Experiment: R2A with Aluminum chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS19645 and CA265_RS19650 overlap by 1 nucleotides CA265_RS19650 and CA265_RS19655 are separated by 174 nucleotides CA265_RS19655 and CA265_RS19660 are separated by 51 nucleotides CA265_RS19660 and CA265_RS19665 overlap by 11 nucleotides CA265_RS19665 and CA265_RS19670 overlap by 1 nucleotides
CA265_RS19645: CA265_RS19645 - GNAT family N-acetyltransferase, at 4,867,494 to 4,868,033
_RS19645
CA265_RS19650: CA265_RS19650 - DNA-3-methyladenine glycosylase, at 4,868,033 to 4,868,596
_RS19650
CA265_RS19655: CA265_RS19655 - carboxymuconolactone decarboxylase family protein, at 4,868,771 to 4,869,124
_RS19655
CA265_RS19660: CA265_RS19660 - hypothetical protein, at 4,869,176 to 4,869,535
_RS19660
CA265_RS19665: CA265_RS19665 - cysteine methyltransferase, at 4,869,525 to 4,869,857
_RS19665
CA265_RS19670: CA265_RS19670 - tRNA (guanosine(46)-N7)-methyltransferase TrmB, at 4,869,857 to 4,870,507
_RS19670
Position (kb)
4868
4869
4870 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4867.801 kb on - strand, within CA265_RS19645 at 4867.894 kb on + strand, within CA265_RS19645 at 4867.922 kb on - strand, within CA265_RS19645 at 4868.018 kb on + strand at 4868.019 kb on - strand at 4868.035 kb on - strand at 4868.143 kb on + strand, within CA265_RS19650 at 4868.159 kb on + strand, within CA265_RS19650 at 4868.160 kb on - strand, within CA265_RS19650 at 4868.241 kb on + strand, within CA265_RS19650 at 4868.242 kb on - strand, within CA265_RS19650 at 4868.357 kb on + strand, within CA265_RS19650 at 4868.357 kb on + strand, within CA265_RS19650 at 4868.381 kb on + strand, within CA265_RS19650 at 4868.509 kb on - strand, within CA265_RS19650 at 4868.538 kb on + strand, within CA265_RS19650 at 4868.538 kb on + strand, within CA265_RS19650 at 4868.539 kb on - strand, within CA265_RS19650 at 4868.582 kb on + strand at 4868.597 kb on - strand at 4868.600 kb on - strand at 4868.628 kb on + strand at 4868.763 kb on - strand at 4868.773 kb on - strand at 4868.773 kb on - strand at 4868.835 kb on - strand, within CA265_RS19655 at 4868.835 kb on - strand, within CA265_RS19655 at 4868.842 kb on + strand, within CA265_RS19655 at 4868.894 kb on + strand, within CA265_RS19655 at 4868.915 kb on - strand, within CA265_RS19655 at 4868.929 kb on + strand, within CA265_RS19655 at 4869.047 kb on - strand, within CA265_RS19655 at 4869.141 kb on + strand at 4869.219 kb on + strand, within CA265_RS19660 at 4869.287 kb on + strand, within CA265_RS19660 at 4869.329 kb on - strand, within CA265_RS19660 at 4869.397 kb on + strand, within CA265_RS19660 at 4869.397 kb on + strand, within CA265_RS19660 at 4869.397 kb on + strand, within CA265_RS19660 at 4869.421 kb on + strand, within CA265_RS19660 at 4869.475 