Experiment: R2A with benzoic 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS22715 and CA265_RS22720 are separated by 75 nucleotides CA265_RS22720 and CA265_RS22725 are separated by 18 nucleotides CA265_RS22725 and CA265_RS22730 are separated by 121 nucleotides
CA265_RS22715: CA265_RS22715 - acyl-CoA dehydrogenase, at 5,560,184 to 5,561,323
_RS22715
CA265_RS22720: CA265_RS22720 - hypothetical protein, at 5,561,399 to 5,561,743
_RS22720
CA265_RS22725: CA265_RS22725 - hypothetical protein, at 5,561,762 to 5,562,193
_RS22725
CA265_RS22730: CA265_RS22730 - ribosome assembly cofactor RimP, at 5,562,315 to 5,562,779
_RS22730
Position (kb) 5561
5562 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5560.454 kb on - strand, within CA265_RS22715 at 5560.515 kb on + strand, within CA265_RS22715 at 5560.586 kb on - strand, within CA265_RS22715 at 5560.622 kb on + strand, within CA265_RS22715 at 5560.625 kb on + strand, within CA265_RS22715 at 5560.626 kb on - strand, within CA265_RS22715 at 5560.632 kb on + strand, within CA265_RS22715 at 5560.718 kb on - strand, within CA265_RS22715 at 5560.724 kb on - strand, within CA265_RS22715 at 5560.724 kb on - strand, within CA265_RS22715 at 5560.724 kb on - strand, within CA265_RS22715 at 5560.726 kb on + strand, within CA265_RS22715 at 5560.735 kb on + strand, within CA265_RS22715 at 5560.818 kb on + strand, within CA265_RS22715 at 5560.826 kb on - strand, within CA265_RS22715 at 5560.940 kb on - strand, within CA265_RS22715 at 5560.973 kb on + strand, within CA265_RS22715 at 5560.974 kb on - strand, within CA265_RS22715 at 5560.979 kb on - strand, within CA265_RS22715 at 5561.119 kb on + strand, within CA265_RS22715 at 5561.120 kb on - strand, within CA265_RS22715 at 5561.120 kb on - strand, within CA265_RS22715 at 5561.147 kb on + strand, within CA265_RS22715 at 5561.193 kb on + strand, within CA265_RS22715 at 5561.223 kb on - strand at 5561.238 kb on + strand at 5561.276 kb on - strand at 5561.317 kb on - strand at 5561.322 kb on - strand at 5561.322 kb on - strand at 5561.373 kb on + strand at 5561.400 kb on + strand at 5561.448 kb on - strand, within CA265_RS22720 at 5561.448 kb on - strand, within CA265_RS22720 at 5561.520 kb on - strand, within CA265_RS22720 at 5561.542 kb on + strand, within CA265_RS22720 at 5561.542 kb on + strand, within CA265_RS22720 at 5561.588 kb on + strand, within CA265_RS22720 at 5561.734 kb on + strand at 5561.734 kb on + strand at 5561.760 kb on - strand at 5561.809 kb on - strand, within CA265_RS22725 at 5561.831 kb on - strand, within CA265_RS22725 at 5561.945 kb on - strand, within CA265_RS22725 at 5561.995 kb on - strand, within CA265_RS22725 at 5561.995 kb on - strand, within CA265_RS22725 at 5562.012 kb on + strand, within CA265_RS22725 at 5562.019 kb on + strand, within CA265_RS22725 at 5562.025 kb on + strand, within CA265_RS22725 at 5562.031 kb on - strand, within CA265_RS22725 at 5562.048 kb on + strand, within CA265_RS22725 at 5562.049 kb on - strand, within CA265_RS22725 at 5562.102 kb on - strand, within CA265_RS22725 at 5562.185 kb on - strand at 5562.206 kb on + strand at 5562.207 kb on - strand at 5562.391 kb on + strand, within CA265_RS22730 at 5562.391 kb on + strand, within CA265_RS22730 at 5562.415 kb on + strand, within CA265_RS22730 at 5562.743 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with benzoic 0.1 mg/ml remove 5,560,454 - CA265_RS22715 0.24 -0.2 5,560,515 + CA265_RS22715 0.29 +0.3 5,560,586 - CA265_RS22715 0.35 +1.0 5,560,622 + CA265_RS22715 0.38 -0.5 5,560,625 + CA265_RS22715 0.39 +0.9 5,560,626 - CA265_RS22715 0.39 -0.3 5,560,632 + CA265_RS22715 0.39 -0.8 5,560,718 - CA265_RS22715 0.47 -0.6 5,560,724 - CA265_RS22715 0.47 -0.9 5,560,724 - CA265_RS22715 0.47 -0.2 5,560,724 - CA265_RS22715 0.47 +0.2 5,560,726 + CA265_RS22715 0.48 +1.9 5,560,735 + CA265_RS22715 0.48 -0.2 5,560,818 + CA265_RS22715 0.56 +0.3 5,560,826 - CA265_RS22715 0.56 +0.2 5,560,940 - CA265_RS22715 0.66 -0.4 5,560,973 + CA265_RS22715 0.69 -0.9 5,560,974 - CA265_RS22715 0.69 +0.3 5,560,979 - CA265_RS22715 0.70 +1.1 5,561,119 + CA265_RS22715 0.82 +1.1 5,561,120 - CA265_RS22715 0.82 +0.0 5,561,120 - CA265_RS22715 0.82 +1.0 5,561,147 + CA265_RS22715 0.84 +0.2 5,561,193 + CA265_RS22715 0.89 -0.6 5,561,223 - +0.1 5,561,238 + -1.7 5,561,276 - -0.8 5,561,317 - +1.3 5,561,322 - +0.4 5,561,322 - +0.1 5,561,373 + -0.0 5,561,400 + +2.1 5,561,448 - CA265_RS22720 0.14 -0.1 5,561,448 - CA265_RS22720 0.14 +0.4 5,561,520 - CA265_RS22720 0.35 -0.1 5,561,542 + CA265_RS22720 0.41 +0.8 5,561,542 + CA265_RS22720 0.41 +0.2 5,561,588 + CA265_RS22720 0.55 +0.0 5,561,734 + +0.7 5,561,734 + -1.3 5,561,760 - +0.2 5,561,809 - CA265_RS22725 0.11 -0.9 5,561,831 - CA265_RS22725 0.16 +0.9 5,561,945 - CA265_RS22725 0.42 -0.2 5,561,995 - CA265_RS22725 0.54 +0.2 5,561,995 - CA265_RS22725 0.54 -0.5 5,562,012 + CA265_RS22725 0.58 -0.9 5,562,019 + CA265_RS22725 0.59 -0.8 5,562,025 + CA265_RS22725 0.61 -0.0 5,562,031 - CA265_RS22725 0.62 -0.2 5,562,048 + CA265_RS22725 0.66 -1.7 5,562,049 - CA265_RS22725 0.66 +0.2 5,562,102 - CA265_RS22725 0.79 +0.3 5,562,185 - -0.3 5,562,206 + -0.2 5,562,207 - -0.2 5,562,391 + CA265_RS22730 0.16 -0.2 5,562,391 + CA265_RS22730 0.16 -0.9 5,562,415 + CA265_RS22730 0.22 -0.7 5,562,743 + -1.8
Or see this region's nucleotide sequence