,274 - CA265_RS07245 0.44 -0.8 1,816,292 + CA265_RS07245 0.47 +0.4 1,816,293 - CA265_RS07245 0.47 +0.2 1,816,486 + CA265_RS07245 0.74 +0.7 1,816,540 - CA265_RS07245 0.82 +0.2 1,816,605 + +0.2 1,816,605 + +0.0 1,816,626 + -0.8 1,816,661 + -0.2 1,816,661 + -0.1 1,816,661 + +0.5 1,816,707 - +0.5 1,816,718 - +0.2 1,816,718 - +0.8 1,816,788 + CA265_RS07250 0.10 +0.5 1,816,821 + CA265_RS07250 0.13 -0.3 1,816,916 + CA265_RS07250 0.23 -0.2 1,816,956 + CA265_RS07250 0.28 +0.4 1,816,972 + CA265_RS07250 0.29 +0.2 1,816,972 + CA265_RS07250 0.29 -1.0 1,817,085 - CA265_RS07250 0.41 -0.3 1,817,118 - +1.2 1,817,118 - CA265_RS07250 0.44 -0.7 1,817,188 - CA265_RS07250 0.52 -0.5 1,817,344 - CA265_RS07250 0.68 -1.1 1,817,378 + CA265_RS07250 0.71 -1.0 1,817,472 - CA265_RS07250 0.81 -0.2 1,817,486 + CA265_RS07250 0.83 -0.5 1,817,529 - CA265_RS07250 0.87 -0.2 1,817,680 - -0.2 1,817,686 + -0.9 1,817,687 - +0.8 1,817,795 - -0.4 1,817,832 + +0.5 1,817,833 - +0.4 1,817,854 - -2.2 1,817,893 + CA265_RS07255 0.10 -0.1 1,817,987 + CA265_RS07255 0.24 +1.0 1,817,988 - CA265_RS07255 0.24 +0.6 1,818,001 - CA265_RS07255 0.26 -1.1 1,818,008 - CA265_RS07255 0.27 -0.1 1,818,053 + CA265_RS07255 0.33 -0.3 1,818,054 - CA265_RS07255 0.33 -0.0 1,818,057 + CA265_RS07255 0.34 -0.6 1,818,179 - CA265_RS07255 0.52 +0.4 1,818,179 - CA265_RS07255 0.52 +0.2 1,818,182 + CA265_RS07255 0.52 +0.4 1,818,182 + CA265_RS07255 0.52 -0.2 1,818,183 - CA265_RS07255 0.52 -0.1 1,818,183 - CA265_RS07255 0.52 +0.3 1,818,262 - CA265_RS07255 0.64 +0.7 1,818,288 - CA265_RS07255 0.67 -0.4 1,818,328 + CA265_RS07255 0.73 -0.2 1,818,328 + CA265_RS07255 0.73 +0.2 1,818,337 + CA265_RS07255 0.74 -0.3 1,818,379 - CA265_RS07255 0.81 +0.3 1,818,381 - CA265_RS07255 0.81 -0.2 1,818,381 - CA265_RS07255 0.81 +0.1 1,818,430 + CA265_RS07255 0.88 +0.2
Or see this region's nucleotide sequence