Experiment: 1664 hydathodes 6 dpi (Dna extract from 8 * 208 hydathodes and pooled together)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Xcc-8004.245.1 and Xcc-8004.247.1 are separated by 172 nucleotides Xcc-8004.247.1 and Xcc-8004.248.1 are separated by 287 nucleotides Xcc-8004.248.1 and Xcc-8004.249.1 overlap by 11 nucleotides
Xcc-8004.245.1: Xcc-8004.245.1 - Undecaprenyl-diphosphatase (EC 3.6.1.27), at 240,872 to 241,714
Xcc-8004.245.1
Xcc-8004.247.1: Xcc-8004.247.1 - Glutamine synthetase type I (EC 6.3.1.2), at 241,887 to 243,296
Xcc-8004.247.1
Xcc-8004.248.1: Xcc-8004.248.1 - Nitrogen regulatory protein P-II, at 243,584 to 243,922
Xcc-8004.248.1
Xcc-8004.249.1: Xcc-8004.249.1 - Ammonium transporter, at 243,912 to 245,387
Xcc-8004.249.1
Position (kb) 241
242
243
244 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 241.699 kb on + strand at 241.699 kb on + strand at 241.700 kb on - strand at 241.744 kb on + strand at 241.842 kb on + strand at 241.842 kb on + strand at 241.842 kb on + strand at 241.842 kb on + strand at 241.842 kb on + strand at 241.842 kb on + strand at 241.842 kb on + strand at 241.845 kb on + strand at 241.863 kb on + strand at 241.863 kb on + strand at 241.863 kb on + strand at 241.863 kb on + strand at 241.863 kb on + strand at 241.863 kb on + strand at 241.863 kb on + strand at 241.863 kb on + strand at 241.863 kb on + strand at 241.863 kb on + strand at 241.863 kb on + strand at 241.863 kb on + strand at 243.401 kb on + strand at 243.401 kb on + strand at 243.402 kb on - strand at 243.402 kb on - strand at 243.402 kb on - strand at 243.402 kb on - strand at 243.402 kb on - strand at 243.402 kb on - strand at 243.577 kb on + strand at 243.578 kb on - strand at 243.665 kb on - strand, within Xcc-8004.248.1 at 243.719 kb on + strand, within Xcc-8004.248.1 at 243.720 kb on - strand, within Xcc-8004.248.1 at 243.720 kb on - strand, within Xcc-8004.248.1 at 243.734 kb on + strand, within Xcc-8004.248.1 at 243.734 kb on + strand, within Xcc-8004.248.1 at 243.734 kb on + strand, within Xcc-8004.248.1 at 243.734 kb on + strand, within Xcc-8004.248.1 at 243.734 kb on + strand, within Xcc-8004.248.1 at 243.735 kb on - strand, within Xcc-8004.248.1 at 243.735 kb on - strand, within Xcc-8004.248.1 at 243.735 kb on - strand, within Xcc-8004.248.1 at 243.735 kb on - strand, within Xcc-8004.248.1 at 243.735 kb on - strand, within Xcc-8004.248.1 at 243.887 kb on - strand, within Xcc-8004.248.1 at 243.944 kb on + strand at 243.945 kb on - strand at 243.945 kb on - strand at 243.984 kb on + strand at 243.985 kb on - strand at 243.985 kb on - strand at 243.985 kb on - strand at 244.242 kb on + strand, within Xcc-8004.249.1 at 244.242 kb on + strand, within Xcc-8004.249.1 at 244.242 kb on + strand, within Xcc-8004.249.1 at 244.242 kb on + strand, within Xcc-8004.249.1 at 244.242 kb on + strand, within Xcc-8004.249.1 at 244.242 kb on + strand, within Xcc-8004.249.1 at 244.242 kb on + strand, within Xcc-8004.249.1 at 244.243 kb on - strand, within Xcc-8004.249.1
Per-strain Table
Position Strand Gene LocusTag Fraction 1664 hydathodes 6 dpi (Dna extract from 8 * 208 hydathodes and pooled together) remove 241,699 + -0.8 241,699 + +0.5 241,700 - -1.2 241,744 + +0.8 241,842 + +0.5 241,842 + -0.7 241,842 + -0.1 241,842 + -1.1 241,842 + +0.9 241,842 + -1.1 241,842 + -1.7 241,845 + -1.2 241,863 + -2.4 241,863 + -0.1 241,863 + -1.7 241,863 + -0.1 241,863 + +0.9 241,863 + +0.6 241,863 + -0.1 241,863 + -4.2 241,863 + +0.9 241,863 + -2.7 241,863 + +0.3 241,863 + -1.6 243,401 + -0.1 243,401 + -3.3 243,402 - +0.6 243,402 - -2.3 243,402 - -2.8 243,402 - -1.5 243,402 - -1.4 243,402 - -1.7 243,577 + -0.1 243,578 - -2.4 243,665 - Xcc-8004.248.1 0.24 +0.9 243,719 + Xcc-8004.248.1 0.40 +0.9 243,720 - Xcc-8004.248.1 0.40 -3.0 243,720 - Xcc-8004.248.1 0.40 -2.5 243,734 + Xcc-8004.248.1 0.44 -0.2 243,734 + Xcc-8004.248.1 0.44 -2.3 243,734 + Xcc-8004.248.1 0.44 -2.6 243,734 + Xcc-8004.248.1 0.44 -0.6 243,734 + Xcc-8004.248.1 0.44 -1.8 243,735 - Xcc-8004.248.1 0.45 -3.3 243,735 - Xcc-8004.248.1 0.45 -4.0 243,735 - Xcc-8004.248.1 0.45 -1.4 243,735 - Xcc-8004.248.1 0.45 +0.4 243,735 - Xcc-8004.248.1 0.45 -0.7 243,887 - Xcc-8004.248.1 0.89 -0.1 243,944 + -1.0 243,945 - -0.3 243,945 - -1.2 243,984 + -0.9 243,985 - -2.5 243,985 - -3.1 243,985 - -0.7 244,242 + Xcc-8004.249.1 0.22 -2.1 244,242 + Xcc-8004.249.1 0.22 -0.4 244,242 + Xcc-8004.249.1 0.22 -1.9 244,242 + Xcc-8004.249.1 0.22 -2.9 244,242 + Xcc-8004.249.1 0.22 -3.5 244,242 + Xcc-8004.249.1 0.22 -0.7 244,242 + Xcc-8004.249.1 0.22 +0.7 244,243 - Xcc-8004.249.1 0.22 -1.0
Or see this region's nucleotide sequence