Experiment: 1664 hydathodes 6 dpi (Dna extract from 8 * 208 hydathodes and pooled together)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Xcc-8004.1978.1 and Xcc-8004.1979.1 are separated by 112 nucleotides Xcc-8004.1979.1 and Xcc-8004.1980.1 are separated by 33 nucleotides Xcc-8004.1980.1 and Xcc-8004.1983.1 are separated by 194 nucleotides Xcc-8004.1983.1 and Xcc-8004.1984.1 overlap by 10 nucleotides
Xcc-8004.1978.1: Xcc-8004.1978.1 - Short-chain dehydrogenase/reductase SDR, at 1,906,052 to 1,906,867
Xcc-8004.1978.1
Xcc-8004.1979.1: Xcc-8004.1979.1 - Triosephosphate isomerase (EC 5.3.1.1), at 1,906,980 to 1,907,735
Xcc-8004.1979.1
Xcc-8004.1980.1: Xcc-8004.1980.1 - Preprotein translocase subunit SecG (TC 3.A.5.1.1), at 1,907,769 to 1,908,257
Xcc-8004.1980.1
Xcc-8004.1983.1: Xcc-8004.1983.1 - NADH ubiquinone oxidoreductase chain A (EC 1.6.5.3), at 1,908,452 to 1,908,853
Xcc-8004.1983.1
Xcc-8004.1984.1: Xcc-8004.1984.1 - NADH-ubiquinone oxidoreductase chain B (EC 1.6.5.3), at 1,908,844 to 1,909,398
Xcc-8004.1984.1
Position (kb)
1907
1908
1909 Strain fitness (log2 ratio)
-2
-1
0
1 at 1906.772 kb on + strand, within Xcc-8004.1978.1 at 1906.772 kb on + strand, within Xcc-8004.1978.1 at 1906.794 kb on + strand at 1906.794 kb on + strand at 1906.795 kb on - strand at 1906.795 kb on - strand at 1906.795 kb on - strand at 1906.795 kb on - strand at 1906.883 kb on - strand at 1906.941 kb on + strand at 1907.405 kb on - strand, within Xcc-8004.1979.1 at 1907.741 kb on + strand at 1908.304 kb on + strand at 1908.444 kb on + strand at 1908.444 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1664 hydathodes 6 dpi (Dna extract from 8 * 208 hydathodes and pooled together) remove 1,906,772 + Xcc-8004.1978.1 0.88 -1.7 1,906,772 + Xcc-8004.1978.1 0.88 -0.3 1,906,794 + +0.1 1,906,794 + -1.1 1,906,795 - -1.3 1,906,795 - +1.3 1,906,795 - +0.9 1,906,795 - +0.4 1,906,883 - -2.3 1,906,941 + -2.6 1,907,405 - Xcc-8004.1979.1 0.56 +0.3 1,907,741 + -1.1 1,908,304 + +0.9 1,908,444 + -0.5 1,908,444 + -1.7
Or see this region's nucleotide sequence