Experiment: 1664 hydathodes 6 dpi (Dna extract from 8 * 208 hydathodes and pooled together)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Xcc-8004.1020.1 and Xcc-8004.1021.1 are separated by 25 nucleotides Xcc-8004.1021.1 and Xcc-8004.1022.1 overlap by 133 nucleotides Xcc-8004.1022.1 and Xcc-8004.1023.1 are separated by 224 nucleotides
Xcc-8004.1020.1: Xcc-8004.1020.1 - Acetoacetyl-CoA reductase (EC 1.1.1.36), at 968,123 to 968,863
Xcc-8004.1020.1
Xcc-8004.1021.1: Xcc-8004.1021.1 - hypothetical protein, at 968,889 to 969,158
Xcc-8004.1021.1
Xcc-8004.1022.1: Xcc-8004.1022.1 - hypothetical protein, at 969,026 to 970,354
Xcc-8004.1022.1
Xcc-8004.1023.1: Xcc-8004.1023.1 - Phosphate-specific outer membrane porin OprP ; Pyrophosphate-specific outer membrane porin OprO, at 970,579 to 971,772
Xcc-8004.1023.1
Position (kb) 969
970
971 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 968.035 kb on + strand at 968.035 kb on + strand at 968.036 kb on - strand at 968.125 kb on - strand at 968.133 kb on + strand at 968.133 kb on + strand at 968.133 kb on + strand at 968.134 kb on - strand at 968.154 kb on + strand at 968.155 kb on - strand at 968.173 kb on - strand at 968.173 kb on - strand at 968.309 kb on - strand, within Xcc-8004.1020.1 at 968.311 kb on - strand, within Xcc-8004.1020.1 at 968.844 kb on + strand at 968.845 kb on - strand at 968.845 kb on - strand at 969.156 kb on + strand at 969.157 kb on - strand at 969.182 kb on + strand, within Xcc-8004.1022.1 at 969.182 kb on + strand, within Xcc-8004.1022.1 at 969.183 kb on - strand, within Xcc-8004.1022.1 at 969.183 kb on - strand, within Xcc-8004.1022.1 at 969.183 kb on - strand, within Xcc-8004.1022.1 at 969.310 kb on + strand, within Xcc-8004.1022.1 at 969.461 kb on + strand, within Xcc-8004.1022.1 at 969.522 kb on + strand, within Xcc-8004.1022.1 at 969.522 kb on + strand, within Xcc-8004.1022.1 at 969.522 kb on + strand, within Xcc-8004.1022.1 at 969.970 kb on - strand, within Xcc-8004.1022.1 at 970.002 kb on + strand, within Xcc-8004.1022.1 at 970.002 kb on + strand, within Xcc-8004.1022.1 at 970.003 kb on - strand, within Xcc-8004.1022.1 at 970.003 kb on - strand, within Xcc-8004.1022.1 at 970.003 kb on - strand, within Xcc-8004.1022.1 at 970.003 kb on - strand, within Xcc-8004.1022.1 at 970.003 kb on - strand, within Xcc-8004.1022.1 at 970.150 kb on - strand, within Xcc-8004.1022.1 at 970.245 kb on - strand at 970.311 kb on + strand at 970.311 kb on + strand at 970.312 kb on - strand at 970.461 kb on + strand at 970.580 kb on + strand at 970.580 kb on + strand at 970.581 kb on - strand at 970.617 kb on + strand at 970.617 kb on + strand at 970.618 kb on - strand at 970.618 kb on - strand at 970.701 kb on - strand, within Xcc-8004.1023.1 at 970.784 kb on + strand, within Xcc-8004.1023.1 at 970.785 kb on - strand, within Xcc-8004.1023.1 at 970.785 kb on - strand, within Xcc-8004.1023.1 at 970.785 kb on - strand, within Xcc-8004.1023.1 at 970.790 kb on + strand, within Xcc-8004.1023.1 at 970.910 kb on + strand, within Xcc-8004.1023.1 at 970.993 kb on + strand, within Xcc-8004.1023.1 at 970.993 kb on + strand, within Xcc-8004.1023.1 at 970.994 kb on - strand, within Xcc-8004.1023.1 at 971.145 kb on - strand, within Xcc-8004.1023.1 at 971.271 kb on - strand, within Xcc-8004.1023.1 at 971.330 kb on + strand, within Xcc-8004.1023.1
Per-strain Table
Position Strand Gene LocusTag Fraction 1664 hydathodes 6 dpi (Dna extract from 8 * 208 hydathodes and pooled together) remove 968,035 + +0.5 968,035 + -0.2 968,036 - -1.0 968,125 - -0.1 968,133 + -0.5 968,133 + -0.2 968,133 + +2.2 968,134 - -0.0 968,154 + -0.5 968,155 - -1.0 968,173 - -2.6 968,173 - -1.6 968,309 - Xcc-8004.1020.1 0.25 -2.7 968,311 - Xcc-8004.1020.1 0.25 -2.7 968,844 + -2.0 968,845 - -4.1 968,845 - -1.9 969,156 + -0.6 969,157 - -1.1 969,182 + Xcc-8004.1022.1 0.12 -1.9 969,182 + Xcc-8004.1022.1 0.12 -1.9 969,183 - Xcc-8004.1022.1 0.12 -1.1 969,183 - Xcc-8004.1022.1 0.12 -0.6 969,183 - Xcc-8004.1022.1 0.12 -0.1 969,310 + Xcc-8004.1022.1 0.21 -1.9 969,461 + Xcc-8004.1022.1 0.33 -2.2 969,522 + Xcc-8004.1022.1 0.37 -0.9 969,522 + Xcc-8004.1022.1 0.37 -1.6 969,522 + Xcc-8004.1022.1 0.37 -1.1 969,970 - Xcc-8004.1022.1 0.71 -1.6 970,002 + Xcc-8004.1022.1 0.73 -1.1 970,002 + Xcc-8004.1022.1 0.73 -0.1 970,003 - Xcc-8004.1022.1 0.74 -1.6 970,003 - Xcc-8004.1022.1 0.74 -2.6 970,003 - Xcc-8004.1022.1 0.74 -0.4 970,003 - Xcc-8004.1022.1 0.74 -3.6 970,003 - Xcc-8004.1022.1 0.74 -0.8 970,150 - Xcc-8004.1022.1 0.85 -0.9 970,245 - -0.6 970,311 + +0.4 970,311 + -2.2 970,312 - -1.9 970,461 + -2.8 970,580 + -2.1 970,580 + -0.2 970,581 - -1.1 970,617 + +1.2 970,617 + -0.8 970,618 - -0.6 970,618 - -0.9 970,701 - Xcc-8004.1023.1 0.10 +0.4 970,784 + Xcc-8004.1023.1 0.17 +0.2 970,785 - Xcc-8004.1023.1 0.17 +0.2 970,785 - Xcc-8004.1023.1 0.17 -0.3 970,785 - Xcc-8004.1023.1 0.17 -2.8 970,790 + Xcc-8004.1023.1 0.18 +0.5 970,910 + Xcc-8004.1023.1 0.28 -1.3 970,993 + Xcc-8004.1023.1 0.35 -1.3 970,993 + Xcc-8004.1023.1 0.35 -1.4 970,994 - Xcc-8004.1023.1 0.35 +2.4 971,145 - Xcc-8004.1023.1 0.47 +0.4 971,271 - Xcc-8004.1023.1 0.58 -0.5 971,330 + Xcc-8004.1023.1 0.63 -2.5
Or see this region's nucleotide sequence