kb on - strand, within CA265_RS19660 at 4869.506 kb on + strand at 4869.527 kb on - strand at 4869.572 kb on + strand, within CA265_RS19665 at 4869.678 kb on + strand, within CA265_RS19665 at 4869.679 kb on - strand, within CA265_RS19665 at 4869.682 kb on - strand, within CA265_RS19665 at 4869.682 kb on - strand, within CA265_RS19665 at 4869.722 kb on - strand, within CA265_RS19665 at 4869.830 kb on - strand at 4869.878 kb on - strand at 4869.879 kb on + strand at 4869.899 kb on + strand at 4869.938 kb on + strand, within CA265_RS19670 at 4869.963 kb on + strand, within CA265_RS19670 at 4869.986 kb on + strand, within CA265_RS19670 at 4869.987 kb on - strand, within CA265_RS19670 at 4870.021 kb on + strand, within CA265_RS19670 at 4870.024 kb on - strand, within CA265_RS19670 at 4870.024 kb on - strand, within CA265_RS19670
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Aluminum chloride 1 mM remove 4,867,801 - CA265_RS19645 0.57 -0.8 4,867,894 + CA265_RS19645 0.74 +0.2 4,867,922 - CA265_RS19645 0.79 +0.1 4,868,018 + +0.3 4,868,019 - +0.9 4,868,035 - -0.6 4,868,143 + CA265_RS19650 0.20 -0.0 4,868,159 + CA265_RS19650 0.22 +0.9 4,868,160 - CA265_RS19650 0.23 -1.4 4,868,241 + CA265_RS19650 0.37 -0.7 4,868,242 - CA265_RS19650 0.37 -0.0 4,868,357 + CA265_RS19650 0.57 +0.3 4,868,357 + CA265_RS19650 0.57 +0.8 4,868,381 + CA265_RS19650 0.62 +0.0 4,868,509 - CA265_RS19650 0.84 -1.7 4,868,538 + CA265_RS19650 0.90 -0.2 4,868,538 + CA265_RS19650 0.90 +0.6 4,868,539 - CA265_RS19650 0.90 +0.5 4,868,582 + +0.9 4,868,597 - +1.9 4,868,600 - +0.1 4,868,628 + +0.1 4,868,763 - -0.1 4,868,773 - +0.1 4,868,773 - +0.6 4,868,835 - CA265_RS19655 0.18 -0.1 4,868,835 - CA265_RS19655 0.18 +0.6 4,868,842 + CA265_RS19655 0.20 +0.8 4,868,894 + CA265_RS19655 0.35 +0.5 4,868,915 - CA265_RS19655 0.41 -0.0 4,868,929 + CA265_RS19655 0.45 -0.2 4,869,047 - CA265_RS19655 0.78 +1.2 4,869,141 + +0.8 4,869,219 + CA265_RS19660 0.12 +0.7 4,869,287 + CA265_RS19660 0.31 +0.0 4,869,329 - CA265_RS19660 0.42 +0.0 4,869,397 + CA265_RS19660 0.61 +0.6 4,869,397 + CA265_RS19660 0.61 -0.0 4,869,397 + CA265_RS19660 0.61 +0.2 4,869,421 + CA265_RS19660 0.68 +0.0 4,869,475 - CA265_RS19660 0.83 -0.1 4,869,506 + +0.2 4,869,527 - +0.9 4,869,572 + CA265_RS19665 0.14 +0.2 4,869,678 + CA265_RS19665 0.46 -2.0 4,869,679 - CA265_RS19665 0.46 +0.7 4,869,682 - CA265_RS19665 0.47 -0.2 4,869,682 - CA265_RS19665 0.47 +1.4 4,869,722 - CA265_RS19665 0.59 +1.3 4,869,830 - -2.2 4,869,878 - -1.1 4,869,879 + -2.8 4,869,899 + +0.5 4,869,938 + CA265_RS19670 0.12 +0.7 4,869,963 + CA265_RS19670 0.16 -0.8 4,869,986 + CA265_RS19670 0.20 -0.8 4,869,987 - CA265_RS19670 0.20 +0.2 4,870,021 + CA265_RS19670 0.25 +0.1 4,870,024 - CA265_RS19670 0.26 +0.1 4,870,024 - CA265_RS19670 0.26 -0.1
Or see this region's nucleotide sequence