Experiment: 1664 hydathodes 6 dpi (Dna extract from 8 * 208 hydathodes and pooled together)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Xcc-8004.3824.1 and Xcc-8004.3825.1 are separated by 101 nucleotides Xcc-8004.3825.1 and Xcc-8004.3827.1 are separated by 91 nucleotides Xcc-8004.3827.1 and Xcc-8004.3828.1 are separated by 250 nucleotides Xcc-8004.3828.1 and Xcc-8004.3829.1 are separated by 12 nucleotides
Xcc-8004.3824.1: Xcc-8004.3824.1 - Proposed peptidoglycan lipid II flippase MurJ, at 3,701,503 to 3,703,110
Xcc-8004.3824.1
Xcc-8004.3825.1: Xcc-8004.3825.1 - SSU ribosomal protein S20p, at 3,703,212 to 3,703,481
Xcc-8004.3825.1
Xcc-8004.3827.1: Xcc-8004.3827.1 - GTP-binding protein Obg, at 3,703,573 to 3,704,625
Xcc-8004.3827.1
Xcc-8004.3828.1: Xcc-8004.3828.1 - LSU ribosomal protein L27p, at 3,704,876 to 3,705,136
Xcc-8004.3828.1
Xcc-8004.3829.1: Xcc-8004.3829.1 - LSU ribosomal protein L21p, at 3,705,149 to 3,705,469
Xcc-8004.3829.1
Position (kb)
3703
3704
3705 Strain fitness (log2 ratio)
-1
0
1
2 at 3703.479 kb on + strand at 3703.479 kb on + strand at 3703.480 kb on - strand at 3704.720 kb on - strand at 3704.720 kb on - strand at 3704.720 kb on - strand at 3704.770 kb on - strand at 3704.841 kb on - strand at 3704.841 kb on - strand at 3704.878 kb on - strand at 3705.539 kb on - strand at 3705.539 kb on - strand at 3705.547 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1664 hydathodes 6 dpi (Dna extract from 8 * 208 hydathodes and pooled together) remove 3,703,479 + -0.5 3,703,479 + -0.3 3,703,480 - +0.7 3,704,720 - +0.5 3,704,720 - -0.9 3,704,720 - -0.9 3,704,770 - -0.5 3,704,841 - +2.7 3,704,841 - +0.5 3,704,878 - -1.4 3,705,539 - +0.1 3,705,539 - +0.3 3,705,547 - +0.2
Or see this region's nucleotide sequence