Experiment: 1664 hydathodes 6 dpi (Dna extract from 8 * 208 hydathodes and pooled together)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Xcc-8004.3539.1 and Xcc-8004.3540.1 are separated by 39 nucleotides Xcc-8004.3540.1 and Xcc-8004.3541.1 overlap by 4 nucleotides Xcc-8004.3541.1 and Xcc-8004.3542.1 overlap by 4 nucleotides Xcc-8004.3542.1 and Xcc-8004.3543.1 are separated by 20 nucleotides
Xcc-8004.3539.1: Xcc-8004.3539.1 - N-succinyl-L,L-diaminopimelate desuccinylase (EC 3.5.1.18), at 3,430,819 to 3,432,018
Xcc-8004.3539.1
Xcc-8004.3540.1: Xcc-8004.3540.1 - FIG138056: a glutathione-dependent thiol reductase, at 3,432,058 to 3,432,414
Xcc-8004.3540.1
Xcc-8004.3541.1: Xcc-8004.3541.1 - putative; ORF located using Glimmer/Genemark, at 3,432,411 to 3,432,884
Xcc-8004.3541.1
Xcc-8004.3542.1: Xcc-8004.3542.1 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (EC 2.3.1.117), at 3,432,881 to 3,434,053
Xcc-8004.3542.1
Xcc-8004.3543.1: Xcc-8004.3543.1 - [Protein-PII] uridylyltransferase (EC 2.7.7.59), at 3,434,074 to 3,436,683
Xcc-8004.3543.1
Position (kb)
3432
3433
3434
3435 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3432.335 kb on + strand, within Xcc-8004.3540.1 at 3432.382 kb on - strand at 3432.403 kb on + strand at 3432.404 kb on - strand at 3432.404 kb on - strand at 3432.465 kb on - strand, within Xcc-8004.3541.1 at 3432.686 kb on - strand, within Xcc-8004.3541.1 at 3432.781 kb on + strand, within Xcc-8004.3541.1 at 3432.781 kb on + strand, within Xcc-8004.3541.1 at 3432.782 kb on - strand, within Xcc-8004.3541.1 at 3432.782 kb on - strand, within Xcc-8004.3541.1 at 3432.782 kb on - strand, within Xcc-8004.3541.1 at 3432.878 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1664 hydathodes 6 dpi (Dna extract from 8 * 208 hydathodes and pooled together) remove 3,432,335 + Xcc-8004.3540.1 0.78 +0.7 3,432,382 - +1.2 3,432,403 + -0.3 3,432,404 - +0.6 3,432,404 - -1.0 3,432,465 - Xcc-8004.3541.1 0.11 -3.6 3,432,686 - Xcc-8004.3541.1 0.58 +2.2 3,432,781 + Xcc-8004.3541.1 0.78 +1.4 3,432,781 + Xcc-8004.3541.1 0.78 +0.6 3,432,782 - Xcc-8004.3541.1 0.78 -0.1 3,432,782 - Xcc-8004.3541.1 0.78 -3.0 3,432,782 - Xcc-8004.3541.1 0.78 +0.3 3,432,878 + -0.6
Or see this region's nucleotide sequence