Experiment: R2A with Aluminum chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS05715 and CA265_RS05720 are separated by 681 nucleotides CA265_RS05720 and CA265_RS05725 are separated by 9 nucleotides CA265_RS05725 and CA265_RS05730 are separated by 106 nucleotides
CA265_RS05715: CA265_RS05715 - amino acid dehydrogenase, at 1,389,385 to 1,390,626
_RS05715
CA265_RS05720: CA265_RS05720 - hypothetical protein, at 1,391,308 to 1,393,236
_RS05720
CA265_RS05725: CA265_RS05725 - hypothetical protein, at 1,393,246 to 1,393,818
_RS05725
CA265_RS05730: CA265_RS05730 - hypothetical protein, at 1,393,925 to 1,394,275
_RS05730
Position (kb)
1391
1392
1393
1394 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 1390.330 kb on - strand, within CA265_RS05715 at 1390.330 kb on - strand, within CA265_RS05715 at 1390.362 kb on + strand, within CA265_RS05715 at 1390.433 kb on - strand, within CA265_RS05715 at 1390.468 kb on + strand, within CA265_RS05715 at 1390.469 kb on - strand, within CA265_RS05715 at 1390.624 kb on + strand at 1390.630 kb on - strand at 1390.630 kb on - strand at 1390.632 kb on + strand at 1390.661 kb on - strand at 1390.734 kb on + strand at 1390.758 kb on - strand at 1390.793 kb on + strand at 1390.798 kb on + strand at 1390.810 kb on + strand at 1390.810 kb on + strand at 1390.876 kb on + strand at 1390.877 kb on - strand at 1390.882 kb on - strand at 1390.920 kb on + strand at 1390.928 kb on + strand at 1391.117 kb on + strand at 1391.301 kb on + strand at 1391.302 kb on - strand at 1391.341 kb on + strand at 1391.386 kb on - strand at 1391.428 kb on - strand at 1391.517 kb on + strand, within CA265_RS05720 at 1391.552 kb on + strand, within CA265_RS05720 at 1391.552 kb on + strand, within CA265_RS05720 at 1391.595 kb on + strand, within CA265_RS05720 at 1391.617 kb on - strand, within CA265_RS05720 at 1391.617 kb on - strand, within CA265_RS05720 at 1391.660 kb on + strand, within CA265_RS05720 at 1391.670 kb on + strand, within CA265_RS05720 at 1391.670 kb on + strand, within CA265_RS05720 at 1391.675 kb on + strand, within CA265_RS05720 at 1391.676 kb on - strand, within CA265_RS05720 at 1391.766 kb on + strand, within CA265_RS05720 at 1391.767 kb on - strand, within CA265_RS05720 at 1391.803 kb on + strand, within CA265_RS05720 at 1391.806 kb on - strand, within CA265_RS05720 at 1391.848 kb on + strand, within CA265_RS05720 at 1391.849 kb on - strand, within CA265_RS05720 at 1391.849 kb on - strand, within CA265_RS05720 at 1391.850 kb on + strand, within CA265_RS05720 at 1391.862 kb on + strand, within CA265_RS05720 at 1391.863 kb on - strand, within CA265_RS05720 at 1391.867 kb on + strand, within CA265_RS05720 at 1391.867 kb on + strand, within CA265_RS05720 at 1391.904 kb on - strand, within CA265_RS05720 at 1391.965 kb on + strand, within CA265_RS05720 at 1391.965 kb on + strand, within CA265_RS05720 at 1391.965 kb on + strand, within CA265_RS05720 at 1391.988 kb on + strand, within CA265_RS05720 at 1391.988 kb on + strand, within CA265_RS05720 at 1392.045 kb on - strand, within CA265_RS05720 at 1392.093 kb on + strand, within CA265_RS05720 at 1392.126 kb on + strand, within CA265_RS05720 at 1392.128 kb on + strand, within CA265_RS05720 at 1392.128 kb on + strand, within CA265_RS05720 at 1392.178 kb on + strand, within CA265_RS05720 at 1392.179 kb on - strand, within CA265_RS05720 at 1392.180 kb on + strand, within CA265_RS05720 at 1392.181 kb on - strand, within CA265_RS05720 at 1392.182 kb on + strand, within CA265_RS05720 at 1392.229 kb on + strand, within CA265_RS05720 at 1392.231 kb on + strand, within CA265_RS05720 at 1392.232 kb on - strand, within CA265_RS05720 at 1392.234 kb on + strand, within CA265_RS05720 at 1392.281 kb on + strand, within CA265_RS05720 at 1392.314 kb on - strand, within CA265_RS05720 at 1392.314 kb on - strand, within CA265_RS05720 at 1392.315 kb on + strand, within CA265_RS05720 at 1392.328 kb on - strand, within CA265_RS05720 at 1392.328 kb on - strand, within CA265_RS05720 at 1392.330 kb on + strand, within CA265_RS05720 at 1392.331 kb on - strand, within CA265_RS05720 at 1392.339 kb on + strand, within CA265_RS05720 at 1392.350 kb on + strand, within CA265_RS05720 at 1392.355 kb on + strand, within CA265_RS05720 at 1392.398 kb on + strand, within CA265_RS05720 at 1392.422 kb on + strand, within CA265_RS05720 at 1392.423 kb on - strand, within CA265_RS05720 at 1392.469 kb on + strand, within CA265_RS05720 at 1392.470 kb on - strand, within CA265_RS05720 at 1392.472 kb on - strand, within CA265_RS05720 at 1392.479 kb on - strand, within CA265_RS05720 at 1392.609 kb on + strand, within CA265_RS05720 at 1392.614 kb on - strand, within CA265_RS05720 at 1392.678 kb on + strand, within CA265_RS05720 at 1392.679 kb on - strand, within CA265_RS05720 at 1392.679 kb on - strand, within CA265_RS05720 at 1392.684 kb on - strand, within CA265_RS05720 at 1392.706 kb on - strand, within CA265_RS05720 at 1392.714 kb on - strand, within CA265_RS05720 at 1392.751 kb on - strand, within CA265_RS05720 at 1392.755 kb on + strand, within CA265_RS05720 at 1392.755 kb on + strand, within CA265_RS05720 at 1392.837 kb on + strand, within CA265_RS05720 at 1392.838 kb on - strand, within CA265_RS05720 at 1392.922 kb on - strand, within CA265_RS05720 at 1392.930 kb on + strand, within CA265_RS05720 at 1392.972 kb on + strand, within CA265_RS05720 at 1392.978 kb on - strand, within CA265_RS05720 at 1392.978 kb on - strand, within CA265_RS05720 at 1393.012 kb on + strand, within CA265_RS05720 at 1393.013 kb on - strand, within CA265_RS05720 at 1393.013 kb on - strand, within CA265_RS05720 at 1393.013 kb on - strand, within CA265_RS05720 at 1393.013 kb on - strand, within CA265_RS05720 at 1393.014 kb on + strand, within CA265_RS05720 at 1393.015 kb on - strand, within CA265_RS05720 at 1393.018 kb on - strand, within CA265_RS05720 at 1393.032 kb on + strand at 1393.061 kb on + strand at 1393.062 kb on - strand at 1393.154 kb on + strand at 1393.191 kb on + strand at 1393.207 kb on - strand at 1393.209 kb on - strand at 1393.240 kb on + strand at 1393.240 kb on + strand at 1393.241 kb on - strand at 1393.241 kb on - strand at 1393.317 kb on + strand, within CA265_RS05725 at 1393.374 kb on + strand, within CA265_RS05725 at 1393.378 kb on + strand, within CA265_RS05725 at 1393.379 kb on - strand, within CA265_RS05725 at 1393.406 kb on - strand, within CA265_RS05725 at 1393.406 kb on - strand, within CA265_RS05725 at 1393.473 kb on + strand, within CA265_RS05725 at 1393.529 kb on - strand, within CA265_RS05725 at 1393.540 kb on - strand, within CA265_RS05725 at 1393.543 kb on - strand, within CA265_RS05725 at 1393.557 kb on - strand, within CA265_RS05725 at 1393.594 kb on - strand, within CA265_RS05725 at 1393.594 kb on - strand, within CA265_RS05725 at 1393.596 kb on + strand, within CA265_RS05725 at 1393.597 kb on - strand, within CA265_RS05725 at 1393.705 kb on - strand, within CA265_RS05725 at 1393.823 kb on + strand at 1393.827 kb on + strand at 1393.885 kb on + strand at 1393.885 kb on + strand at 1393.886 kb on - strand at 1393.890 kb on - strand at 1393.905 kb on - strand at 1393.909 kb on - strand at 1393.910 kb on + strand at 1393.922 kb on + strand at 1393.923 kb on - strand at 1393.969 kb on + strand, within CA265_RS05730 at 1393.970 kb on - strand, within CA265_RS05730 at 1393.970 kb on - strand, within CA265_RS05730 at 1393.988 kb on - strand, within CA265_RS05730 at 1394.031 kb on - strand, within CA265_RS05730 at 1394.033 kb on - strand, within CA265_RS05730 at 1394.085 kb on + strand, within CA265_RS05730 at 1394.111 kb on - strand, within CA265_RS05730 at 1394.134 kb on + strand, within CA265_RS05730 at 1394.136 kb on + strand, within CA265_RS05730 at 1394.137 kb on - strand, within CA265_RS05730 at 1394.159 kb on - strand, within CA265_RS05730 at 1394.159 kb on - strand, within CA265_RS05730
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Aluminum chloride 1 mM remove 1,390,330 - CA265_RS05715 0.76 +0.3 1,390,330 - CA265_RS05715 0.76 -0.1 1,390,362 + CA265_RS05715 0.79 +0.7 1,390,433 - CA265_RS05715 0.84 -0.2 1,390,468 + CA265_RS05715 0.87 +1.1 1,390,469 - CA265_RS05715 0.87 -0.2 1,390,624 + +0.6 1,390,630 - +0.3 1,390,630 - -1.5 1,390,632 + +0.8 1,390,661 - +0.0 1,390,734 + +1.1 1,390,758 - +0.9 1,390,793 + +1.1 1,390,798 + +1.0 1,390,810 + +1.1 1,390,810 + +1.0 1,390,876 + +0.4 1,390,877 - -0.3 1,390,882 - -0.5 1,390,920 + +0.9 1,390,928 + +0.8 1,391,117 + +1.0 1,391,301 + +0.7 1,391,302 - -1.7 1,391,341 + -1.9 1,391,386 - -1.2 1,391,428 - -0.9 1,391,517 + CA265_RS05720 0.11 +0.1 1,391,552 + CA265_RS05720 0.13 +0.3 1,391,552 + CA265_RS05720 0.13 +0.8 1,391,595 + CA265_RS05720 0.15 +0.8 1,391,617 - CA265_RS05720 0.16 -3.8 1,391,617 - CA265_RS05720 0.16 -3.2 1,391,660 + CA265_RS05720 0.18 +0.4 1,391,670 + CA265_RS05720 0.19 +1.2 1,391,670 + CA265_RS05720 0.19 +0.4 1,391,675 + CA265_RS05720 0.19 +0.7 1,391,676 - CA265_RS05720 0.19 -2.9 1,391,766 + CA265_RS05720 0.24 -0.8 1,391,767 - CA265_RS05720 0.24 -3.7 1,391,803 + CA265_RS05720 0.26 -0.3 1,391,806 - CA265_RS05720 0.26 -0.2 1,391,848 + CA265_RS05720 0.28 +1.2 1,391,849 - CA265_RS05720 0.28 -4.1 1,391,849 - CA265_RS05720 0.28 -3.2 1,391,850 + CA265_RS05720 0.28 +2.4 1,391,862 + CA265_RS05720 0.29 +0.6 1,391,863 - CA265_RS05720 0.29 -2.8 1,391,867 + CA265_RS05720 0.29 +0.9 1,391,867 + CA265_RS05720 0.29 +0.5 1,391,904 - CA265_RS05720 0.31 -4.4 1,391,965 + CA265_RS05720 0.34 +0.6 1,391,965 + CA265_RS05720 0.34 +0.5 1,391,965 + CA265_RS05720 0.34 +0.7 1,391,988 + CA265_RS05720 0.35 +1.9 1,391,988 + CA265_RS05720 0.35 +1.2 1,392,045 - CA265_RS05720 0.38 +0.6 1,392,093 + CA265_RS05720 0.41 +1.9 1,392,126 + CA265_RS05720 0.42 +0.9 1,392,128 + CA265_RS05720 0.43 +1.5 1,392,128 + CA265_RS05720 0.43 +1.4 1,392,178 + CA265_RS05720 0.45 +0.7 1,392,179 - CA265_RS05720 0.45 -2.4 1,392,180 + CA265_RS05720 0.45 +0.9 1,392,181 - CA265_RS05720 0.45 -1.9 1,392,182 + CA265_RS05720 0.45 -0.9 1,392,229 + CA265_RS05720 0.48 +1.0 1,392,231 + CA265_RS05720 0.48 +0.1 1,392,232 - CA265_RS05720 0.48 -3.1 1,392,234 + CA265_RS05720 0.48 +0.8 1,392,281 + CA265_RS05720 0.50 +0.8 1,392,314 - CA265_RS05720 0.52 -4.4 1,392,314 - CA265_RS05720 0.52 -0.9 1,392,315 + CA265_RS05720 0.52 +1.2 1,392,328 - CA265_RS05720 0.53 -2.7 1,392,328 - CA265_RS05720 0.53 -3.2 1,392,330 + CA265_RS05720 0.53 +0.1 1,392,331 - CA265_RS05720 0.53 -4.1 1,392,339 + CA265_RS05720 0.53 +0.9 1,392,350 + CA265_RS05720 0.54 +0.8 1,392,355 + CA265_RS05720 0.54 +2.1 1,392,398 + CA265_RS05720 0.57 +0.5 1,392,422 + CA265_RS05720 0.58 +0.5 1,392,423 - CA265_RS05720 0.58 -3.5 1,392,469 + CA265_RS05720 0.60 +1.5 1,392,470 - CA265_RS05720 0.60 -4.2 1,392,472 - CA265_RS05720 0.60 -2.4 1,392,479 - CA265_RS05720 0.61 -0.2 1,392,609 + CA265_RS05720 0.67 +1.1 1,392,614 - CA265_RS05720 0.68 -3.2 1,392,678 + CA265_RS05720 0.71 +0.9 1,392,679 - CA265_RS05720 0.71 -3.2 1,392,679 - CA265_RS05720 0.71 -3.2 1,392,684 - CA265_RS05720 0.71 -5.1 1,392,706 - CA265_RS05720 0.72 -5.7 1,392,714 - CA265_RS05720 0.73 -0.2 1,392,751 - CA265_RS05720 0.75 -2.6 1,392,755 + CA265_RS05720 0.75 +0.6 1,392,755 + CA265_RS05720 0.75 +1.0 1,392,837 + CA265_RS05720 0.79 +0.7 1,392,838 - CA265_RS05720 0.79 -3.3 1,392,922 - CA265_RS05720 0.84 -3.0 1,392,930 + CA265_RS05720 0.84 +0.6 1,392,972 + CA265_RS05720 0.86 +0.6 1,392,978 - CA265_RS05720 0.87 -3.6 1,392,978 - CA265_RS05720 0.87 -2.3 1,393,012 + CA265_RS05720 0.88 +0.8 1,393,013 - CA265_RS05720 0.88 -2.8 1,393,013 - CA265_RS05720 0.88 -3.4 1,393,013 - CA265_RS05720 0.88 -1.6 1,393,013 - CA265_RS05720 0.88 -1.8 1,393,014 + CA265_RS05720 0.88 +0.9 1,393,015 - CA265_RS05720 0.88 -1.2 1,393,018 - CA265_RS05720 0.89 -2.1 1,393,032 + +0.7 1,393,061 + +0.6 1,393,062 - -2.8 1,393,154 + +1.3 1,393,191 + +1.3 1,393,207 - -3.5 1,393,209 - -3.8 1,393,240 + -2.6 1,393,240 + -4.6 1,393,241 - -4.5 1,393,241 - -3.8 1,393,317 + CA265_RS05725 0.12 -2.5 1,393,374 + CA265_RS05725 0.22 -4.1 1,393,378 + CA265_RS05725 0.23 -2.1 1,393,379 - CA265_RS05725 0.23 -4.3 1,393,406 - CA265_RS05725 0.28 -5.2 1,393,406 - CA265_RS05725 0.28 -3.7 1,393,473 + CA265_RS05725 0.40 -3.1 1,393,529 - CA265_RS05725 0.49 -1.8 1,393,540 - CA265_RS05725 0.51 -2.7 1,393,543 - CA265_RS05725 0.52 -2.3 1,393,557 - CA265_RS05725 0.54 -3.7 1,393,594 - CA265_RS05725 0.61 -4.2 1,393,594 - CA265_RS05725 0.61 -3.0 1,393,596 + CA265_RS05725 0.61 -2.3 1,393,597 - CA265_RS05725 0.61 -5.4 1,393,705 - CA265_RS05725 0.80 -6.3 1,393,823 + -1.0 1,393,827 + -0.7 1,393,885 + -0.3 1,393,885 + +0.2 1,393,886 - -3.9 1,393,890 - -1.4 1,393,905 - -2.9 1,393,909 - -3.6 1,393,910 + +0.2 1,393,922 + +0.4 1,393,923 - -4.8 1,393,969 + CA265_RS05730 0.13 -0.4 1,393,970 - CA265_RS05730 0.13 -3.1 1,393,970 - CA265_RS05730 0.13 -0.5 1,393,988 - CA265_RS05730 0.18 -3.6 1,394,031 - CA265_RS05730 0.30 -3.0 1,394,033 - CA265_RS05730 0.31 -1.4 1,394,085 + CA265_RS05730 0.46 -0.4 1,394,111 - CA265_RS05730 0.53 -2.5 1,394,134 + CA265_RS05730 0.60 -0.1 1,394,136 + CA265_RS05730 0.60 -0.1 1,394,137 - CA265_RS05730 0.60 -3.4 1,394,159 - CA265_RS05730 0.67 -2.1 1,394,159 - CA265_RS05730 0.67 -1.6
Or see this region's nucleotide sequence